降钙素基因相关肽对脂多糖诱导的巨噬细胞表达髓样细胞触发受体-1的影响

目的：探讨降钙素基因相关肽（CGRP）对脂多糖（LPS）诱导的小鼠巨噬细胞表达髓样细胞触发受体-1（TREM-1）的影响及其信号转导途径。方法：采用反转录-聚合酶链式反应（RT-PCR）观察巨噬细胞TREM-1mRNA表达量的变化,应用流式细胞术检测巨噬细胞膜表面TREM-1蛋白的表达。结果：CGRP对未受刺激的巨噬细胞表达TREM-1无明显影响,LPS可诱导巨噬细胞表达TREM-1;CGRP预处理呈剂量和时间依赖性上调LPS所致的巨噬细胞TREM-1mRNA的表达;同时,CGRP上调LPS所致的巨噬细胞膜表面TREM-1蛋白的表达;上述作用均可被PKC阻断剂H-7和PKA阻断剂H-89部分逆...

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Veröffentlicht in:	Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban 2010, Vol.35 (2), p.100-106
1. Verfasser:	李云超管茶香周漾孙国瑛施萌邬晶李文杰
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Schlagworte:	巨噬细胞脂多糖降钙素基因相关肽髓样细胞触发受体-1
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container_title	Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban
container_volume	35
creator	李云超管茶香周漾孙国瑛施萌邬晶李文杰
description	目的：探讨降钙素基因相关肽（CGRP）对脂多糖（LPS）诱导的小鼠巨噬细胞表达髓样细胞触发受体-1（TREM-1）的影响及其信号转导途径。方法：采用反转录-聚合酶链式反应（RT-PCR）观察巨噬细胞TREM-1mRNA表达量的变化,应用流式细胞术检测巨噬细胞膜表面TREM-1蛋白的表达。结果：CGRP对未受刺激的巨噬细胞表达TREM-1无明显影响,LPS可诱导巨噬细胞表达TREM-1;CGRP预处理呈剂量和时间依赖性上调LPS所致的巨噬细胞TREM-1mRNA的表达;同时,CGRP上调LPS所致的巨噬细胞膜表面TREM-1蛋白的表达;上述作用均可被PKC阻断剂H-7和PKA阻断剂H-89部分逆转（P〈0.05）。结论：CGRP上调LPS诱导的巨噬细胞表达TREM-1,其胞内信号转导途径与PKA和PKC有关。
doi_str_mv	10.3969/j.issn.1672-7347.2010.02.002
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Yi xue ban</title><addtitle>Journal of Central South University （Medical Sciences） Journal of Central South University （Medical Sciences）</addtitle><description>目的：探讨降钙素基因相关肽（CGRP）对脂多糖（LPS）诱导的小鼠巨噬细胞表达髓样细胞触发受体-1（TREM-1）的影响及其信号转导途径。方法：采用反转录-聚合酶链式反应（RT-PCR）观察巨噬细胞TREM-1mRNA表达量的变化,应用流式细胞术检测巨噬细胞膜表面TREM-1蛋白的表达。结果：CGRP对未受刺激的巨噬细胞表达TREM-1无明显影响,LPS可诱导巨噬细胞表达TREM-1;CGRP预处理呈剂量和时间依赖性上调LPS所致的巨噬细胞TREM-1mRNA的表达;同时,CGRP上调LPS所致的巨噬细胞膜表面TREM-1蛋白的表达;上述作用均可被PKC阻断剂H-7和PKA阻断剂H-89部分逆转（P〈0.05）。结论：CGRP上调LPS诱导的巨噬细胞表达TREM-1,其胞内信号转导途径与PKA和PKC有关。</description><subject>巨噬细胞</subject><subject>脂多糖</subject><subject>降钙素基因相关肽</subject><subject>髓样细胞触发受体-1</subject><issn>1672-7347</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNo9kEtLAlEcxe-iIDG_RAStZrqvedxlSC8Q2riXuTN3dFRGaohqa9ZOpIRChTKIEgpEDOyln6bbjN-iESU4cOD8f_wPHADWEVQJ09lmUfWCwFeRbmDFINRQMYxPEKsQ4iWQ-M9XQCoIPA4hY4zoppEAfNqqT69b4VtX3n_KTjfsvMuLYVQdy_5HVKvKx3Y4vIn6A9n_Dts1OerJ1mv4dRmd30UPvWgymb40f7ujRfL8JBtXsnH7M24qaIaPB7JZXwXLrlUORGrhSZDd2c6m95TMwe5-eiuj2BrDCuNUFwIJQoWDTAtjzeTYpprGDcxNTF2CMOXcFHrsELqC6pBwbDq2QzCzNZIEG_O3J5bvWn4-V6wcH_lxYa7gn5Wc09kkEMeKybU5aRcqfv7Qi1lu2SXXK4scIYgZEFPyByMNhw4</recordid><startdate>2010</startdate><enddate>2010</enddate><creator>李云超管茶香周漾孙国瑛施萌邬晶李文杰</creator><general>中南大学湘雅医学院临床医学系,长沙,410078%中南大学湘雅医学院生理学系,长沙,410078</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>2010</creationdate><title>降钙素基因相关肽对脂多糖诱导的巨噬细胞表达髓样细胞触发受体-1的影响</title><author>李云超管茶香周漾孙国瑛施萌邬晶李文杰</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c592-9b46ee1e34ed18a2258b2c455b72b824f3124bb8e612400fe4603b28dcd329c53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2010</creationdate><topic>巨噬细胞</topic><topic>脂多糖</topic><topic>降钙素基因相关肽</topic><topic>髓样细胞触发受体-1</topic><toplevel>online_resources</toplevel><creatorcontrib>李云超管茶香周漾孙国瑛施萌邬晶李文杰</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>李云超管茶香周漾孙国瑛施萌邬晶李文杰</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>降钙素基因相关肽对脂多糖诱导的巨噬细胞表达髓样细胞触发受体-1的影响</atitle><jtitle>Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban</jtitle><addtitle>Journal of Central South University （Medical Sciences） Journal of Central South University （Medical Sciences）</addtitle><date>2010</date><risdate>2010</risdate><volume>35</volume><issue>2</issue><spage>100</spage><epage>106</epage><pages>100-106</pages><issn>1672-7347</issn><abstract>目的：探讨降钙素基因相关肽（CGRP）对脂多糖（LPS）诱导的小鼠巨噬细胞表达髓样细胞触发受体-1（TREM-1）的影响及其信号转导途径。方法：采用反转录-聚合酶链式反应（RT-PCR）观察巨噬细胞TREM-1mRNA表达量的变化,应用流式细胞术检测巨噬细胞膜表面TREM-1蛋白的表达。结果：CGRP对未受刺激的巨噬细胞表达TREM-1无明显影响,LPS可诱导巨噬细胞表达TREM-1;CGRP预处理呈剂量和时间依赖性上调LPS所致的巨噬细胞TREM-1mRNA的表达;同时,CGRP上调LPS所致的巨噬细胞膜表面TREM-1蛋白的表达;上述作用均可被PKC阻断剂H-7和PKA阻断剂H-89部分逆转（P〈0.05）。结论：CGRP上调LPS诱导的巨噬细胞表达TREM-1,其胞内信号转导途径与PKA和PKC有关。</abstract><pub>中南大学湘雅医学院临床医学系,长沙,410078%中南大学湘雅医学院生理学系,长沙,410078</pub><doi>10.3969/j.issn.1672-7347.2010.02.002</doi><tpages>7</tpages></addata></record>
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subjects	巨噬细胞脂多糖降钙素基因相关肽髓样细胞触发受体-1
title	降钙素基因相关肽对脂多糖诱导的巨噬细胞表达髓样细胞触发受体-1的影响
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