Inhibitive Determination of Heavy Metal Ions by Conductometric Nitrate Reductase Biosensor
A conductometric nitrate biosensor based on methyl viologen/Nafion/nitrate reductase（NR） interdigitated electrode for heavy metal determination was proposed.The activity of NR（EC 1.6.6.2） from Asper gillusniger（A.niger） was assayed as a function of metal concentration in the presence of Cu^2＋,Zn^2＋,...
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| Veröffentlicht in: | Chemical research in Chinese universities 2009-07, Vol.25 (4), p.443-445 |
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| creator | WANG Xue-jiang XIA Si-qing ZHAO Jian-fu ZHAO Hong-ning RENAULT Nicole Jaffrezic |
| description | A conductometric nitrate biosensor based on methyl viologen/Nafion/nitrate reductase（NR） interdigitated electrode for heavy metal determination was proposed.The activity of NR（EC 1.6.6.2） from Asper gillusniger（A.niger） was assayed as a function of metal concentration in the presence of Cu^2＋,Zn^2＋,Cd^2＋ and Pb^2＋.Parameters influencing the performance of the biosensor were optimized for the application of it in the inhibition determination of heavy metal ions.Detection limits for Cu^2＋,Zn^2＋,Cd^2＋ and Pb^2＋ were about 0.05,0.5,0.1 and 1.0 μmol/L,respectively.The results show that NR activity could not be regained after exposure to Cu^2＋,but could be partially recovered after exposure to Zn^2＋,Pb^2＋ and Cd^2＋. |
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Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Chemical research in Chinese universities</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>WANG Xue-jiang XIA Si-qing ZHAO Jian-fu ZHAO Hong-ning RENAULT Nicole Jaffrezic</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibitive Determination of Heavy Metal Ions by Conductometric Nitrate Reductase Biosensor</atitle><jtitle>Chemical research in Chinese universities</jtitle><addtitle>Chemical Research in Chinese University</addtitle><date>2009-07-25</date><risdate>2009</risdate><volume>25</volume><issue>4</issue><spage>443</spage><epage>445</epage><pages>443-445</pages><issn>1005-9040</issn><eissn>2210-3171</eissn><abstract>A conductometric nitrate biosensor based on methyl viologen/Nafion/nitrate reductase（NR） interdigitated electrode for heavy metal determination was proposed.The activity of NR（EC 1.6.6.2） from Asper gillusniger（A.niger） was assayed as a function of metal concentration in the presence of Cu^2＋,Zn^2＋,Cd^2＋ and Pb^2＋.Parameters influencing the performance of the biosensor were optimized for the application of it in the inhibition determination of heavy metal ions.Detection limits for Cu^2＋,Zn^2＋,Cd^2＋ and Pb^2＋ were about 0.05,0.5,0.1 and 1.0 μmol/L,respectively.The results show that NR activity could not be regained after exposure to Cu^2＋,but could be partially recovered after exposure to Zn^2＋,Pb^2＋ and Cd^2＋.</abstract><pub>State Key Laboratory of Pollution Control and Resources Reuse,Tongji University,Shanghai 200092,P.R.China%Laboratoire de Sciences Analytiques(UMR CNRS 5180),Universite'Claude Bernard Lyon 1,Universite'de Lyon,Vilieurbanne Cedex 69622,France</pub><tpages>3</tpages></addata></record> |
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| ispartof | Chemical research in Chinese universities, 2009-07, Vol.25 (4), p.443-445 |
| issn | 1005-9040 2210-3171 |
| language | eng |
| recordid | cdi_wanfang_journals_gdxxhxyj200904009 |
| source | Alma/SFX Local Collection |
| subjects | Nafion膜 NR活性 电导传感器 硝酸还原酶活性 重金属测定 重金属离子 金属功能 金属铜 |
| title | Inhibitive Determination of Heavy Metal Ions by Conductometric Nitrate Reductase Biosensor |
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