Molecular characterization and functional analysis of daf-8 in the pinewood nematode, Bursaphelenchus xylophilus
Bursaphelenchus xylophilus , causal agent of pine wilt disease, causes extensive damage worldwide. Strategies are needed to inhibit population growth or block the spread of the invasive nematode to control pine wilt disease. The gene daf-8 plays crucial roles in larval development and dauer formatio...
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| Veröffentlicht in: | Journal of forestry research 2022-04, Vol.33 (2), p.689-698 |
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| creator | Wang, Jinghan Hong, Huan Xie, Rong Ji, Jingjing Guo, Kai Bai, Liqun Tang, Jia Yu, Hongshi Ye, Jianren Hu, Jiafu |
| description | Bursaphelenchus xylophilus
, causal agent of pine wilt disease, causes extensive damage worldwide. Strategies are needed to inhibit population growth or block the spread of the invasive nematode to control pine wilt disease. The gene
daf-8
plays crucial roles in larval development and dauer formation in
Caenorhabditis elegans
, but little is known about its orthologue in
B. xylophilus
. In the present molecular characterization and functional analysis of
daf-8
in
B. xylophilus
(
Bx-daf-8
), RT-qPCR showed that the expression of
Bx-daf-8
gradually increased during the embryonic stage, peaked in the second-stage juvenile (J2), then dramatically dropped in the J3, and remained at that low level for the rest of its life.
Bx-daf-8-
transgenic
C. elegans
was employed to mimic the spatiotemporal expression of
Bx-daf-8
, which was expressed close to the pharynx during early embryogenesis and weakly throughout the whole body during late embryogenesis. It was observed in head neurons and tail ganglions throughout all postembryonic stages.
B. xylophilus
embryos were severely abnormal, and hatching rate decreased sharply after
Bx-daf-8
knockdown.
daf-16-1
and
da-f16-2
, downstream genes in the IIS pathway, also dropped sharply after
Bx-daf-8
knockdown. We propose that TGFβ may crosstalk with the IIS pathway upstream of
Bx-daf-16
and that
daf-8
may act as a master regulator of
daf-16
in
B. xylophilus
. However, knockdown of
Bx-daf-8
did not lead to constitutive developmental arrest at the dauer larval stage, indicating that dauer entry in
B. xylophilus
might be controlled by several genes and is more complicated than in
C. elegans
.
Bx-daf-8
alone did not control the dauer entry in
B. xylophilus
, but it was indispensable for embryogenesis, providing a potential target for suppressing population growth of
B. xylophilus
. |
| doi_str_mv | 10.1007/s11676-021-01335-7 |
| format | Article |
