Micropropagation of yellow mangosteen: a valuable endemic tree of India
We developed a method for in vitro regenera- tion of Garcinia xanthochymus (yellow mangosteen) from matured seed segments. Multiple shoots were induced on woody plant (WP) medium supplemented with cytokinins. An average of 11 shoots per explant were regenerated from mature seed segments on WP medium...
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creator | Patil, Lalita Mahantagouda Murthy, Hosakatte Niranjana Dandin, Vijayalaxmi S Koli, Sunita P Manohar, Shirugumbi Hanamanthagouda Payamalle, Shivanand D |
description | We developed a method for in vitro regenera- tion of Garcinia xanthochymus (yellow mangosteen) from matured seed segments. Multiple shoots were induced on woody plant (WP) medium supplemented with cytokinins. An average of 11 shoots per explant were regenerated from mature seed segments on WP medium containing 20 μM 6-benzylaminopurine. Histological analysis revealed that hypodermal cells of seed segments were initially involved in active division, which later developed into meriste- moids, subsequently leading to the formation of shoot buds. Shoot elongation was achieved by repeated subculturing of seed explants in shoot regeneration medium. Rooting of shoots was achieved on WP medium supplemented with indole-3-butyric acid or s-naphthalene acetic acid. Plant- lets were transplanted to pots containing soil: compost (1:1) and survival rate was 90 %. |
doi_str_mv | 10.1007/s11676-015-0102-4 |
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Multiple shoots were induced on woody plant (WP) medium supplemented with cytokinins. An average of 11 shoots per explant were regenerated from mature seed segments on WP medium containing 20 μM 6-benzylaminopurine. Histological analysis revealed that hypodermal cells of seed segments were initially involved in active division, which later developed into meriste- moids, subsequently leading to the formation of shoot buds. Shoot elongation was achieved by repeated subculturing of seed explants in shoot regeneration medium. Rooting of shoots was achieved on WP medium supplemented with indole-3-butyric acid or s-naphthalene acetic acid. Plant- lets were transplanted to pots containing soil: compost (1:1) and survival rate was 90 %.</description><identifier>ISSN: 1007-662X</identifier><identifier>EISSN: 1993-0607</identifier><identifier>DOI: 10.1007/s11676-015-0102-4</identifier><language>eng</language><publisher>Harbin: Northeast Forestry University</publisher><subject>Acetic acid ; Analysis ; Auxin ; benzyladenine ; Biomedical and Life Sciences ; buds ; composts ; Cytokinins ; explants ; Forestry ; Garcinia xanthochymus ; in vitro regeneration ; indigenous species ; indole butyric acid ; Life Sciences ; mangosteens ; micropropagation ; naphthaleneacetic acid ; Organic acids ; Original Paper ; plantlets ; rooting ; shoots ; soil ; survival rate ; woody plants ; 体外再生 ; 印度 ; 山竹 ; 成熟种子 ; 木本植物 ; 细胞分裂素 ; 诱导培养基 ; 黄色</subject><ispartof>Journal of forestry research, 2016-02, Vol.27 (1), p.161-165</ispartof><rights>Northeast Forestry University and Springer-Verlag Berlin Heidelberg 2015</rights><rights>COPYRIGHT 2016 Springer</rights><rights>Copyright © Wanfang Data Co. 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For. Res</addtitle><addtitle>Journal of Forestry Research</addtitle><description>We developed a method for in vitro regenera- tion of Garcinia xanthochymus (yellow mangosteen) from matured seed segments. Multiple shoots were induced on woody plant (WP) medium supplemented with cytokinins. An average of 11 shoots per explant were regenerated from mature seed segments on WP medium containing 20 μM 6-benzylaminopurine. Histological analysis revealed that hypodermal cells of seed segments were initially involved in active division, which later developed into meriste- moids, subsequently leading to the formation of shoot buds. Shoot elongation was achieved by repeated subculturing of seed explants in shoot regeneration medium. Rooting of shoots was achieved on WP medium supplemented with indole-3-butyric acid or s-naphthalene acetic acid. Plant- lets were transplanted to pots containing soil: compost (1:1) and survival rate was 90 %.