泰乐菌素高产菌株诱变选育
TQ464; 本研究采用化学诱变剂(硫酸二乙酯)和物理诱变(微波)相结合的方法,选育泰乐菌素高产菌株.以弗氏链霉菌(Streptomyces fradiae)BL-16菌株为出发菌株,发酵效价6526μg/ml,将出发菌株采用微波诱变,通过菌株初筛、摇瓶复筛获得产素能力较高的菌株BLK-5,其摇瓶效价为9525μg/ml,再经硫酸二乙酯诱变,初筛、摇瓶复筛获得产素能力更高的菌株10个高产突变菌株,并对其进行遗传稳定性试验,结果表明LKY-8生产代谢旺盛,遗传稳定性好,发酵效价11126μg/ml,比出发菌株提高了70.49％....
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| Veröffentlicht in: | 当代化工研究 2019 (22), p.111-112 |
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| creator | 刘鹏 徐雪风 陆建中 王维兴 |
| description | TQ464; 本研究采用化学诱变剂(硫酸二乙酯)和物理诱变(微波)相结合的方法,选育泰乐菌素高产菌株.以弗氏链霉菌(Streptomyces fradiae)BL-16菌株为出发菌株,发酵效价6526μg/ml,将出发菌株采用微波诱变,通过菌株初筛、摇瓶复筛获得产素能力较高的菌株BLK-5,其摇瓶效价为9525μg/ml,再经硫酸二乙酯诱变,初筛、摇瓶复筛获得产素能力更高的菌株10个高产突变菌株,并对其进行遗传稳定性试验,结果表明LKY-8生产代谢旺盛,遗传稳定性好,发酵效价11126μg/ml,比出发菌株提高了70.49％. |
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| title | 泰乐菌素高产菌株诱变选育 |
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