Screening of Lectins in Crab and Shrimp from Fujian Coast of China
Twelve species of crustacean from Fujian coast were examined for lectins with different animal erythrocytes. Serum extracts fromall of 12 species showed agglutinating activity against at least two types of the erythrocytes used, which revealed the existence of lectins in these species. There were fi...
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| Veröffentlicht in: | Marine science bulletin 2005-03, Vol.7 (1), p.77-81 |
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| description | Twelve species of crustacean from Fujian coast were examined for lectins with different animal erythrocytes. Serum extracts fromall of 12 species showed agglutinating activity against at least two types of the erythrocytes used, which revealed the existence of lectins in these species. There were five species ( Penaeus japonicus, Lophosquilla costata, Charybdis feriatus, Portunus pelagicus, Scylla serrata ) whose serums could agglutinate all the erythrocytes tested. The lowest serum protein concentration required to produce erythrocytes agglutination varied remarkably, ranging from 0.7 μg/mL to 8 080 μg/mL. The strongest activity was shown in the agglutination of rabbit erythrocyte by serum of Penaeus vanaminas. Inhibition assays performed with seven mono- and bisaccharides showed that agglutination of quail erythrocytes by serums of three species (Portunus pelagicus, Scylla serrata and Sesarma sp. ) were not inhibited by any sugars, while others were inhibited by at least three types of sugars. The assay of temperature influence on agglutinating activity showed that only Penaeusjaponicus retained activity when the serum was heated to 90℃, and other serums lost their activity at 40 ℃, 50 ℃, 60 ℃, 70 ℃, 80 ℃ for 10 minutes, respectively. |
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Serum extracts fromall of 12 species showed agglutinating activity against at least two types of the erythrocytes used, which revealed the existence of lectins in these species. There were five species ( Penaeus japonicus, Lophosquilla costata, Charybdis feriatus, Portunus pelagicus, Scylla serrata ) whose serums could agglutinate all the erythrocytes tested. The lowest serum protein concentration required to produce erythrocytes agglutination varied remarkably, ranging from 0.7 μg/mL to 8 080 μg/mL. The strongest activity was shown in the agglutination of rabbit erythrocyte by serum of Penaeus vanaminas. Inhibition assays performed with seven mono- and bisaccharides showed that agglutination of quail erythrocytes by serums of three species (Portunus pelagicus, Scylla serrata and Sesarma sp. ) were not inhibited by any sugars, while others were inhibited by at least three types of sugars. The assay of temperature influence on agglutinating activity showed that only Penaeusjaponicus retained activity when the serum was heated to 90℃, and other serums lost their activity at 40 ℃, 50 ℃, 60 ℃, 70 ℃, 80 ℃ for 10 minutes, respectively.</description><identifier>ISSN: 1000-9620</identifier><language>eng</language><publisher>College of Oceanography and Environmental Science, Xiamen University, Xiamen 361005, Fujian ,China</publisher><subject>Marine ; 外源凝集素 ; 福建海岸 ; 红血球胶合 ; 血凝素</subject><ispartof>Marine science bulletin, 2005-03, Vol.7 (1), p.77-81</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/85383X/85383X.jpg</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>戴聪杰</creatorcontrib><title>Screening of Lectins in Crab and Shrimp from Fujian Coast of China</title><title>Marine science bulletin</title><addtitle>Marina Science Bulletin</addtitle><description>Twelve species of crustacean from Fujian coast were examined for lectins with different animal erythrocytes. Serum extracts fromall of 12 species showed agglutinating activity against at least two types of the erythrocytes used, which revealed the existence of lectins in these species. There were five species ( Penaeus japonicus, Lophosquilla costata, Charybdis feriatus, Portunus pelagicus, Scylla serrata ) whose serums could agglutinate all the erythrocytes tested. The lowest serum protein concentration required to produce erythrocytes agglutination varied remarkably, ranging from 0.7 μg/mL to 8 080 μg/mL. The strongest activity was shown in the agglutination of rabbit erythrocyte by serum of Penaeus vanaminas. Inhibition assays performed with seven mono- and bisaccharides showed that agglutination of quail erythrocytes by serums of three species (Portunus pelagicus, Scylla serrata and Sesarma sp. ) were not inhibited by any sugars, while others were inhibited by at least three types of sugars. The assay of temperature influence on agglutinating activity showed that only Penaeusjaponicus retained activity when the serum was heated to 90℃, and other serums lost their activity at 40 ℃, 50 ℃, 60 ℃, 70 ℃, 80 ℃ for 10 minutes, respectively.