Development of real-time PCR method for rapid detection and quantification of Heterosigma akashiwo

Development of real-time PCR method for rapid detection and quantification of Heterosigma akashiwo

To rapidly detect the harmful algae H. akashiwo qualitatively and quantitatively, sequences of the 18S rDNA deduced from H. akashiwo were used for designing species-specific primers, and a RFQ-PCR （Realtime Fluorescent Quantitative Polymerase Chain Reaction） method was developed for quantitative det...

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Veröffentlicht in:Journal of Harbin Institute of Technology 2008-02, Vol.15 (1), p.118-123
1. Verfasser: 何闪英 于志刚 米铁柱
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分子探针
时间相关分析
硅藻门
荧光定量PCR
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creator 何闪英 于志刚 米铁柱
description To rapidly detect the harmful algae H. akashiwo qualitatively and quantitatively, sequences of the 18S rDNA deduced from H. akashiwo were used for designing species-specific primers, and a RFQ-PCR （Realtime Fluorescent Quantitative Polymerase Chain Reaction） method was developed for quantitative detection of H. akashiwo. Primer H. akashiwo and TaqMan probe were designed, and the specificity of primer was checked with PCR. A calibration curve was constructed with cycle threshold value against visual counted cell number. And the value of the curve was tested with other H. akashiwo samples, which were assayed with both the RFQ- PCR method and visual count under microscope.
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subjects 分子探针
时间相关分析
硅藻门
荧光定量PCR
title Development of real-time PCR method for rapid detection and quantification of Heterosigma akashiwo
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