Imprinted Human Serum Albumin with Antioxidant Activity

Imprinted Human Serum Albumin with Antioxidant Activity

In order to create a new mimic of glutathione peroxidase（GPx）, bioimprinting was used to generate gluta-thione（GSH） binding site and chemical modification was used to incorporate catalytic group selenocystine（Sec）. Human serum albumin（HSA） and S-substituted dinitrophenyl glutathione（GSH-S-DNP） were...

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Veröffentlicht in:Chemical research in Chinese universities 2011-03, Vol.27 (2), p.258-263
1. Verfasser: SHEN Na YAN Fei GUO Yi LU Shao-wu GONG Ping-sheng XU Ya-wei YAN Gang-lin MU Ying LUO Gui-min
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GSH
HSA
人血清白蛋白
化学改性
抗氧化活性
硒代胱氨酸
谷胱甘肽过氧化物酶
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description In order to create a new mimic of glutathione peroxidase（GPx）, bioimprinting was used to generate gluta-thione（GSH） binding site and chemical modification was used to incorporate catalytic group selenocystine（Sec）. Human serum albumin（HSA） and S-substituted dinitrophenyl glutathione（GSH-S-DNP） were chosen as the imprinted matrix and imprinting template, respectively, to generate a GSH-imprinted protein（GSH-HSA） by bioimprinting. Sec was incorporated into the GSH-HSA by chemical modification to give a new GPx mimic（Se-GSH-HSA）. Se-GSH-HSA displayed considerably higher GPx activity than non-printed HSA（Se-HSA）. The enzymic properties and kinetics of Se-GSH-HSA were studied. Moreover, Se-GSH-HSA was confirmed to have stronger antioxidant ability to protect mitochondria against oxidative damage with ferrous sulfate/ascorbate-induced mitochondria damage model, indicating that Se-GSH-HSA has potential application in medicine.
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subjects GSH
HSA
人血清白蛋白
化学改性
抗氧化活性
硒代胱氨酸
谷胱甘肽过氧化物酶
title Imprinted Human Serum Albumin with Antioxidant Activity
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