弗氏柠檬酸菌甘油脱水酶基因在大肠杆菌中的克隆和表达

以弗氏柠檬酸菌（Citrobacter freundii）基因组DNA为模板，通过PCR得到甘油脱水酶（glycerol dehydratase）基因dhaB、dhaC、dhaE，克隆到表达载体pSE380上，得到重组质粒pSn-dhaBCE。将此重组质粒转化到E．coli JM109中，重组菌株SDS-PAGE结果显示有明显的61kD、22kD、16kD三条特异性蛋白条带出现。重组菌株经诱导表达，酶活力为11．59U／mL。...

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Veröffentlicht in:	Gongye weishengwu 2005, Vol.35 (3), p.10-13
1. Verfasser:	齐向辉朱绮霞韦宇拓黄鲲黄日波
Format:	Artikel
Sprache:	chi
Schlagworte:	SDS-PAGE 克隆克隆和表达基因组DNA 大肠杆菌弗氏柠檬酸菌柠檬酸甘油脱水酶表达酶基因
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container_title	Gongye weishengwu
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creator	齐向辉朱绮霞韦宇拓黄鲲黄日波
description	以弗氏柠檬酸菌（Citrobacter freundii）基因组DNA为模板，通过PCR得到甘油脱水酶（glycerol dehydratase）基因dhaB、dhaC、dhaE，克隆到表达载体pSE380上，得到重组质粒pSn-dhaBCE。将此重组质粒转化到E．coli JM109中，重组菌株SDS-PAGE结果显示有明显的61kD、22kD、16kD三条特异性蛋白条带出现。重组菌株经诱导表达，酶活力为11．59U／mL。
doi_str_mv	10.3969/j.issn.1001-6678.2005.03.003
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subjects	SDS-PAGE 克隆克隆和表达基因组DNA 大肠杆菌弗氏柠檬酸菌柠檬酸甘油脱水酶表达酶基因
title	弗氏柠檬酸菌甘油脱水酶基因在大肠杆菌中的克隆和表达
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