LncRNA MIR22HG调节miR-132-3p/TLR2轴对急性心肌梗死小鼠心肌细胞凋亡和心室重构的影响
R542.2; 目的:探讨长链非编码RNA miR-22宿主基因(lncRNA MIR22HG)调节miR-132-3p/Toll样受体2(TLR2)轴对急性心肌梗死(AMI)小鼠心肌细胞凋亡和心室重构的影响.方法:取C57BL/6J小鼠随机分为sham组、AMI组、AMI+sh-NC组、AMI+sh-MIR22HG组、AMI+sh-MIR22HG+antagomir-NC组、AMI+sh-MIR22HG+antagomir-132-3p组,每组10只,除去sham组外,其它组都进行冠状动脉左前降支结扎,sham组只开胸不结扎冠状动脉,其中AMI+sh-NC组、AMI+sh-MIR22HG组、...
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| Veröffentlicht in: | 广西医科大学学报 2023, Vol.40 (7), p.1123-1131 |
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| creator | 李延民 冯艳 魏燕云 王献忠 |
| description | R542.2; 目的:探讨长链非编码RNA miR-22宿主基因(lncRNA MIR22HG)调节miR-132-3p/Toll样受体2(TLR2)轴对急性心肌梗死(AMI)小鼠心肌细胞凋亡和心室重构的影响.方法:取C57BL/6J小鼠随机分为sham组、AMI组、AMI+sh-NC组、AMI+sh-MIR22HG组、AMI+sh-MIR22HG+antagomir-NC组、AMI+sh-MIR22HG+antagomir-132-3p组,每组10只,除去sham组外,其它组都进行冠状动脉左前降支结扎,sham组只开胸不结扎冠状动脉,其中AMI+sh-NC组、AMI+sh-MIR22HG组、AMI+sh-MIR22HG+antagomir-NC组、AMI+sh-MIR22HG+antagomir-132-3p组小鼠分别相对应的对尾静脉注射sh-NC、sh-MIR22HG、sh-MIR22HG+antagomir-NC、sh-MIR22HG+antagomir-132-3p.超声心动图评估小鼠心室重构和心脏功能;HE染色和Masson染色观察心肌组织病理学变化及纤维化;RT-qPCR检测心肌组织MIR22HG、miR-132-3p表达;TUNEL法检测心肌细胞凋亡;Western blotting检测心肌组织B细胞淋巴瘤-2(Bcl-2)、激活型半胱氨酸蛋白酶-3(cleved caspase-3)/半胱氨酸蛋白酶-3(cas-pase-3)、Ⅰ型胶原蛋白(collagen Ⅰ)、collagen Ⅲ和α-平滑肌肌动蛋白(α-SMA)表达;双荧光素酶报告基因实验分别验证MIR22HG和miR-132-3p、miR-132-3p和TLR2的关系.结果:与sham组比较,AMI组小鼠心功能显著降低,心肌组织损伤、心肌纤维化加重,心肌细胞凋亡率、MIR22HG、TLR2、cleved caspase-3/caspase-3、collagen Ⅰ、collagen Ⅲ和α-SMA蛋白表达水平显著升高,miR-132-3p和Bcl-2蛋白表达降低(P |
| doi_str_mv | 10.16190/j.cnki.45-1211/r.2023.07.007 |
| format | Article |
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Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/gxykdx/gxykdx.jpg</thumbnail><link.rule.ids>314,776,780,860,4010,27900,27901,27902</link.rule.ids></links><search><creatorcontrib>李延民</creatorcontrib><creatorcontrib>冯艳</creatorcontrib><creatorcontrib>魏燕云</creatorcontrib><creatorcontrib>王献忠</creatorcontrib><title>LncRNA MIR22HG调节miR-132-3p/TLR2轴对急性心肌梗死小鼠心肌细胞凋亡和心室重构的影响</title><title>广西医科大学学报</title><description>R542.2; 目的:探讨长链非编码RNA miR-22宿主基因(lncRNA MIR22HG)调节miR-132-3p/Toll样受体2(TLR2)轴对急性心肌梗死(AMI)小鼠心肌细胞凋亡和心室重构的影响.方法:取C57BL/6J小鼠随机分为sham组、AMI组、AMI+sh-NC组、AMI+sh-MIR22HG组、AMI+sh-MIR22HG+antagomir-NC组、AMI+sh-MIR22HG+antagomir-132-3p组,每组10只,除去sham组外,其它组都进行冠状动脉左前降支结扎,sham组只开胸不结扎冠状动脉,其中AMI+sh-NC组、AMI+sh-MIR22HG组、AMI+sh-MIR22HG+antagomir-NC组、AMI+sh-MIR22HG+antagomir-132-3p组小鼠分别相对应的对尾静脉注射sh-NC、sh-MIR22HG、sh-MIR22HG+antagomir-NC、sh-MIR22HG+antagomir-132-3p.超声心动图评估小鼠心室重构和心脏功能;HE染色和Masson染色观察心肌组织病理学变化及纤维化;RT-qPCR检测心肌组织MIR22HG、miR-132-3p表达;TUNEL法检测心肌细胞凋亡;Western blotting检测心肌组织B细胞淋巴瘤-2(Bcl-2)、激活型半胱氨酸蛋白酶-3(cleved caspase-3)/半胱氨酸蛋白酶-3(cas-pase-3)、Ⅰ型胶原蛋白(collagen Ⅰ)、collagen Ⅲ和α-平滑肌肌动蛋白(α-SMA)表达;双荧光素酶报告基因实验分别验证MIR22HG和miR-132-3p、miR-132-3p和TLR2的关系.