| fullrecord | <record><control><sourceid>wanfang_jour_proqu</sourceid><recordid>TN_cdi_wanfang_journals_lyyj202202027</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A706661965</galeid><wanfj_id>lyyj202202027</wanfj_id><sourcerecordid>lyyj202202027</sourcerecordid><originalsourceid>FETCH-LOGICAL-c416t-19325a972d270d01dfdb65c0242ce8187be81473e3fe765411cb1d795dcadfa43</originalsourceid><addsrcrecordid>eNp9kV1rFDEUhgexYK3-Aa8C4p2p-ZhJdi5rUStUvGmhd-FsPnayZJMxmaGOv95sR1gEkRySc8LzhpPzNs0bSi4pIfJDoVRIgQmjmFDOOyyfNee07zkmgsjnNa8UFoI9vGhelrInpGs5b8-b8VsKVs8BMtIDZNCTzf4XTD5FBNEgN0d9LCDUEsJSfEHJIQMOb5CPaBosGn20jykZFO0BpmTse_RxzgXGwQYb9TAX9HMJaRx8mMur5sxBKPb1n_Oiuf_86e76Bt9-__L1-uoW65aKCdOesw56yQyTxBBqnNmKThPWMm03dCO3dW8lt9xZKbqWUr2lRvad0WActPyiebe--wjRQdypfZpz_UFRYVn2jLAahMnKvV25Macfsy3TCWSCb9q-zomfqB0Eq3x0aaqzOvii1ZUkQgjai65Sl_-g6jL24HWK1vl6_5eArQKdUynZOjVmf4C8KErU0TK1GquqserJWHXsmK-iUuG4s_nU8X9UvwEEXKV_</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2638494333</pqid></control><display><type>article</type><title>Molecular characterization and functional analysis of daf-8 in the pinewood nematode, Bursaphelenchus xylophilus</title><source>SpringerLink Journals</source><source>Alma/SFX Local Collection</source><creator>Wang, Jinghan ; Hong, Huan ; Xie, Rong ; Ji, Jingjing ; Guo, Kai ; Bai, Liqun ; Tang, Jia ; Yu, Hongshi ; Ye, Jianren ; Hu, Jiafu</creator><creatorcontrib>Wang, Jinghan ; Hong, Huan ; Xie, Rong ; Ji, Jingjing ; Guo, Kai ; Bai, Liqun ; Tang, Jia ; Yu, Hongshi ; Ye, Jianren ; Hu, Jiafu</creatorcontrib><description>Bursaphelenchus xylophilus
, causal agent of pine wilt disease, causes extensive damage worldwide. Strategies are needed to inhibit population growth or block the spread of the invasive nematode to control pine wilt disease. The gene
daf-8
plays crucial roles in larval development and dauer formation in
Caenorhabditis elegans
, but little is known about its orthologue in
B. xylophilus
. In the present molecular characterization and functional analysis of
daf-8
in
B. xylophilus
(
Bx-daf-8
), RT-qPCR showed that the expression of
Bx-daf-8
gradually increased during the embryonic stage, peaked in the second-stage juvenile (J2), then dramatically dropped in the J3, and remained at that low level for the rest of its life.
Bx-daf-8-
transgenic
C. elegans
was employed to mimic the spatiotemporal expression of
Bx-daf-8
, which was expressed close to the pharynx during early embryogenesis and weakly throughout the whole body during late embryogenesis. It was observed in head neurons and tail ganglions throughout all postembryonic stages.
B. xylophilus
embryos were severely abnormal, and hatching rate decreased sharply after
Bx-daf-8
knockdown.
daf-16-1
and
da-f16-2
, downstream genes in the IIS pathway, also dropped sharply after
Bx-daf-8
knockdown. We propose that TGFβ may crosstalk with the IIS pathway upstream of
Bx-daf-16
and that
daf-8
may act as a master regulator of
daf-16
in
B. xylophilus
. However, knockdown of
Bx-daf-8
did not lead to constitutive developmental arrest at the dauer larval stage, indicating that dauer entry in
B. xylophilus
might be controlled by several genes and is more complicated than in
C. elegans
.
