HSP70抑制H2O2所致C2C12肌原细胞Smac从线粒体的释放及细胞凋亡
目的：阐明HSP70对H2O2，所致C2C12肌原细胞凋亡的影响及其作用机制。方法：采用基因瞬间转染技术使细胞HSP0或／和Smac高表达，采用Hoechst 33258染色观察H2O2(0．5mmol／L)处理转基因C2C12肌原细胞不同时间后细胞核形态学改变并计算凋亡核百分率。DNA抽提及琼脂糖电泳观察凋亡特征性梯带。采用caspase活性定量检测试剂盒及蛋白质印迹分析caspase-9和caspase-3的活化。利用免疫荧光分析HSP70对H2O2，所致Smac从线粒体释放的影响。结果：HSP70对H2O2及Smac所致C2C12肌原细胞凋亡具有明显的抑制作用，凋亡核百分率明显降低，DN...
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| Veröffentlicht in: | Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban 2005, Vol.30 (1), p.32-37 |
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| container_title | Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban |
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| creator | 蒋碧梅 肖卫民 石永忠 刘梅冬 肖献忠 |
| description | 目的：阐明HSP70对H2O2，所致C2C12肌原细胞凋亡的影响及其作用机制。方法：采用基因瞬间转染技术使细胞HSP0或／和Smac高表达，采用Hoechst 33258染色观察H2O2(0．5mmol／L)处理转基因C2C12肌原细胞不同时间后细胞核形态学改变并计算凋亡核百分率。DNA抽提及琼脂糖电泳观察凋亡特征性梯带。采用caspase活性定量检测试剂盒及蛋白质印迹分析caspase-9和caspase-3的活化。利用免疫荧光分析HSP70对H2O2，所致Smac从线粒体释放的影响。结果：HSP70对H2O2及Smac所致C2C12肌原细胞凋亡具有明显的抑制作用，凋亡核百分率明显降低，DNA电泳未出现明显“梯状”条带；caspase-9和caspase-3的活化明显受到抑制；并进一步发现HSP70可抑制H2O2所致C2C12肌原细胞Smac从线粒体释放。结论：HSP70可通过抑制Smac从线粒体释放来抑制H2O2，所致C2C12肌原细胞凋亡。 |
| doi_str_mv | 10.3321/j.issn:1672-7347.2005.01.007 |
| format | Article |
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Journal of Central South University. Yi xue ban, 2005, Vol.30 (1), p.32-37</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/90655A/90655A.jpg</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids></links><search><creatorcontrib>蒋碧梅 肖卫民 石永忠 刘梅冬 肖献忠</creatorcontrib><title>HSP70抑制H2O2所致C2C12肌原细胞Smac从线粒体的释放及细胞凋亡</title><title>Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban</title><addtitle>Journal of Central South University (Medical Sciences) Journal of Central South University (Medical Sciences)</addtitle><description>目的：阐明HSP70对H2O2，所致C2C12肌原细胞凋亡的影响及其作用机制。方法：采用基因瞬间转染技术使细胞HSP0或／和Smac高表达，采用Hoechst 33258染色观察H2O2(0．5mmol／L)处理转基因C2C12肌原细胞不同时间后细胞核形态学改变并计算凋亡核百分率。DNA抽提及琼脂糖电泳观察凋亡特征性梯带。采用caspase活性定量检测试剂盒及蛋白质印迹分析caspase-9和caspase-3的活化。利用免疫荧光分析HSP70对H2O2，所致Smac从线粒体释放的影响。结果：HSP70对H2O2及Smac所致C2C12肌原细胞凋亡具有明显的抑制作用，凋亡核百分率明显降低，DNA电泳未出现明显“梯状”条带；caspase-9和caspase-3的活化明显受到抑制；并进一步发现HSP70可抑制H2O2所致C2C12肌原细胞Smac从线粒体释放。结论：HSP70可通过抑制Smac从线粒体释放来抑制H2O2，所致C2C12肌原细胞凋亡。