Microarray screening of differentially expressed genes in DCX transfected U87 cells before and after irradiation

Radiation therapy plays a critical role in the treatment of neurogliocytoma and it is known that doublecortin (DCX)-transfected U87 cells can inhibit tumor cell growth. Microarray analysis to screen for differentially expressed genes in DCX-transfected U87 cells before and after radiation uncovered...

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Veröffentlicht in:核技术(英文版) 2012-04, Vol.23 (2), p.97-102
1. Verfasser: FENG Shuang WANG Jie JIANG Xin XU Yuanyuan ZHANG Haowen Cao Jianping YU Jiahua LIU Fenju
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description Radiation therapy plays a critical role in the treatment of neurogliocytoma and it is known that doublecortin (DCX)-transfected U87 cells can inhibit tumor cell growth. Microarray analysis to screen for differentially expressed genes in DCX-transfected U87 cells before and after radiation uncovered DCX-related genes, the functions of DCX, and downstream genes in radiation therapy of neurogliocytoma. Stably transfected U87 cells were constructed (DCX-U87) and the differentially expressed genes were screened by microarray analysis to compare U87 cells with DCX-U87 cells in both non-irradiated and irradiated conditions. Cells were irradiated using 6~Co 7-ray at a dose rate of 1.0 Gy/min. Mean values were subject to paired comparison analysis and genes with a p-value of less than 0.05 were analyzed. Differentially expressed genes can correlate with radiation sensitivity and DCX transfection. DCX and SPN proteins in DCX-U87 cells were detected by two groups of 0 and 10 Gy, but not the U87 cells, and their expression levels were higher in the 10 Gy group than in the 0 Gy group. The differential gene expression in DCX-U87 cells before and after radiation is helpful for future investigations into the mechanisms of radiation therapy in neurogliocytoma cells.
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The differential gene expression in DCX-U87 cells before and after radiation is helpful for future investigations into the mechanisms of radiation therapy in neurogliocytoma cells.</abstract><pub>School of Radiation Medicine and Protection, Jiangsu Key Laboratory of Radiation Medicine and Protection,Medical College of Soochow University, Suzhou 215123, China</pub><tpages>6</tpages></addata></record>
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subjects X架构
基因差异表达
基因芯片技术
射线照射
筛选
肿瘤细胞
转染
title Microarray screening of differentially expressed genes in DCX transfected U87 cells before and after irradiation
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