</description><subject>Marine</subject><subject>外源凝集素</subject><subject>福建海岸</subject><subject>红血球胶合</subject><subject>血凝素</subject><issn>1000-9620</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNotkMFOwzAQRHMAiVL4B4sDnCKtk8aJjxBRQIrEob1ba3eTOKR2GydC_D1G5bTS7NPszlwlKw4AqRQZ3CS3IQwABRclrJKXnZmInHUd8y1ryMzWBWYdqyfUDN2B7frJHk-snfyRbZfBYtx5DPMfX_fW4V1y3eIY6P5_rpP99nVfv6fN59tH_dykRoJMqwNqhKyUh1JyNFAIyIWUWhcV5hmVpio2EoiiwDWQllmJmAsthEStK5Ovk6eL7Te6Fl2nBr9MLh5U_c-sFWUxE8SYMpKPF_I0-fNCYVZHGwyNIzryS1C8FPGPgkfw4QKa3rvuHEtQGs1Xa0dSvJCbiotN_guTGl2-</recordid><startdate>20050301</startdate><enddate>20050301</enddate><creator>戴聪杰</creator><general>College of Oceanography and Environmental Science, Xiamen University, Xiamen 361005, Fujian ,China</general><general>Department of Biology, Quanzhou Normal College, Quanzhou 362000, Fujian, China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>WU4</scope><scope>~WA</scope><scope>7TN</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20050301</creationdate><title>Screening of Lectins in Crab and Shrimp from Fujian Coast of China</title><author>戴聪杰</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c909-8daba0279d791ac05603699bb58a32e7c85490eebb51b0eb927aa36b669abb8c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Marine</topic><topic>外源凝集素</topic><topic>福建海岸</topic><topic>红血球胶合</topic><topic>血凝素</topic><toplevel>online_resources</toplevel><creatorcontrib>戴聪杰</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库-自然科学-生物科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Oceanic Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Marine science bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>戴聪杰</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Screening of Lectins in Crab and Shrimp from Fujian Coast of China</atitle><jtitle>Marine science bulletin</jtitle><addtitle>Marina Science Bulletin</addtitle><date>2005-03-01</date><risdate>2005</risdate><volume>7</volume><issue>1</issue><spage>77</spage><epage>81</epage><pages>77-81</pages><issn>1000-9620</issn><abstract>Twelve species of crustacean from Fujian coast were examined for lectins with different animal erythrocytes. Serum extracts fromall of 12 species showed agglutinating activity against at least two types of the erythrocytes used, which revealed the existence of lectins in these species. There were five species ( Penaeus japonicus, Lophosquilla costata, Charybdis feriatus, Portunus pelagicus, Scylla serrata ) whose serums could agglutinate all the erythrocytes tested. The lowest serum protein concentration required to produce erythrocytes agglutination varied remarkably, ranging from 0.7 μg/mL to 8 080 μg/mL. The strongest activity was shown in the agglutination of rabbit erythrocyte by serum of Penaeus vanaminas. Inhibition assays performed with seven mono- and bisaccharides showed that agglutination of quail erythrocytes by serums of three species (Portunus pelagicus, Scylla serrata and Sesarma sp. ) were not inhibited by any sugars, while others were inhibited by at least three types of sugars. The assay of temperature influence on agglutinating activity showed that only Penaeusjaponicus retained activity when the serum was heated to 90℃, and other serums lost their activity at 40 ℃, 50 ℃, 60 ℃, 70 ℃, 80 ℃ for 10 minutes, respectively.</abstract><pub>College of Oceanography and Environmental Science, Xiamen University, Xiamen 361005, Fujian ,China</pub><tpages>5</tpages></addata></record> |
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| ispartof | Marine science bulletin, 2005-03, Vol.7 (1), p.77-81 |
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| language | eng |
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| source | Alma/SFX Local Collection |
| subjects | Marine 外源凝集素 福建海岸 红血球胶合 血凝素 |
| title | Screening of Lectins in Crab and Shrimp from Fujian Coast of China |
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