菜心DNA质量对RAPD反应的影响

菜心DNA质量对RAPD反应的影响

S636.9; 采用改进的CTAB法,探讨了菜心DNA的提取质量及其对RAPD扩增效果的影响,结果表明,CTAB浓度为2%和EDTA浓度为15 mmol/L时提取的DNA质量以及DNA模板量范围在20～30 ng之间的RAPD扩增效果最好.

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Veröffentlicht in:广东农业科学 2009 (11), p.33-35
Hauptverfasser: 谭雪, 林伟君, 程小会, 林春华, 郑少薇
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container_end_page 35
container_issue 11
container_start_page 33
container_title 广东农业科学
container_volume
creator 谭雪
林伟君
程小会
林春华
郑少薇
description S636.9; 采用改进的CTAB法,探讨了菜心DNA的提取质量及其对RAPD扩增效果的影响,结果表明,CTAB浓度为2%和EDTA浓度为15 mmol/L时提取的DNA质量以及DNA模板量范围在20～30 ng之间的RAPD扩增效果最好.
doi_str_mv 10.3969/j.issn.1004-874X.2009.11.008
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title 菜心DNA质量对RAPD反应的影响
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