低能氮离子注入和紫外线复合诱变选育高产酿酒酵母

低能氮离子注入和紫外线复合诱变选育高产酿酒酵母

TL99%Q691.5; 通过离子注入联合紫外线辐射诱变选育高产酿酒酵母菌株,根据存活率及突变率确定离子注入的诱变条件为电荷18 keV,剂量400×1014cm?2,紫外线辐射60s.在该条件下,诱变获得的菌株正突变率和致死率都最高,诱变效果最好.在该条件下进行研究后获得目标菌株SC-N9015,酒精产量从14.65%提升到15.53%.多次传代实验结果显示遗传性状稳定.研究表明,通过低能氮离子注入和紫外线联合诱变选育高产酒精酵母效果显著....

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Veröffentlicht in:辐射研究与辐射工艺学报 2017, Vol.35 (2), p.44-49
Hauptverfasser: 虞龙, 姚驰亚, 吴晓菲, 李玉燕, 张娣, 虞沐尧
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container_end_page 49
container_issue 2
container_start_page 44
container_title 辐射研究与辐射工艺学报
container_volume 35
creator 虞龙
姚驰亚
吴晓菲
李玉燕
张娣
虞沐尧
description TL99%Q691.5; 通过离子注入联合紫外线辐射诱变选育高产酿酒酵母菌株,根据存活率及突变率确定离子注入的诱变条件为电荷18 keV,剂量400×1014cm?2,紫外线辐射60s.在该条件下,诱变获得的菌株正突变率和致死率都最高,诱变效果最好.在该条件下进行研究后获得目标菌株SC-N9015,酒精产量从14.65%提升到15.53%.多次传代实验结果显示遗传性状稳定.研究表明,通过低能氮离子注入和紫外线联合诱变选育高产酒精酵母效果显著.
doi_str_mv 10.11889/j.1000-3436.2017.rrj.35.020401
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title 低能氮离子注入和紫外线复合诱变选育高产酿酒酵母
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