</description><subject>Acetic acid</subject><subject>Analysis</subject><subject>Auxin</subject><subject>benzyladenine</subject><subject>Biomedical and Life Sciences</subject><subject>buds</subject><subject>composts</subject><subject>Cytokinins</subject><subject>explants</subject><subject>Forestry</subject><subject>Garcinia xanthochymus</subject><subject>in vitro regeneration</subject><subject>indigenous species</subject><subject>indole butyric acid</subject><subject>Life Sciences</subject><subject>mangosteens</subject><subject>micropropagation</subject><subject>naphthaleneacetic acid</subject><subject>Organic acids</subject><subject>Original Paper</subject><subject>plantlets</subject><subject>rooting</subject><subject>shoots</subject><subject>soil</subject><subject>survival rate</subject><subject>woody plants</subject><subject>体外再生</subject><subject>印度</subject><subject>山竹</subject><subject>成熟种子</subject><subject>木本植物</subject><subject>细胞分裂素</subject><subject>诱导培养基</subject><subject>黄色</subject><issn>1007-662X</issn><issn>1993-0607</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNp9kcFO3DAQhqOqlUqhD9BTIyqOgRkncdZHhEpZCcShRerNmiR26lXWBjsU7avwLLxTX4FZgkCVqsq2xrK-f_zPTJZ9QjhEgOYoIcpGFoA1HxBF9SbbQaXKAiQ0b_nOUCGl-Pk--5DSCqCuyrLayZYXrovhmjcNNLng82DzjRnHcJevyQ8hTcb4Pw_3OeW_abyldjS58b1Zuy6fojFbful7R3vZO0tjMh-f4252dfr1x8lZcX75bXlyfF50NcipsKJW1Pc9G-ikQBRt17eqVXVrse9baVCREcpK6hjAUoi6plbZkuSirhDL3exgzntH3rJDvQq30fOPetxsVgJQcgOwYe7LzA00Gu28DVOkbu1Sp48bbBYAi0oydfgPitdThcEb6_j9LwHOAm5bStFYfR3dmuJGI-htl_U8Cs2j0NtR6Io1YtYkZv1g4qvn_4k-zyJLQdMQXdJX35_KA1AC5NbK_rOVX8EPN5z5xYuUjUKmoHwEHUSf2g</recordid><startdate>20160201</startdate><enddate>20160201</enddate><creator>Patil, Lalita Mahantagouda</creator><creator>Murthy, Hosakatte Niranjana</creator><creator>Dandin, Vijayalaxmi S</creator><creator>Koli, Sunita P</creator><creator>Manohar, Shirugumbi Hanamanthagouda</creator><creator>Payamalle, Shivanand D</creator><general>Northeast Forestry University</general><general>Springer</general><general>Department of Botany,Karnatak University,Dharwad 580 003,India</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W95</scope><scope>~WA</scope><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20160201</creationdate><title>Micropropagation of yellow mangosteen: a valuable endemic tree of India</title><author>Patil, Lalita Mahantagouda ; 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For. Res</stitle><addtitle>Journal of Forestry Research</addtitle><date>2016-02-01</date><risdate>2016</risdate><volume>27</volume><issue>1</issue><spage>161</spage><epage>165</epage><pages>161-165</pages><issn>1007-662X</issn><eissn>1993-0607</eissn><abstract>We developed a method for in vitro regenera- tion of Garcinia xanthochymus (yellow mangosteen) from matured seed segments. Multiple shoots were induced on woody plant (WP) medium supplemented with cytokinins. An average of 11 shoots per explant were regenerated from mature seed segments on WP medium containing 20 μM 6-benzylaminopurine. Histological analysis revealed that hypodermal cells of seed segments were initially involved in active division, which later developed into meriste- moids, subsequently leading to the formation of shoot buds. Shoot elongation was achieved by repeated subculturing of seed explants in shoot regeneration medium. Rooting of shoots was achieved on WP medium supplemented with indole-3-butyric acid or s-naphthalene acetic acid. Plant- lets were transplanted to pots containing soil: compost (1:1) and survival rate was 90 %.</abstract><cop>Harbin</cop><pub>Northeast Forestry University</pub><doi>10.1007/s11676-015-0102-4</doi><tpages>5</tpages></addata></record> |
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source | Alma/SFX Local Collection; SpringerLink Journals - AutoHoldings |
subjects | Acetic acid Analysis Auxin benzyladenine Biomedical and Life Sciences buds composts Cytokinins explants Forestry Garcinia xanthochymus in vitro regeneration indigenous species indole butyric acid Life Sciences mangosteens micropropagation naphthaleneacetic acid Organic acids Original Paper plantlets rooting shoots soil survival rate woody plants 体外再生 印度 山竹 成熟种子 木本植物 细胞分裂素 诱导培养基 黄色 |
title | Micropropagation of yellow mangosteen: a valuable endemic tree of India |
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