An efficient in vitro shoot regeneration through direct organogenesis from seedling-derived petiole and leaf segments and acclimatization of Ficus religiosa
Prolific and rapid in vitro plant organogenesis via direct regeneration has been obtained from axenic seedling-derived petiole and leaf explants of Ficus religiosa in Murashige and Skoog (MS) medium containing different concentrations of cytokinins in combination with indole-3-butyric acid (IBA). MS...
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| Veröffentlicht in: | Journal of forestry research 2019-06, Vol.30 (3), p.807-815 |
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| creator | Hesami, Mohsen Daneshvar, Mohammad Hosein Yoosefzadeh-Najafabadi, Mohsen |
| description | Prolific and rapid in vitro plant organogenesis via direct regeneration has been obtained from axenic seedling-derived petiole and leaf explants of
Ficus religiosa
in Murashige and Skoog (MS) medium containing different concentrations of cytokinins in combination with indole-3-butyric acid (IBA). MS medium with 1.5 mg/l 6-benzylaminopurine plus 0.15 mg/l IBA produced the highest shoot induction frequency with an average of 6.26 and 10.13 shoots per leaf and petiole explants, respectively. After 4 weeks, the highest root formation frequency (96.7%), root number (5.73), and root length (4.76 cm) were with MS medium containing 2.0 mg/l IBA plus 0.1 mg/l α-naphthalene acetic acid. In addition, the effect of four sodium nitroprusside (SNP) treatments on acclimatization was also studied. Highest morphological traits such as survival rates, fresh and dry root weights as well as antioxidant enzymatic activities such as superoxide dismutase, peroxidase, and catalase was achieved with 125 ppm SNP. The α-amino acid, proline, content was highest with this treatment while the highest H
2
O
2
(hydrogen peroxide) was in the controls. This study introduces a cost-effective, prolific, and efficient in vitro multiplication system to supply pharmaceutical and ornamental needs. It is the first report of an in vitro organogenesis protocol for
F. religiosa
by direct regeneration through axenic seedling-derived petiole and leaf explants, which can be efficiently employed for the utilization of active biomolecules. |
| doi_str_mv | 10.1007/s11676-018-0647-0 |
| format | Article |
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Ficus religiosa
in Murashige and Skoog (MS) medium containing different concentrations of cytokinins in combination with indole-3-butyric acid (IBA). MS medium with 1.5 mg/l 6-benzylaminopurine plus 0.15 mg/l IBA produced the highest shoot induction frequency with an average of 6.26 and 10.13 shoots per leaf and petiole explants, respectively. After 4 weeks, the highest root formation frequency (96.7%), root number (5.73), and root length (4.76 cm) were with MS medium containing 2.0 mg/l IBA plus 0.1 mg/l α-naphthalene acetic acid. In addition, the effect of four sodium nitroprusside (SNP) treatments on acclimatization was also studied. Highest morphological traits such as survival rates, fresh and dry root weights as well as antioxidant enzymatic activities such as superoxide dismutase, peroxidase, and catalase was achieved with 125 ppm SNP. The α-amino acid, proline, content was highest with this treatment while the highest H
2
O
2
(hydrogen peroxide) was in the controls. This study introduces a cost-effective, prolific, and efficient in vitro multiplication system to supply pharmaceutical and ornamental needs. It is the first report of an in vitro organogenesis protocol for
F. religiosa
by direct regeneration through axenic seedling-derived petiole and leaf explants, which can be efficiently employed for the utilization of active biomolecules.</description><identifier>ISSN: 1007-662X</identifier><identifier>EISSN: 1993-0607</identifier><identifier>DOI: 10.1007/s11676-018-0647-0</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Acclimatization ; Acetic acid ; Amino acids ; Antioxidants ; Auxin ; Biomedical and Life Sciences ; Biomolecules ; Butyric acid ; Catalase ; Cytokinins ; Enzymatic activity ; Enzymes ; Ethylenediaminetetraacetic acid ; Explants ; Ficus religiosa ; Forestry ; Hydrogen peroxide ; Indole-3-butyric acid ; Leaves ; Life Sciences ; Multiplication ; Naphthalene ; Organogenesis ; Original Paper ; Peroxidase ; Proline ; Regeneration ; Seedlings ; Shoots ; Sodium ; Sodium nitroprusside ; Superoxide dismutase</subject><ispartof>Journal of forestry research, 2019-06, Vol.30 (3), p.807-815</ispartof><rights>Northeast Forestry University and Springer-Verlag GmbH Germany, part of Springer Nature 2018</rights><rights>COPYRIGHT 2019 Springer</rights><rights>Copyright Springer Nature