Bx-daf-8
alone did not control the dauer entry in
B. xylophilus
, but it was indispensable for embryogenesis, providing a potential target for suppressing population growth of
B. xylophilus
.</description><identifier>ISSN: 1007-662X</identifier><identifier>EISSN: 1993-0607</identifier><identifier>DOI: 10.1007/s11676-021-01335-7</identifier><language>eng</language><publisher>Singapore: Springer Nature Singapore</publisher><subject>Analysis ; Biomedical and Life Sciences ; Bursaphelenchus xylophilus ; Crosstalk ; Developmental stages ; Disease control ; Embryogenesis ; Embryonic growth stage ; Forestry ; Functional analysis ; Genes ; Genetic engineering ; Hatching ; Larval development ; Life Sciences ; Low level ; Nematoda ; Nematodes ; Original Paper ; Pharynx ; Population growth ; Transforming growth factors ; Wilt</subject><ispartof>Journal of forestry research, 2022-04, Vol.33 (2), p.689-698</ispartof><rights>Northeast Forestry University 2021. corrected publication 2022</rights><rights>COPYRIGHT 2022 Springer</rights><rights>Northeast Forestry University 2021. corrected publication 2022.</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c416t-19325a972d270d01dfdb65c0242ce8187be81473e3fe765411cb1d795dcadfa43</citedby><cites>FETCH-LOGICAL-c416t-19325a972d270d01dfdb65c0242ce8187be81473e3fe765411cb1d795dcadfa43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/lyyj/lyyj.jpg</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11676-021-01335-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11676-021-01335-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids></links><search><creatorcontrib>Wang, Jinghan</creatorcontrib><creatorcontrib>Hong, Huan</creatorcontrib><creatorcontrib>Xie, Rong</creatorcontrib><creatorcontrib>Ji, Jingjing</creatorcontrib><creatorcontrib>Guo, Kai</creatorcontrib><creatorcontrib>Bai, Liqun</creatorcontrib><creatorcontrib>Tang, Jia</creatorcontrib><creatorcontrib>Yu, Hongshi</creatorcontrib><creatorcontrib>Ye, Jianren</creatorcontrib><creatorcontrib>Hu, Jiafu</creatorcontrib><title>Molecular characterization and functional analysis of daf-8 in the pinewood nematode, Bursaphelenchus xylophilus</title><title>Journal of forestry research</title><addtitle>J. For. Res</addtitle><description>Bursaphelenchus xylophilus
, causal agent of pine wilt disease, causes extensive damage worldwide. Strategies are needed to inhibit population growth or block the spread of the invasive nematode to control pine wilt disease. The gene
daf-8
plays crucial roles in larval development and dauer formation in
Caenorhabditis elegans
, but little is known about its orthologue in
B. xylophilus
. In the present molecular characterization and functional analysis of
daf-8
in
B. xylophilus
(
Bx-daf-8
), RT-qPCR showed that the expression of
Bx-daf-8
gradually increased during the embryonic stage, peaked in the second-stage juvenile (J2), then dramatically dropped in the J3, and remained at that low level for the rest of its life.
Bx-daf-8-
transgenic
C. elegans
was employed to mimic the spatiotemporal expression of
Bx-daf-8
, which was expressed close to the pharynx during early embryogenesis and weakly throughout the whole body during late embryogenesis. It was observed in head neurons and tail ganglions throughout all postembryonic stages.
B. xylophilus
embryos were severely abnormal, and hatching rate decreased sharply after
Bx-daf-8
knockdown.
daf-16-1
and
da-f16-2
, downstream genes in the IIS pathway, also dropped sharply after
Bx-daf-8
knockdown. We propose that TGFβ may crosstalk with the IIS pathway upstream of
Bx-daf-16
and that
daf-8
may act as a master regulator of
daf-16
in
B. xylophilus
. However, knockdown of
Bx-daf-8
did not lead to constitutive developmental arrest at the dauer larval stage, indicating that dauer entry in
B. xylophilus
might be controlled by several genes and is more complicated than in
C. elegans
.
