Lentivirus-mediated RNA Interference and Over-expression of CDK2AP1 CDNA Regulate CDK2AP1 Expression in Human Lung Cancer A549 Cells
Cyclin-dependent kinase 2-associated protein 1（CDK2AP1）, a cell growth inhibitory factor, is abnormally expressed in cancer cells, and might be implicated in the development of lung cancer. However, no studies on the function of CDK2AP1 in human lung cancer have been yet reported. In this study, ove...
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| Veröffentlicht in: | 高等学校化学研究（英文版） 2011-05, Vol.27 (3), p.445-449 |
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| container_title | 高等学校化学研究（英文版） |
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| creator | GAO Nan ZHANG Xing-yi JIANO Rui WANG Guan LI Jin-dong J1NCheng-yan SUN Mei |
| description | Cyclin-dependent kinase 2-associated protein 1（CDK2AP1）, a cell growth inhibitory factor, is abnormally expressed in cancer cells, and might be implicated in the development of lung cancer. However, no studies on the function of CDK2AP1 in human lung cancer have been yet reported. In this study, overexpressing lentiviral vectors containing full-length CDK2AP1 cDNA and CDK2AP1 shRNA（short hairpin RNA） were constructed. Our results show that infecting A549 cells with lentivirus containing CDK2AP1 shRNA or full-length CDK2AP1 cDNA results in significantly down- or up-regulated the expression of CDK2AP1, respectively, thereby providing reliable tools for studying the function of CDK2AP1 in pulmonary carcinogenesis. Our data of MTT assay and flowcytometric analysis demonstrate that CDK2AP1 plays an important role in the proliferation/growth and cell cycling of A549 cells in vitro, and further investigation into its underlying mechanism of pulmonary carcinogenesis is needed. |
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However, no studies on the function of CDK2AP1 in human lung cancer have been yet reported. In this study, overexpressing lentiviral vectors containing full-length CDK2AP1 cDNA and CDK2AP1 shRNA（short hairpin RNA） were constructed. Our results show that infecting A549 cells with lentivirus containing CDK2AP1 shRNA or full-length CDK2AP1 cDNA results in significantly down- or up-regulated the expression of CDK2AP1, respectively, thereby providing reliable tools for studying the function of CDK2AP1 in pulmonary carcinogenesis. Our data of MTT assay and flowcytometric analysis demonstrate that CDK2AP1 plays an important role in the proliferation/growth and cell cycling of A549 cells in vitro, and further investigation into its underlying mechanism of pulmonary carcinogenesis is needed.</description><identifier>ISSN: 1005-9040</identifier><identifier>EISSN: 2210-3171</identifier><language>eng</language><publisher>Department of Thoracic Surgery,the Second Hospital of Jilin University, Changchun 130041, R. P. China%Department of Orthopedic Surgery, China-Japan Union Hospital of Jilin University, Changchun 130033, P. R. China%Department of Cardiac Medicine,the Second Hospital of Jilin University, Changchun 130041, P. R. China%Department of Pathology,the Second Hospital of Jilin University, Changchun 130041, P. R. China</publisher><subject>A549细胞 ; RNA干扰 ; 人类 ; 全长cDNA ; 周期蛋白依赖性激酶 ; 慢病毒 ; 肺癌 ; 过度表达</subject><ispartof>高等学校化学研究（英文版）, 2011-05, Vol.27 (3), p.445-449</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/86071X/86071X.jpg</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>GAO Nan ZHANG Xing-yi JIANO Rui WANG Guan LI Jin-dong J1NCheng-yan SUN Mei</creatorcontrib><title>Lentivirus-mediated RNA Interference and Over-expression of CDK2AP1 CDNA Regulate CDK2AP1 Expression in Human Lung Cancer A549 Cells</title><title>高等学校化学研究（英文版）</title><addtitle>Chemical Research in Chinese University</addtitle><description>Cyclin-dependent kinase 2-associated protein 1（CDK2AP1）, a cell growth inhibitory factor, is abnormally expressed in cancer cells, and might be implicated in the development of lung cancer. 