Analysis of bacterial community in bulking sludge using culture-dependent and -independent approaches
The bacterial community of a bulking sludge from a municipal wastewater treatment plant with anoxic-anaerobic-oxic process was investigated by combination of cultivation and 16S rRNA gene clone library analysis for understanding the causes of bulking.A total of 28 species were obtained from 63 isola...
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| Veröffentlicht in: | Journal of environmental sciences (China) 2011-01, Vol.23 (11), p.1880-1887 |
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| container_title | Journal of environmental sciences (China) |
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| creator | Jin, Decai Wang, Ping Bai, Zhihui Wang, Xinxin Peng, Hong Qi, Rong Yu, Zhisheng Zhuang, Guoqiang |
| description | The bacterial community of a bulking sludge from a municipal wastewater treatment plant with anoxic-anaerobic-oxic process was investigated by combination of cultivation and 16S rRNA gene clone library analysis for understanding the causes of bulking.A total of 28 species were obtained from 63 isolates collected from six culture media.The most cultivable species belonged to γ-Proteobacteria including Klebsiella sp.,Pseudomonas sp.,Aeromonas sp.and Acinetobacter sp.Further analysis of these strains by repetitive sequence based on polymerase chain reaction （rep-PCR） technology showed that rep-PCR yielded discriminatory banding patterns within the same genus using REP and BOX primer sets.While the culture-independent assessment revealed that β-Proteobacteria was the dominant group in the bulking sample.Sequence analysis revealed that the highest proportion （14.7%） of operational taxonomic units was 98% similar to Candidatus Accumulibacter phosphatis,which is used to remove phosphorous from wastewater.Our results indicated that combining different approaches can produce complementary information,thus generate a more accurate view of microbial community in bulking sludge. |
| doi_str_mv | 10.1016/S1001-0742(10)60621-3 |
| format | Article |
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All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c477t-4c4ee1b4e9bd71fda14378eb574ec28ebb4ebcf004ce57fbf6c8ebef86dd76093</citedby><cites>FETCH-LOGICAL-c477t-4c4ee1b4e9bd71fda14378eb574ec28ebb4ebcf004ce57fbf6c8ebef86dd76093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/85265X/85265X.jpg</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S1001-0742(10)60621-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22432314$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jin, Decai</creatorcontrib><creatorcontrib>Wang, Ping</creatorcontrib><creatorcontrib>Bai, Zhihui</creatorcontrib><creatorcontrib>Wang, Xinxin</creatorcontrib><creatorcontrib>Peng, Hong</creatorcontrib><creatorcontrib>Qi, Rong</creatorcontrib><creatorcontrib>Yu, Zhisheng</creatorcontrib><creatorcontrib>Zhuang, Guoqiang</creatorcontrib><title>Analysis of bacterial community in bulking sludge using culture-dependent and -independent approaches</title><title>Journal of environmental sciences (China)</title><addtitle>Journal of Environmental Sciences</addtitle><description>The bacterial community of a bulking sludge from a municipal wastewater treatment plant with anoxic-anaerobic-oxic process was investigated by combination of cultivation and 16S rRNA gene clone library analysis for understanding the causes of bulking.A total of 28 species were obtained from 63 isolates collected from six culture media.The most cultivable species belonged to γ-Proteobacteria including Klebsiella sp.,Pseudomonas sp.,Aeromonas sp.and Acinetobacter sp.Further analysis of these strains by repetitive sequence based on polymerase chain reaction （rep-PCR） technology showed that rep-PCR yielded discriminatory banding patterns within the same genus using REP and BOX primer sets.While the culture-independent assessment revealed that β-Proteobacteria was the dominant group in the bulking sample.Sequence analysis revealed that the highest proportion （14.7%） of operational taxonomic units was 98% similar to Candidatus Accumulibacter phosphatis,which is used to remove phosphorous from wastewater.Our results indicated that combining different approaches can produce complementary information,thus generate a more accurate view of microbial community in bulking sludge.