Bx-daf-8
alone did not control the dauer entry in
B. xylophilus
, but it was indispensable for embryogenesis, providing a potential target for suppressing population growth of
B. xylophilus
.</description><subject>Analysis</subject><subject>Biomedical and Life Sciences</subject><subject>Bursaphelenchus xylophilus</subject><subject>Crosstalk</subject><subject>Developmental stages</subject><subject>Disease control</subject><subject>Embryogenesis</subject><subject>Embryonic growth stage</subject><subject>Forestry</subject><subject>Functional analysis</subject><subject>Genes</subject><subject>Genetic engineering</subject><subject>Hatching</subject><subject>Larval development</subject><subject>Life Sciences</subject><subject>Low level</subject><subject>Nematoda</subject><subject>Nematodes</subject><subject>Original Paper</subject><subject>Pharynx</subject><subject>Population growth</subject><subject>Transforming growth factors</subject><subject>Wilt</subject><issn>1007-662X</issn><issn>1993-0607</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9kV1rFDEUhgexYK3-Aa8C4p2p-ZhJdi5rUStUvGmhd-FsPnayZJMxmaGOv95sR1gEkRySc8LzhpPzNs0bSi4pIfJDoVRIgQmjmFDOOyyfNee07zkmgsjnNa8UFoI9vGhelrInpGs5b8-b8VsKVs8BMtIDZNCTzf4XTD5FBNEgN0d9LCDUEsJSfEHJIQMOb5CPaBosGn20jykZFO0BpmTse_RxzgXGwQYb9TAX9HMJaRx8mMur5sxBKPb1n_Oiuf_86e76Bt9-__L1-uoW65aKCdOesw56yQyTxBBqnNmKThPWMm03dCO3dW8lt9xZKbqWUr2lRvad0WActPyiebe--wjRQdypfZpz_UFRYVn2jLAahMnKvV25Macfsy3TCWSCb9q-zomfqB0Eq3x0aaqzOvii1ZUkQgjai65Sl_-g6jL24HWK1vl6_5eArQKdUynZOjVmf4C8KErU0TK1GquqserJWHXsmK-iUuG4s_nU8X9UvwEEXKV_</recordid><startdate>20220401</startdate><enddate>20220401</enddate><creator>Wang, Jinghan</creator><creator>Hong, Huan</creator><creator>Xie, Rong</creator><creator>Ji, Jingjing</creator><creator>Guo, Kai</creator><creator>Bai, Liqun</creator><creator>Tang, Jia</creator><creator>Yu, Hongshi</creator><creator>Ye, Jianren</creator><creator>Hu, Jiafu</creator><general>Springer Nature Singapore</general><general>Springer</general><general>Springer Nature B.V</general><general>Nanjing Forestry University,Nanjing 210037,China</general><general>Zhejiang Agricultural and Forestry University, Hangzhou 311300,China%Zhejiang Agricultural and Forestry University, Hangzhou 311300,China%Yuhuan Natural Resources and Planning Bureau, Taizhou 317600,China%Zhejiang Agricultural and Forestry University, Hangzhou 311300,China</general><general>School of BioSciences,The University of Melbourne, Parkville,VIC 3010,Australia%Nanjing Forestry University,Nanjing 210037,China</general><scope>AAYXX</scope><scope>CITATION</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20220401</creationdate><title>Molecular characterization and functional analysis of daf-8 in the pinewood nematode, Bursaphelenchus xylophilus</title><author>Wang, Jinghan ; Hong, Huan ; Xie, Rong ; Ji, Jingjing ; Guo, Kai ; Bai, Liqun ; Tang, Jia ; Yu, Hongshi ; Ye, Jianren ; Hu, Jiafu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c416t-19325a972d270d01dfdb65c0242ce8187be81473e3fe765411cb1d795dcadfa43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Analysis</topic><topic>Biomedical and Life Sciences</topic><topic>Bursaphelenchus xylophilus</topic><topic>Crosstalk</topic><topic>Developmental stages</topic><topic>Disease control</topic><topic>Embryogenesis</topic><topic>Embryonic growth stage</topic><topic>Forestry</topic><topic>Functional analysis</topic><topic>Genes</topic><topic>Genetic engineering</topic><topic>Hatching</topic><topic>Larval development</topic><topic>Life Sciences</topic><topic>Low level</topic><topic>Nematoda</topic><topic>Nematodes</topic><topic>Original Paper</topic><topic>Pharynx</topic><topic>Population growth</topic><topic>Transforming growth