Direct MALDI-MS Analysis of Proteins Isolated by Liquidphase Isoelectric Focusing Electrophoresis

Direct MALDI-MS Analysis of Proteins Isolated by Liquidphase Isoelectric Focusing Electrophoresis

A liquid-phase isoelectric focusing electrophoresis system(Rotofor) was used as the prefractionation tool for the sample preparation in the MALDI-MS analysis of a protein mixture. Each fraction collected was then directly subjected to MALDI-TOF-MS analysis. By this approach, we are able to resolve t...

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Veröffentlicht in:Chemical research in Chinese universities 2005, Vol.21 (4), p.436-438
1. Verfasser: LüLei ZHANGXuan WANGZhi SUNYa-dong LIUZhi-qiang LIUShu-ying
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基质辅助激光解吸电离飞行时间质谱
液相等电子聚焦电泳
细胞色素
蛋白质
血色素
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description A liquid-phase isoelectric focusing electrophoresis system(Rotofor) was used as the prefractionation tool for the sample preparation in the MALDI-MS analysis of a protein mixture. Each fraction collected was then directly subjected to MALDI-TOF-MS analysis. By this approach, we are able to resolve two types of hemoglobins, A and C, which cannot be successfully separated by means of the traditional SDS-PAGE method.
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subjects 基质辅助激光解吸电离飞行时间质谱
液相等电子聚焦电泳
细胞色素
蛋白质
血色素
title Direct MALDI-MS Analysis of Proteins Isolated by Liquidphase Isoelectric Focusing Electrophoresis
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