耐促溶剂雷斯青霉菌株N~＋注入诱变选育
在甾体生物催化过程中,向发酵液中添加亲水性有机溶剂,是增加底物溶解进而提高转化效率的有效手段,但有机溶剂对于菌体普遍具有毒性。为此采用N＋注入诱变方法,对出发菌株雷斯青霉p-10-8进行改良,获得耐促溶剂菌株60 A-5-8,当投料量增大到4‰时转化率达82.61%。经过5次传代实验证明,该菌株具有较高的遗传稳定性。...
Gespeichert in:
| Veröffentlicht in: | 辐射研究与辐射工艺学报 2012, Vol.30 (2), p.97-102 |
|---|---|
| 1. Verfasser: | |
| Format: | Artikel |
| Sprache: | chi |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 102 |
|---|---|
| container_issue | 2 |
| container_start_page | 97 |
| container_title | 辐射研究与辐射工艺学报 |
| container_volume | 30 |
| creator | 刘莎 毛淑红 胡晓杰 周瑶 路福平 |
| description | 在甾体生物催化过程中,向发酵液中添加亲水性有机溶剂,是增加底物溶解进而提高转化效率的有效手段,但有机溶剂对于菌体普遍具有毒性。为此采用N＋注入诱变方法,对出发菌株雷斯青霉p-10-8进行改良,获得耐促溶剂菌株60 A-5-8,当投料量增大到4‰时转化率达82.61%。经过5次传代实验证明,该菌株具有较高的遗传稳定性。 |
| format | Article |
| fullrecord | <record><control><sourceid>wanfang_jour_chong</sourceid><recordid>TN_cdi_wanfang_journals_fsyjyfsgyxb201202007</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cqvip_id>41641771</cqvip_id><wanfj_id>fsyjyfsgyxb201202007</wanfj_id><sourcerecordid>fsyjyfsgyxb201202007</sourcerecordid><originalsourceid>FETCH-LOGICAL-c637-ec43da7f64bdab49d2dc65ba189d6e1f68004ce03bf2b6564577ef04b56ad6d83</originalsourceid><addsrcrecordid>eNpjYeA0NDAw0DU2MTbjYOAtLs5MMjA1NDY3tTQz4mQwfdEw4cn-5me7tj3tbHo5e_uzaetfzp30ck7ni_6eZwtW-dW939P9bPOKp61LX6zf-LR_xsuGzhdNm3gYWNMSc4pTeaE0N0OIm2uIs4euj7-7p7Ojj26ymbG5bmqyiXFKonmamUlSSmKSiWWKUUqymWlSoqGFZYpZqmGamYWBgUlyqoFxUppRkpmpmYmpuXlqmoFJkqlZYopZioUxN4MOxNjyxLy0xLz0-Kz80qI8oIXxacWVWZVpxemVFUlGBoZGBkYGBuZA5YoQ5ckZ-XnphZlADQVFmbmJRZXxJoZmJobm5obGAGl7YSk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>耐促溶剂雷斯青霉菌株N~＋注入诱变选育</title><source>DOAJ Directory of Open Access Journals</source><creator>刘莎 毛淑红 胡晓杰 周瑶 路福平</creator><creatorcontrib>刘莎 毛淑红 胡晓杰 周瑶 路福平</creatorcontrib><description>在甾体生物催化过程中,向发酵液中添加亲水性有机溶剂,是增加底物溶解进而提高转化效率的有效手段,但有机溶剂对于菌体普遍具有毒性。为此采用N＋注入诱变方法,对出发菌株雷斯青霉p-10-8进行改良,获得耐促溶剂菌株60 A-5-8,当投料量增大到4‰时转化率达82.61%。经过5次传代实验证明,该菌株具有较高的遗传稳定性。</description><identifier>ISSN: 1000-3436</identifier><language>chi</language><publisher>天津科技大学生物工程学院,天津,300457</publisher><subject>生物催化 ; 离子注入 ; 雷斯青霉</subject><ispartof>辐射研究与辐射工艺学报, 2012, Vol.30 (2), p.97-102</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/90700X/90700X.jpg</thumbnail><link.rule.ids>314,776,780,4010</link.rule.ids></links><search><creatorcontrib>刘莎 毛淑红 胡晓杰 周瑶 路福平</creatorcontrib><title>耐促溶剂雷斯青霉菌株N~＋注入诱变选育</title><title>辐射研究与辐射工艺学报</title><addtitle>Journal of Radiation Research and Radiation Processing</addtitle><description>在甾体生物催化过程中,向发酵液中添加亲水性有机溶剂,是增加底物溶解进而提高转化效率的有效手段,但有机溶剂对于菌体普遍具有毒性。为此采用N＋注入诱变方法,对出发菌株雷斯青霉p-10-8进行改良,获得耐促溶剂菌株60 A-5-8,当投料量增大到4‰时转化率达82.61%。经过5次传代实验证明,该菌株具有较高的遗传稳定性。