Two-dimensional Electrophoresis Analysis of Proteins Extracted from Alexandrium sp. LC3
Two-dimensional electrophoresis（2-DE） of protein extracted and purified from Alexandrium sp. LC3 was conducted. In the SDS-PAGE study, the relative molecular weights of the proteins were mainly in the range of 14 kDa-31 kDa and 43 kDa-66 kDa, and more proteins were detected between 14kDa and 31 kDa....
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| Veröffentlicht in: | Journal of Ocean University of China 2007-10, Vol.6 (4), p.365-368 |
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| creator | Li, Hao Miao, Jinlai Cui, Fengxia Li, Guangyou |
| description | Two-dimensional electrophoresis（2-DE） of protein extracted and purified from Alexandrium sp. LC3 was conducted. In the SDS-PAGE study, the relative molecular weights of the proteins were mainly in the range of 14 kDa-31 kDa and 43 kDa-66 kDa, and more proteins were detected between 14kDa and 31 kDa. With the improved protein preparation, the two-dimensional electrophoresis patterns indicated that the relative molecular weights of the proteins were between 14kDa and 100kDa, and most of them ranged from 14 kDa to 31 kDa. This was consistent with the result of the SDS-PAGE analysis. The isoelectric points were found to lie between 3.0 and 8.0, and most of them were in the range of 3.0-6.0. Better separation effect was acquired with pre-prepared immobilized gradient （IPG） strip （pH 3-5.6）, and about 320 protein spots could be visualized on the 2-DE map by staining. Within pH 3-l0 and pH 3-5.6 strips, the protein samples of Alexandrium sp. LC3 could be separated well. |
| doi_str_mv | 10.1007/s11802-007-0365-2 |
| format | Article |
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LC3</title><source>SpringerLink Journals (MCLS)</source><source>Alma/SFX Local Collection</source><creator>Li, Hao ; Miao, Jinlai ; Cui, Fengxia ; Li, Guangyou</creator><creatorcontrib>Li, Hao ; Miao, Jinlai ; Cui, Fengxia ; Li, Guangyou</creatorcontrib><description>Two-dimensional electrophoresis（2-DE） of protein extracted and purified from Alexandrium sp. LC3 was conducted. In the SDS-PAGE study, the relative molecular weights of the proteins were mainly in the range of 14 kDa-31 kDa and 43 kDa-66 kDa, and more proteins were detected between 14kDa and 31 kDa. With the improved protein preparation, the two-dimensional electrophoresis patterns indicated that the relative molecular weights of the proteins were between 14kDa and 100kDa, and most of them ranged from 14 kDa to 31 kDa. This was consistent with the result of the SDS-PAGE analysis. The isoelectric points were found to lie between 3.0 and 8.0, and most of them were in the range of 3.0-6.0. Better separation effect was acquired with pre-prepared immobilized gradient （IPG） strip （pH 3-5.6）, and about 320 protein spots could be visualized on the 2-DE map by staining. Within pH 3-l0 and pH 3-5.6 strips, the protein samples of Alexandrium sp. LC3 could be separated well.</description><identifier>ISSN: 1672-5182</identifier><identifier>EISSN: 1993-5021</identifier><identifier>EISSN: 1672-5174</identifier><identifier>DOI: 10.1007/s11802-007-0365-2</identifier><language>eng</language><publisher>Dordrecht: Springer Nature B.V</publisher><subject>2维空间电泳 ; Alexandrium ; Electrophoresis ; Marine ; Proteins ; 生物学 ; 蛋白质</subject><ispartof>Journal of Ocean University of China, 2007-10, Vol.6 (4), p.365-368</ispartof><rights>Science Press 2007</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c2338-71b4fd8457f97367bd177d3e44e27aed6d3bb00025f1fd46aa30dc1893032e373</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/87473A/87473A.jpg</thumbnail><link.rule.ids>314,780,784,27915,27916</link.rule.ids></links><search><creatorcontrib>Li, Hao</creatorcontrib><creatorcontrib>Miao, Jinlai</creatorcontrib><creatorcontrib>Cui, Fengxia</creatorcontrib><creatorcontrib>Li, Guangyou</creatorcontrib><title>Two-dimensional Electrophoresis Analysis of Proteins Extracted from Alexandrium sp. LC3</title><title>Journal of Ocean University of China</title><addtitle>Journal of Ocean University of China</addtitle><description>Two-dimensional electrophoresis（2-DE） of protein extracted and purified from Alexandrium sp. LC3 was conducted. 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| subjects | 2维空间电泳 Alexandrium Electrophoresis Marine Proteins 生物学 蛋白质 |
| title | Two-dimensional Electrophoresis Analysis of Proteins Extracted from Alexandrium sp. LC3 |
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