B.V. 2019</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-e1b72d15277afc036069987dbbd3a0ef562a1e83cd7c0fbde477064ed9d463ea3</citedby><cites>FETCH-LOGICAL-c413t-e1b72d15277afc036069987dbbd3a0ef562a1e83cd7c0fbde477064ed9d463ea3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/lyyj/lyyj.jpg</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11676-018-0647-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11676-018-0647-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Hesami, Mohsen</creatorcontrib><creatorcontrib>Daneshvar, Mohammad Hosein</creatorcontrib><creatorcontrib>Yoosefzadeh-Najafabadi, Mohsen</creatorcontrib><title>An efficient in vitro shoot regeneration through direct organogenesis from seedling-derived petiole and leaf segments and acclimatization of Ficus religiosa</title><title>Journal of forestry research</title><addtitle>J. For. Res</addtitle><description>Prolific and rapid in vitro plant organogenesis via direct regeneration has been obtained from axenic seedling-derived petiole and leaf explants of
Ficus religiosa
in Murashige and Skoog (MS) medium containing different concentrations of cytokinins in combination with indole-3-butyric acid (IBA). MS medium with 1.5 mg/l 6-benzylaminopurine plus 0.15 mg/l IBA produced the highest shoot induction frequency with an average of 6.26 and 10.13 shoots per leaf and petiole explants, respectively. After 4 weeks, the highest root formation frequency (96.7%), root number (5.73), and root length (4.76 cm) were with MS medium containing 2.0 mg/l IBA plus 0.1 mg/l α-naphthalene acetic acid. In addition, the effect of four sodium nitroprusside (SNP) treatments on acclimatization was also studied. Highest morphological traits such as survival rates, fresh and dry root weights as well as antioxidant enzymatic activities such as superoxide dismutase, peroxidase, and catalase was achieved with 125 ppm SNP. The α-amino acid, proline, content was highest with this treatment while the highest H
2
O
2
(hydrogen peroxide) was in the controls. This study introduces a cost-effective, prolific, and efficient in vitro multiplication system to supply pharmaceutical and ornamental needs. It is the first report of an in vitro organogenesis protocol for
F. religiosa
by direct regeneration through axenic seedling-derived petiole and leaf explants, which can be efficiently employed for the utilization of active biomolecules.</description><subject>Acclimatization</subject><subject>Acetic acid</subject><subject>Amino acids</subject><subject>Antioxidants</subject><subject>Auxin</subject><subject>Biomedical and Life Sciences</subject><subject>Biomolecules</subject><subject>Butyric acid</subject><subject>Catalase</subject><subject>Cytokinins</subject><subject>Enzymatic activity</subject><subject>Enzymes</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Explants</subject><subject>Ficus religiosa</subject><subject>Forestry</subject><subject>Hydrogen peroxide</subject><subject>Indole-3-butyric acid</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Multiplication</subject><subject>Naphthalene</subject><subject>Organogenesis</subject><subject>Original Paper</subject><subject>Peroxidase</subject><subject>Proline</subject><subject>Regeneration</subject><subject>Seedlings</subject><subject>Shoots</subject><subject>Sodium</subject><subject>Sodium nitroprusside</subject><subject>Superoxide dismutase</subject><issn>1007-662X</issn><issn>1993-0607</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1ksGKFDEQhhtxYdddH2BvAfHYa9LpTqaPw-KuwoIXBW8hk1QyGbqTMcmsjM_iw1pjC4Og5JCk8v31F5VqmltG7xil8l1hTEjRUrZqqehlS180V2wcOd6ofIlnhFohuq-XzatSdpQOPef9VfNzHQk4F0yAWEmI5DnUnEjZplRJBg8Rsq4hRVK3OR38ltiQwVSSstcxnd5LKMTlNJMCYKcQfWshh2ewZA-onIDoaMkE2iHhZ_QpvyPamCnMmPzHYpAceQjmUNB2Cj6kom-aC6enAq__7NfNl4f3n-8_tE-fHj_er59a0zNeW2Ab2Vk2dFJqZygXVIzjStrNxnJNwQ2i0wxW3FhpqNtY6KXEJoEdbS84aH7dvF3yftfR6ejVLh1yREc1HY-7jrKRcuwfcm8Wbp_TtwOUega7jg2DWKH5mfJ6AhWiSzVrM4di1FoyKUeJpSJ19w8Kl4U5mBTBBYz_JWCLwORUSgan9hnbl4-KUXX6XrXMgMIZUKcZUBQ13aIpyEYP-Vzw_0W_AJGVtkw</recordid><startdate>20190601</startdate><enddate>20190601</enddate><creator>Hesami, Mohsen</creator><creator>Daneshvar, Mohammad Hosein</creator><creator>Yoosefzadeh-Najafabadi, Mohsen</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><general>Department of Horticulture Science, University of Tehran, Karaj, Alborz 14176-14418, Iran%Department of Horticulture Science, Ramin University of Agriculture and Natural Resources, Ahwaz, Khuzestan 63417-73637, Iran</general><scope>AAYXX</scope><scope>CITATION</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20190601</creationdate><title>An efficient in vitro shoot regeneration through direct organogenesis from seedling-derived