</description><subject>16S rRNA gene clone library</subject><subject>Acinetobacter</subject><subject>Aeromonas</subject><subject>Bacteria - classification</subject><subject>Bacteria - genetics</subject><subject>Bacteria - growth & development</subject><subject>bacterial communities</subject><subject>Banding</subject><subject>beta-Proteobacteria</subject><subject>Biodiversity</subject><subject>bulking sludge</subject><subject>cultivable bacteria</subject><subject>culture media</subject><subject>Culture Techniques</subject><subject>DNA Fingerprinting</subject><subject>DNA primers</subject><subject>Gene Library</subject><subject>genes</subject><subject>Genes, Bacterial</subject><subject>Genes, rRNA</subject><subject>Klebsiella</subject><subject>Microbial Consortia</subject><subject>municipal wastewater</subject><subject>Polymerase Chain Reaction</subject><subject>Pseudomonas</subject><subject>REP</subject><subject>rep-PCR</subject><subject>ribosomal RNA</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>rRNA基因</subject><subject>sequence analysis</subject><subject>Sewage - microbiology</subject><subject>sludge</subject><subject>wastewater treatment</subject><subject>城市污水处理厂</subject><subject>微生物群落</subject><subject>文化</subject><subject>污泥膨胀</subject><subject>细菌群落</subject><subject>聚合酶链反应</subject><issn>1001-0742</issn><issn>1878-7320</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctu1TAQhiMEohd4BCBsCiwC40viZIWqiptUiUXp2nLscepDjnNqx6Dz9jjNacWu3ng8_mb8j_-ieEXgIwHSfLoiAKQCwel7Ah8aaCip2JPimLSirQSj8DTH98hRcRLjBgB4DfXz4ohSzigj_LjAc6_GfXSxnGzZKz1jcGos9bTdJu_mfel82afxt_NDGcdkBixTXA46jXMKWBncoTfo51J5U1bO_5fY7cKk9A3GF8Uzq8aILw_7aXH99cuvi-_V5c9vPy7OLyvNhZgrrjki6Tl2vRHEGkU4Ey32teCoaQ7yVa9tHkNjLWxvG52TaNvGGNFAx06Ls7XvX-Wt8oPcTCnkAaPcYJRIgeQFRGTw3QpmhbcJ4yy3LmocR-VxSlF2jFPBBW0fJ0kLvG15ncl6JXWYYgxo5S64rQp7SUAunsk7z-RiyJK680yyXPf68ELqt2gequ5NysCbFbBqkmoILsrrqzxLDUBJS5ulxeeVwPy5fxwGGbVDr9G4gHqWZnKPinh7EH8z-eE2O_ygg3UdZ4uUf9cau_U</recordid><startdate>20110101</startdate><enddate>20110101</enddate><creator>Jin, Decai</creator><creator>Wang, Ping</creator><creator>Bai, Zhihui</creator><creator>Wang, Xinxin</creator><creator>Peng, Hong</creator><creator>Qi, Rong</creator><creator>Yu, Zhisheng</creator><creator>Zhuang, Guoqiang</creator><general>Elsevier B.V</general><general>Graduate University of Chinese Academy of Sciences, Beijing 100049, China%Graduate University of Chinese Academy of Sciences, Beijing 100049, China%Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.%School of Chemical Engineering, The University of Queensland, Brisbane, Queensland 4072, Australia</general><general>Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W92</scope><scope>~WA</scope><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7ST</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20110101</creationdate><title>Analysis of bacterial community in bulking sludge using culture-dependent and -independent approaches</title><author>Jin, Decai ; Wang, Ping ; Bai, Zhihui ; Wang, Xinxin ; Peng, Hong ; Qi, Rong ; Yu, Zhisheng ; Zhuang, Guoqiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c477t-4c4ee1b4e9bd71fda14378eb574ec28ebb4ebcf004ce57fbf6c8ebef86dd76093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>16S rRNA gene clone library</topic><topic>Acinetobacter</topic><topic>Aeromonas</topic><topic>Bacteria - 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| subjects | 16S rRNA gene clone library Acinetobacter Aeromonas Bacteria - classification Bacteria - genetics Bacteria - growth & development bacterial communities Banding beta-Proteobacteria Biodiversity bulking sludge cultivable bacteria culture media Culture Techniques DNA Fingerprinting DNA primers Gene Library genes Genes, Bacterial Genes, rRNA Klebsiella Microbial Consortia municipal wastewater Polymerase Chain Reaction Pseudomonas REP rep-PCR ribosomal RNA RNA, Ribosomal, 16S - genetics rRNA基因 sequence analysis Sewage - microbiology sludge wastewater treatment 城市污水处理厂 微生物群落 文化 污泥膨胀 细菌群落 聚合酶链反应 |
| title | Analysis of bacterial community in bulking sludge using culture-dependent and -independent approaches |
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