factors</topic><topic>Wilt</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Jinghan</creatorcontrib><creatorcontrib>Hong, Huan</creatorcontrib><creatorcontrib>Xie, Rong</creatorcontrib><creatorcontrib>Ji, Jingjing</creatorcontrib><creatorcontrib>Guo, Kai</creatorcontrib><creatorcontrib>Bai, Liqun</creatorcontrib><creatorcontrib>Tang, Jia</creatorcontrib><creatorcontrib>Yu, Hongshi</creatorcontrib><creatorcontrib>Ye, Jianren</creatorcontrib><creatorcontrib>Hu, Jiafu</creatorcontrib><collection>CrossRef</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Journal of forestry research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Jinghan</au><au>Hong, Huan</au><au>Xie, Rong</au><au>Ji, Jingjing</au><au>Guo, Kai</au><au>Bai, Liqun</au><au>Tang, Jia</au><au>Yu, Hongshi</au><au>Ye, Jianren</au><au>Hu, Jiafu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterization and functional analysis of daf-8 in the pinewood nematode, Bursaphelenchus xylophilus</atitle><jtitle>Journal of forestry research</jtitle><stitle>J. For. Res</stitle><date>2022-04-01</date><risdate>2022</risdate><volume>33</volume><issue>2</issue><spage>689</spage><epage>698</epage><pages>689-698</pages><issn>1007-662X</issn><eissn>1993-0607</eissn><abstract>Bursaphelenchus xylophilus
, causal agent of pine wilt disease, causes extensive damage worldwide. Strategies are needed to inhibit population growth or block the spread of the invasive nematode to control pine wilt disease. The gene
daf-8
plays crucial roles in larval development and dauer formation in
Caenorhabditis elegans
, but little is known about its orthologue in
B. xylophilus
. In the present molecular characterization and functional analysis of
daf-8
in
B. xylophilus
(
Bx-daf-8
), RT-qPCR showed that the expression of
Bx-daf-8
gradually increased during the embryonic stage, peaked in the second-stage juvenile (J2), then dramatically dropped in the J3, and remained at that low level for the rest of its life.
Bx-daf-8-
transgenic
C. elegans
was employed to mimic the spatiotemporal expression of
Bx-daf-8
, which was expressed close to the pharynx during early embryogenesis and weakly throughout the whole body during late embryogenesis. It was observed in head neurons and tail ganglions throughout all postembryonic stages.
B. xylophilus
embryos were severely abnormal, and hatching rate decreased sharply after
Bx-daf-8
knockdown.
daf-16-1
and
da-f16-2
, downstream genes in the IIS pathway, also dropped sharply after
Bx-daf-8
knockdown. We propose that TGFβ may crosstalk with the IIS pathway upstream of
Bx-daf-16
and that
daf-8
may act as a master regulator of
daf-16
in
B. xylophilus
. However, knockdown of
Bx-daf-8
did not lead to constitutive developmental arrest at the dauer larval stage, indicating that dauer entry in
B. xylophilus
might be controlled by several genes and is more complicated than in
C. elegans
.
Bx-daf-8
alone did not control the dauer entry in
B. xylophilus
, but it was indispensable for embryogenesis, providing a potential target for suppressing population growth of
B. xylophilus
.</abstract><cop>Singapore</cop><pub>Springer Nature Singapore</pub><doi>10.1007/s11676-021-01335-7</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1007-662X |
| ispartof | Journal of forestry research, 2022-04, Vol.33 (2), p.689-698 |
| issn | 1007-662X 1993-0607 |
| language | eng |
| recordid | cdi_wanfang_journals_lyyj202202027 |
| source | SpringerLink Journals; Alma/SFX Local Collection |
| subjects | Analysis Biomedical and Life Sciences Bursaphelenchus xylophilus Crosstalk Developmental stages Disease control Embryogenesis Embryonic growth stage Forestry Functional analysis Genes Genetic engineering Hatching Larval development Life Sciences Low level Nematoda Nematodes Original Paper Pharynx Population growth Transforming growth factors Wilt |
| title | Molecular characterization and functional analysis of daf-8 in the pinewood nematode, Bursaphelenchus xylophilus |
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