</description><subject>生物催化</subject><subject>离子注入</subject><subject>雷斯青霉</subject><issn>1000-3436</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNpjYeA0NDAw0DU2MTbjYOAtLs5MMjA1NDY3tTQz4mQwfdEw4cn-5me7tj3tbHo5e_uzaetfzp30ck7ni_6eZwtW-dW939P9bPOKp61LX6zf-LR_xsuGzhdNm3gYWNMSc4pTeaE0N0OIm2uIs4euj7-7p7Ojj26ymbG5bmqyiXFKonmamUlSSmKSiWWKUUqymWlSoqGFZYpZqmGamYWBgUlyqoFxUppRkpmpmYmpuXlqmoFJkqlZYopZioUxN4MOxNjyxLy0xLz0-Kz80qI8oIXxacWVWZVpxemVFUlGBoZGBkYGBuZA5YoQ5ckZ-XnphZlADQVFmbmJRZXxJoZmJobm5obGAGl7YSk</recordid><startdate>2012</startdate><enddate>2012</enddate><creator>刘莎 毛淑红 胡晓杰 周瑶 路福平</creator><general>天津科技大学生物工程学院,天津,300457</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W92</scope><scope>~WA</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>2012</creationdate><title>耐促溶剂雷斯青霉菌株N~＋注入诱变选育</title><author>刘莎 毛淑红 胡晓杰 周瑶 路福平</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c637-ec43da7f64bdab49d2dc65ba189d6e1f68004ce03bf2b6564577ef04b56ad6d83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2012</creationdate><topic>生物催化</topic><topic>离子注入</topic><topic>雷斯青霉</topic><toplevel>online_resources</toplevel><creatorcontrib>刘莎 毛淑红 胡晓杰 周瑶 路福平</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-工程技术</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>辐射研究与辐射工艺学报</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>刘莎 毛淑红 胡晓杰 周瑶 路福平</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>耐促溶剂雷斯青霉菌株N~＋注入诱变选育</atitle><jtitle>辐射研究与辐射工艺学报</jtitle><addtitle>Journal of Radiation Research and Radiation Processing</addtitle><date>2012</date><risdate>2012</risdate><volume>30</volume><issue>2</issue><spage>97</spage><epage>102</epage><pages>97-102</pages><issn>1000-3436</issn><abstract>在甾体生物催化过程中,向发酵液中添加亲水性有机溶剂,是增加底物溶解进而提高转化效率的有效手段,但有机溶剂对于菌体普遍具有毒性。为此采用N＋注入诱变方法,对出发菌株雷斯青霉p-10-8进行改良,获得耐促溶剂菌株60 A-5-8,当投料量增大到4‰时转化率达82.61%。经过5次传代实验证明,该菌株具有较高的遗传稳定性。</abstract><pub>天津科技大学生物工程学院,天津,300457</pub><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1000-3436 |
| ispartof | 辐射研究与辐射工艺学报, 2012, Vol.30 (2), p.97-102 |
| issn | 1000-3436 |
| language | chi |
| recordid | cdi_wanfang_journals_fsyjyfsgyxb201202007 |
| source | DOAJ Directory of Open Access Journals |
| subjects | 生物催化 离子注入 雷斯青霉 |
| title | 耐促溶剂雷斯青霉菌株N~＋注入诱变选育 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T07%3A37%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-wanfang_jour_chong&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=%E8%80%90%E4%BF%83%E6%BA%B6%E5%89%82%E9%9B%B7%E6%96%AF%E9%9D%92%E9%9C%89%E8%8F%8C%E6%A0%AAN~%EF%BC%8B%E6%B3%A8%E5%85%A5%E8%AF%B1%E5%8F%98%E9%80%89%E8%82%B2&rft.jtitle=%E8%BE%90%E5%B0%84%E7%A0%94%E7%A9%B6%E4%B8%8E%E8%BE%90%E5%B0%84%E5%B7%A5%E8%89%BA%E5%AD%A6%E6%8A%A5&rft.au=%E5%88%98%E8%8E%8E%20%E6%AF%9B%E6%B7%91%E7%BA%A2%20%E8%83%A1%E6%99%93%E6%9D%B0%20%E5%91%A8%E7%91%B6%20%E8%B7%AF%E7%A6%8F%E5%B9%B3&rft.date=2012&rft.volume=30&rft.issue=2&rft.spage=97&rft.epage=102&rft.pages=97-102&rft.issn=1000-3436&rft_id=info:doi/&rft_dat=%3Cwanfang_jour_chong%3Efsyjyfsgyxb201202007%3C/wanfang_jour_chong%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rft_cqvip_id=41641771&rft_wanfj_id=fsyjyfsgyxb201202007&rfr_iscdi=true |