</description><subject>C2C12肌原细胞</subject><subject>H2O2</subject><subject>HSP70</subject><subject>Smac</subject><subject>细胞凋亡</subject><issn>1672-7347</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNo9z81Kw0AUBeBZKFhqX0IEV4kzdzoziTsJaoVihbovk0mmTVtTNIi6EytGq1T8WblQfAURFy3qyzRNfQsjFVcHDh_3chBaJNikFMhy0wyiKFwhXIAhaFGYgDEzMTExFjMo99_PoUIUBS7Gtm1Tbokc2ipVtwUe926Ti_cSVGB8eTKJ3xxwCExOr5P-czo8n3SfqrtSjYb9dPCVvt6NPu7Tx7PvuDd--ExuelORxFejwcs8mtWyHfmFv8yj6vrajlMyypWNTWe1bChmC4NpqigRwrO4SxXXPteeLXTR10xynwN42hJEA5MuUcoD5meSA5bAqLYwzaOl6dVDGWoZ1mvNzsF-mP2rNcLjlnf0ux6TbHsmF6ZSNTphfS_IrCtVSwdtv0YYMGYTQX8A61NxPg</recordid><startdate>2005</startdate><enddate>2005</enddate><creator>蒋碧梅 肖卫民 石永忠 刘梅冬 肖献忠</creator><general>中南大学湘雅医学院病理生理学教研室,长沙,410078</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>2005</creationdate><title>HSP70抑制H2O2所致C2C12肌原细胞Smac从线粒体的释放及细胞凋亡</title><author>蒋碧梅 肖卫民 石永忠 刘梅冬 肖献忠</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c597-5f3c3177d86b3c6fe6fd97f4ef5a6e622df871f25ab1ccd25ed86620a253f803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2005</creationdate><topic>C2C12肌原细胞</topic><topic>H2O2</topic><topic>HSP70</topic><topic>Smac</topic><topic>细胞凋亡</topic><toplevel>online_resources</toplevel><creatorcontrib>蒋碧梅 肖卫民 石永忠 刘梅冬 肖献忠</creatorcontrib><collection>维普_期刊</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>维普中文期刊数据库</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Zhong nan da xue xue bao. 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Yi xue ban</jtitle><addtitle>Journal of Central South University (Medical Sciences) Journal of Central South University (Medical Sciences)</addtitle><date>2005</date><risdate>2005</risdate><volume>30</volume><issue>1</issue><spage>32</spage><epage>37</epage><pages>32-37</pages><issn>1672-7347</issn><abstract>目的：阐明HSP70对H2O2，所致C2C12肌原细胞凋亡的影响及其作用机制。方法：采用基因瞬间转染技术使细胞HSP0或／和Smac高表达，采用Hoechst 33258染色观察H2O2(0．5mmol／L)处理转基因C2C12肌原细胞不同时间后细胞核形态学改变并计算凋亡核百分率。DNA抽提及琼脂糖电泳观察凋亡特征性梯带。采用caspase活性定量检测试剂盒及蛋白质印迹分析caspase-9和caspase-3的活化。利用免疫荧光分析HSP70对H2O2，所致Smac从线粒体释放的影响。结果：HSP70对H2O2及Smac所致C2C12肌原细胞凋亡具有明显的抑制作用，凋亡核百分率明显降低，DNA电泳未出现明显“梯状”条带；caspase-9和caspase-3的活化明显受到抑制；并进一步发现HSP70可抑制H2O2所致C2C12肌原细胞Smac从线粒体释放。结论：HSP70可通过抑制Smac从线粒体释放来抑制H2O2，所致C2C12肌原细胞凋亡。</abstract><pub>中南大学湘雅医学院病理生理学教研室,长沙,410078</pub><doi>10.3321/j.issn:1672-7347.2005.01.007</doi><tpages>6</tpages></addata></record> |
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| ispartof | Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban, 2005, Vol.30 (1), p.32-37 |
| issn | 1672-7347 |
| language | chi |
| recordid | cdi_wanfang_journals_hnykdx200501007 |
| source | Alma/SFX Local Collection |
| subjects | C2C12肌原细胞 H2O2 HSP70 Smac 细胞凋亡 |
| title | HSP70抑制H2O2所致C2C12肌原细胞Smac从线粒体的释放及细胞凋亡 |
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