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Our data of MTT assay and flowcytometric analysis demonstrate that CDK2AP1 plays an important role in the proliferation/growth and cell cycling of A549 cells in vitro, and further investigation into its underlying mechanism of pulmonary carcinogenesis is needed.</description><subject>A549细胞</subject><subject>RNA干扰</subject><subject>人类</subject><subject>全长cDNA</subject><subject>周期蛋白依赖性激酶</subject><subject>慢病毒</subject><subject>肺癌</subject><subject>过度表达</subject><issn>1005-9040</issn><issn>2210-3171</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNpFkE1Lw0AYhIMoWKv_YT16CLz7lWSPIVZbDFaKnstm8yZNSTd109T07g93oaKngeGZGZiLYMIYhZDTmF4GEwogQwUCroObvt8CcBVFYhJ852gPzbFxQx_usGz0AUuyek3Jwh7QVejQGiTalmR5RBfiuHfY901nSVeR7PGFpW_Uqw-ssB5aH_9zZ_9sY8l82GlL8sHWJNO-05FUCkUybNv-NriqdNvj3a9Og4-n2Xs2D_Pl8yJL89AwKg9hIeIoNgUyaSKNDIEmUClQlYoLZpJS8rIUSqoiYZHRSQE0kpEwiRAxxtpQPg0ezr1f2lba1uttNzjrF9d1OY6b8bRlQClwYMyz92fWbDpbfzae3rtmp91pzRPg0n_JfwDLS2jp</recordid><startdate>20110501</startdate><enddate>20110501</enddate><creator>GAO Nan ZHANG Xing-yi JIANO Rui WANG Guan LI Jin-dong J1NCheng-yan SUN Mei</creator><general>Department of Thoracic Surgery,the Second Hospital of Jilin University, Changchun 130041, R. P. China%Department of Orthopedic Surgery, China-Japan Union Hospital of Jilin University, Changchun 130033, P. R. China%Department of Cardiac Medicine,the Second Hospital of Jilin University, Changchun 130041, P. R. China%Department of Pathology,the Second Hospital of Jilin University, Changchun 130041, P. R. 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However, no studies on the function of CDK2AP1 in human lung cancer have been yet reported. In this study, overexpressing lentiviral vectors containing full-length CDK2AP1 cDNA and CDK2AP1 shRNA（short hairpin RNA） were constructed. Our results show that infecting A549 cells with lentivirus containing CDK2AP1 shRNA or full-length CDK2AP1 cDNA results in significantly down- or up-regulated the expression of CDK2AP1, respectively, thereby providing reliable tools for studying the function of CDK2AP1 in pulmonary carcinogenesis. Our data of MTT assay and flowcytometric analysis demonstrate that CDK2AP1 plays an important role in the proliferation/growth and cell cycling of A549 cells in vitro, and further investigation into its underlying mechanism of pulmonary carcinogenesis is needed.</abstract><pub>Department of Thoracic Surgery,the Second Hospital of Jilin University, Changchun 130041, R. P. China%Department of Orthopedic Surgery, China-Japan Union Hospital of Jilin University, Changchun 130033, P. R. China%Department of Cardiac Medicine,the Second Hospital of Jilin University, Changchun 130041, P. R. China%Department of Pathology,the Second Hospital of Jilin University, Changchun 130041, P. R. China</pub><tpages>5</tpages></addata></record> |
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| ispartof | 高等学校化学研究（英文版）, 2011-05, Vol.27 (3), p.445-449 |
| issn | 1005-9040 2210-3171 |
| language | eng |
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| source | Alma/SFX Local Collection |
| subjects | A549细胞 RNA干扰 人类 全长cDNA 周期蛋白依赖性激酶 慢病毒 肺癌 过度表达 |
| title | Lentivirus-mediated RNA Interference and Over-expression of CDK2AP1 CDNA Regulate CDK2AP1 Expression in Human Lung Cancer A549 Cells |
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