Analysis of phosphate-accumulating organisms cultivated under different carbon sources with polymerase chain reaction-denaturing gradient gel electrophoresis assay
To investigate the microbial communities of microorganisms cultivated under different carbon sources, three sequencing batch reactors were operated. They were supplied with sewage, glucose and sodium acetate as carbon sources respectively and showed high phosphorus removal performance. The results o...
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| Veröffentlicht in: | Journal of environmental sciences (China) 2005, Vol.17 (4), p.611-614 |
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| container_title | Journal of environmental sciences (China) |
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| creator | Yu, Shui-Li Liu, Ya-Nan Jing, Guo-Lin Zhao, Bing-Jie Guo, Si-Yuan |
| description | To investigate the microbial communities of microorganisms cultivated under different carbon sources, three sequencing batch reactors were operated. They were supplied with sewage, glucose and sodium acetate as carbon sources respectively and showed high phosphorus removal performance. The results of denaturing gradient gel electrophoresis(DGGE) of polymerase chain reaction-amplified (PCR) 16S rDNA fragments demonstrated that β-protebacteria, Actinomyces sp. and y-protebactefia only exited in 1# reactor. The microbiological diversity of 1# reactor exceeded the other two reactors. Flavobacterium, Bacillales, Actinomyces, Actinobacteridae and uncultured bacteria(AF527584, AF502204, AY592749, AB076862, AJ619051, AF495454 and AY133070) could be detected in the biological phosphorus removal reactors. |
| doi_str_mv | 10.3321/j.issn:1001-0742.2005.04.017 |
| format | Article |
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They were supplied with sewage, glucose and sodium acetate as carbon sources respectively and showed high phosphorus removal performance. The results of denaturing gradient gel electrophoresis(DGGE) of polymerase chain reaction-amplified (PCR) 16S rDNA fragments demonstrated that β-protebacteria, Actinomyces sp. and y-protebactefia only exited in 1# reactor. The microbiological diversity of 1# reactor exceeded the other two reactors. Flavobacterium, Bacillales, Actinomyces, Actinobacteridae and uncultured bacteria(AF527584, AF502204, AY592749, AB076862, AJ619051, AF495454 and AY133070) could be detected in the biological phosphorus removal reactors.</description><identifier>ISSN: 1001-0742</identifier><identifier>EISSN: 1878-7320</identifier><identifier>DOI: 10.3321/j.issn:1001-0742.2005.04.017</identifier><identifier>PMID: 16158589</identifier><language>eng</language><publisher>Netherlands: School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China</publisher><subject>16S ; Bacteria - metabolism ; Base Sequence ; Carbon - metabolism ; DNA Primers ; Electrophoresis - methods ; Molecular Sequence Data ; PCR-DGGE ; Phosphates - metabolism ; Polymerase Chain Reaction - methods ; rDNA ; 生物磷迁移 ; 移动反应</subject><ispartof>Journal of environmental sciences (China), 2005, Vol.17 (4), p.611-614</ispartof><rights>Copyright © Wanfang Data Co. Ltd. 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They were supplied with sewage, glucose and sodium acetate as carbon sources respectively and showed high phosphorus removal performance. The results of denaturing gradient gel electrophoresis(DGGE) of polymerase chain reaction-amplified (PCR) 16S rDNA fragments demonstrated that β-protebacteria, Actinomyces sp. and y-protebactefia only exited in 1# reactor. The microbiological diversity of 1# reactor exceeded the other two reactors. Flavobacterium, Bacillales, Actinomyces, Actinobacteridae and uncultured bacteria(AF527584, AF502204, AY592749, AB076862, AJ619051, AF495454 and AY133070) could be detected in the biological phosphorus removal reactors.</description><subject>16S</subject><subject>Bacteria - metabolism</subject><subject>Base Sequence</subject><subject>Carbon - metabolism</subject><subject>DNA Primers</subject><subject>Electrophoresis - methods</subject><subject>Molecular Sequence Data</subject><subject>PCR-DGGE</subject><subject>Phosphates - metabolism</subject><subject>Polymerase Chain Reaction - methods</subject><subject>rDNA</subject><subject>生物磷迁移</subject><subject>移动反应</subject><issn>1001-0742</issn><issn>1878-7320</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM1u1TAQhSMEoqXwCshCiF0udn4ch11V8SdVYgPraGKPE18SO_U4VPd5eFF81Vas5iw-HZ35iuK94Ie6rsTH48ER-U-Cc1HyrqkOFeftgTcHLrpnxaVQnSq7uuLPc35iLopXREfOedPy9mVxIaRoVav6y-LvtYflRI5YsGybA20zJCxB633dF0jOTyzECbyjlZjel-T-ZMCw3RuMzDhrMaJPTEMcg2cU9qiR2L1LM9vCcloxAiHTMzjPIoJOLvjSoIe0x3P7FMG4c8OEC8MFdYohD4l4HgVEcHpdvLCwEL55vFfFry-ff958K29_fP1-c31b6kp2qVSSK6VqGI2y2iJ0nbDQSmgrbmSlbdUqYcYKx643YLQFa3Wj674TPSopdX1VfHjovQdvwU_DMT-T9dBwRBrw7Jk32fJ_cIvhbkdKw-pI47KAx7DTILPbmvcqg28fwX1c0QxbdCvE0_DkPwPvHgA9Bz_dZR_DCPq3dQsOGZGy7nn9D3Y3nGs</recordid><startdate>2005</startdate><enddate>2005</enddate><creator>Yu, Shui-Li</creator><creator>Liu, Ya-Nan</creator><creator>Jing, Guo-Lin</creator><creator>Zhao, Bing-Jie</creator><creator>Guo, Si-Yuan</creator><general>School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>WU4</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>2005</creationdate><title>Analysis of phosphate-accumulating organisms cultivated under different carbon sources with polymerase chain reaction-denaturing gradient gel electrophoresis assay</title><author>Yu, Shui-Li ; 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They were supplied with sewage, glucose and sodium acetate as carbon sources respectively and showed high phosphorus removal performance. The results of denaturing gradient gel electrophoresis(DGGE) of polymerase chain reaction-amplified (PCR) 16S rDNA fragments demonstrated that β-protebacteria, Actinomyces sp. and y-protebactefia only exited in 1# reactor. The microbiological diversity of 1# reactor exceeded the other two reactors. Flavobacterium, Bacillales, Actinomyces, Actinobacteridae and uncultured bacteria(AF527584, AF502204, AY592749, AB076862, AJ619051, AF495454 and AY133070) could be detected in the biological phosphorus removal reactors.</abstract><cop>Netherlands</cop><pub>School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China</pub><pmid>16158589</pmid><doi>10.3321/j.issn:1001-0742.2005.04.017</doi><tpages>4</tpages></addata></record> |
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| ispartof | Journal of environmental sciences (China), 2005, Vol.17 (4), p.611-614 |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | 16S Bacteria - metabolism Base Sequence Carbon - metabolism DNA Primers Electrophoresis - methods Molecular Sequence Data PCR-DGGE Phosphates - metabolism Polymerase Chain Reaction - methods rDNA 生物磷迁移 移动反应 |
| title | Analysis of phosphate-accumulating organisms cultivated under different carbon sources with polymerase chain reaction-denaturing gradient gel electrophoresis assay |
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