结果:与sham组比较,AMI组小鼠心功能显著降低,心肌组织损伤、心肌纤维化加重,心肌细胞凋亡率、MIR22HG、TLR2、cleved caspase-3/caspase-3、collagen Ⅰ、collagen Ⅲ和α-SMA蛋白表达水平显著升高,miR-132-3p和Bcl-2蛋白表达降低(P<0.05);与AMI+sh-NC组比较,AMI+sh-MIR22HG组小鼠心功能改善,心肌组织损伤、心肌纤维化减轻,心肌细胞凋亡率、MIR22HG、TLR2、cleved caspase-3/caspase-3、collagen Ⅰ、collagen Ⅲ和α-SMA蛋白表达水</description><issn>1005-930X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNpjYFA1NNAzNDO0NNDP0kvOy87UMzHVNTQyNNQv0jMyMDLWMzDXMzAwZ2HgNDQwMNW1NDaI4GDgLS7OTDIwMDIyMzEyNOJkyPbJSw7yc1Tw9QwyMvJwf7Gh-UVXU25mkK6hsZGucYF-iE-Q0Yu9W56u3_msYemzhuVP9ze_aOp5tmj6s7W7n27of7lnAUTk-e62F83znrZ3P9m18OmkHqDg03VLXrb3PpvX8nxWy9O9G59O7uVhYE1LzClO5YXS3Azqbq4hzh665Yl5aYl56fFZ-aVFeUCZ-PSKyuyUCpAnDMyBXjAmXiUADc9srw</recordid><startdate>2023</startdate><enddate>2023</enddate><creator>李延民</creator><creator>冯艳</creator><creator>魏燕云</creator><creator>王献忠</creator><general>河北省邯郸市第一医院心内二科,邯郸 056002%河北省邯郸市第一医院检验科,邯郸 056002%河北省邯郸市第一医院新生儿科,邯郸 056002</general><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>2023</creationdate><title>LncRNA MIR22HG调节miR-132-3p/TLR2轴对急性心肌梗死小鼠心肌细胞凋亡和心室重构的影响</title><author>李延民 ; 冯艳 ; 魏燕云 ; 王献忠</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-wanfang_journals_gxykdx2023070073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2023</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>李延民</creatorcontrib><creatorcontrib>冯艳</creatorcontrib><creatorcontrib>魏燕云</creatorcontrib><creatorcontrib>王献忠</creatorcontrib><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>广西医科大学学报</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>李延民</au><au>冯艳</au><au>魏燕云</au><au>王献忠</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>LncRNA MIR22HG调节miR-132-3p/TLR2轴对急性心肌梗死小鼠心肌细胞凋亡和心室重构的影响</atitle><jtitle>广西医科大学学报</jtitle><date>2023</date><risdate>2023</risdate><volume>40</volume><issue>7</issue><spage>1123</spage><epage>1131</epage><pages>1123-1131</pages><issn>1005-930X</issn><abstract>R542.2; 目的:探讨长链非编码RNA miR-22宿主基因(lncRNA MIR22HG)调节miR-132-3p/Toll样受体2(TLR2)轴对急性心肌梗死(AMI)小鼠心肌细胞凋亡和心室重构的影响.方法:取C57BL/6J小鼠随机分为sham组、AMI组、AMI+sh-NC组、AMI+sh-MIR22HG组、AMI+sh-MIR22HG+antagomir-NC组、AMI+sh-MIR22HG+antagomir-132-3p组,每组10只,除去sham组外,其它组都进行冠状动脉左前降支结扎,sham组只开胸不结扎冠状动脉,其中AMI+sh-NC组、AMI+sh-MIR22HG组、AMI+sh-MIR22HG+antagomir-NC组、AMI+sh-MIR22HG+antagomir-132-3p组小鼠分别相对应的对尾静脉注射sh-NC、sh-MIR22HG、sh-MIR22HG+antagomir-NC、sh-MIR22HG+antagomir-132-3p.超声心动图评估小鼠心室重构和心脏功能;HE染色和Masson染色观察心肌组织病理学变化及纤维化;RT-qPCR检测心肌组织MIR22HG、miR-132-3p表达;TUNEL法检测心肌细胞凋亡;Western blotting检测心肌组织B细胞淋巴瘤-2(Bcl-2)、激活型半胱氨酸蛋白酶-3(cleved caspase-3)/半胱氨酸蛋白酶-3(cas-pase-3)、Ⅰ型胶原蛋白(collagen Ⅰ)、collagen Ⅲ和α-平滑肌肌动蛋白(α-SMA)表达;双荧光素酶报告基因实验分别验证MIR22HG和miR-132-3p、miR-132-3p和TLR2的关系.结果:与sham组比较,AMI组小鼠心功能显著降低,心肌组织损伤、心肌纤维化加重,心肌细胞凋亡率、MIR22HG、TLR2、cleved caspase-3/caspase-3、collagen Ⅰ、collagen Ⅲ和α-SMA蛋白表达水平显著升高,miR-132-3p和Bcl-2蛋白表达降低(P<0.05);与AMI+sh-NC组比较,AMI+sh-MIR22HG组小鼠心功能改善,心肌组织损伤、心肌纤维化减轻,心肌细胞凋亡率、MIR22HG、TLR2、cleved caspase-3/caspase-3、collagen Ⅰ、collagen Ⅲ和α-SMA蛋白表达水</abstract><pub>河北省邯郸市第一医院心内二科,邯郸 056002%河北省邯郸市第一医院检验科,邯郸 056002%河北省邯郸市第一医院新生儿科,邯郸 056002</pub><doi>10.16190/j.cnki.45-1211/r.2023.07.007</doi></addata></record> |
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| title | LncRNA MIR22HG调节miR-132-3p/TLR2轴对急性心肌梗死小鼠心肌细胞凋亡和心室重构的影响 |
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