Tunable microfluidic chip for single-cell deformation study
Microfluidic phenotyping methods have been of vital importance for cellular characterization, especially for evaluating single cells. In order to study the deformability of a single cell, we devised and tested a tunable microfluidic chip-based method. A pneumatic polymer polydimethylsiloxane (PDMS)...
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| Veröffentlicht in: | Nanotechnology and Precision Engineering 2023-06, Vol.6 (2), p.25-32 |
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| container_title | Nanotechnology and Precision Engineering |
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| creator | Zhang, Ruiyun Duan, Xuexin Zhang, Shuaihua Guo, Wenlan Sun, Chen Han, Ziyu |
| description | Microfluidic phenotyping methods have been of vital importance for cellular characterization, especially for evaluating single cells. In order to study the deformability of a single cell, we devised and tested a tunable microfluidic chip-based method. A pneumatic polymer polydimethylsiloxane (PDMS) membrane was designed and fabricated abutting a single-cell trapping structure, so the cell could be squeezed controllably in a lateral direction. Cell contour changes under increasing pressure were recorded, enabling the deformation degree of different types of single cell to be analyzed and compared using computer vision. This provides a new perspective for studying mechanical properties of cells at the single cell level. |
| doi_str_mv | 10.1063/10.0017649 |
| format | Article |
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| title | Tunable microfluidic chip for single-cell deformation study |
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