petiole and leaf segments and acclimatization of Ficus religiosa</title><author>Hesami, Mohsen ; Daneshvar, Mohammad Hosein ; Yoosefzadeh-Najafabadi, Mohsen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-e1b72d15277afc036069987dbbd3a0ef562a1e83cd7c0fbde477064ed9d463ea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Acclimatization</topic><topic>Acetic acid</topic><topic>Amino acids</topic><topic>Antioxidants</topic><topic>Auxin</topic><topic>Biomedical and Life Sciences</topic><topic>Biomolecules</topic><topic>Butyric acid</topic><topic>Catalase</topic><topic>Cytokinins</topic><topic>Enzymatic activity</topic><topic>Enzymes</topic><topic>Ethylenediaminetetraacetic acid</topic><topic>Explants</topic><topic>Ficus religiosa</topic><topic>Forestry</topic><topic>Hydrogen peroxide</topic><topic>Indole-3-butyric acid</topic><topic>Leaves</topic><topic>Life Sciences</topic><topic>Multiplication</topic><topic>Naphthalene</topic><topic>Organogenesis</topic><topic>Original Paper</topic><topic>Peroxidase</topic><topic>Proline</topic><topic>Regeneration</topic><topic>Seedlings</topic><topic>Shoots</topic><topic>Sodium</topic><topic>Sodium nitroprusside</topic><topic>Superoxide dismutase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hesami, Mohsen</creatorcontrib><creatorcontrib>Daneshvar, Mohammad Hosein</creatorcontrib><creatorcontrib>Yoosefzadeh-Najafabadi, Mohsen</creatorcontrib><collection>CrossRef</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Journal of forestry research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hesami, Mohsen</au><au>Daneshvar, Mohammad Hosein</au><au>Yoosefzadeh-Najafabadi, Mohsen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An efficient in vitro shoot regeneration through direct organogenesis from seedling-derived petiole and leaf segments and acclimatization of Ficus religiosa</atitle><jtitle>Journal of forestry research</jtitle><stitle>J. For. Res</stitle><date>2019-06-01</date><risdate>2019</risdate><volume>30</volume><issue>3</issue><spage>807</spage><epage>815</epage><pages>807-815</pages><issn>1007-662X</issn><eissn>1993-0607</eissn><abstract>Prolific and rapid in vitro plant organogenesis via direct regeneration has been obtained from axenic seedling-derived petiole and leaf explants of
Ficus religiosa
in Murashige and Skoog (MS) medium containing different concentrations of cytokinins in combination with indole-3-butyric acid (IBA). MS medium with 1.5 mg/l 6-benzylaminopurine plus 0.15 mg/l IBA produced the highest shoot induction frequency with an average of 6.26 and 10.13 shoots per leaf and petiole explants, respectively. After 4 weeks, the highest root formation frequency (96.7%), root number (5.73), and root length (4.76 cm) were with MS medium containing 2.0 mg/l IBA plus 0.1 mg/l α-naphthalene acetic acid. In addition, the effect of four sodium nitroprusside (SNP) treatments on acclimatization was also studied. Highest morphological traits such as survival rates, fresh and dry root weights as well as antioxidant enzymatic activities such as superoxide dismutase, peroxidase, and catalase was achieved with 125 ppm SNP. The α-amino acid, proline, content was highest with this treatment while the highest H
2
O
2
(hydrogen peroxide) was in the controls. This study introduces a cost-effective, prolific, and efficient in vitro multiplication system to supply pharmaceutical and ornamental needs. It is the first report of an in vitro organogenesis protocol for
F. religiosa
by direct regeneration through axenic seedling-derived petiole and leaf explants, which can be efficiently employed for the utilization of active biomolecules.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s11676-018-0647-0</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1007-662X |
| ispartof | Journal of forestry research, 2019-06, Vol.30 (3), p.807-815 |
| issn | 1007-662X 1993-0607 |
| language | eng |
| recordid | cdi_wanfang_journals_lyyj201903007 |
| source | Alma/SFX Local Collection; SpringerLink Journals - AutoHoldings |
| subjects | Acclimatization Acetic acid Amino acids Antioxidants Auxin Biomedical and Life Sciences Biomolecules Butyric acid Catalase Cytokinins Enzymatic activity Enzymes Ethylenediaminetetraacetic acid Explants Ficus religiosa Forestry Hydrogen peroxide Indole-3-butyric acid Leaves Life Sciences Multiplication Naphthalene Organogenesis Original Paper Peroxidase Proline Regeneration Seedlings Shoots Sodium Sodium nitroprusside Superoxide dismutase |
| title | An efficient in vitro shoot regeneration through direct organogenesis from seedling-derived petiole and leaf segments and acclimatization of Ficus religiosa |
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