Improvement of Ramoplanin Production by Mutation and Medium Optimization

Improvement of Ramoplanin Production by Mutation and Medium Optimization

The aim of this work was to develop a mutation and selection procedure to select the high-yield strain, and this strain could alleviate the inhibition in the biosynthesis of ramoplanin caused by L-leucine. In this experiment, the strain Actinoplanes sp. C6-78-14 that produced 384.8 mg of total produ...

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Veröffentlicht in:The journal of Northeast Agricultural University 2008, Vol.15 (1), p.18-22
1. Verfasser: WU Xiangjin WANG Xiangjing SHI Yingzi XIANG Wensheng BAI Hua
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发酵工艺
白氨酸
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description The aim of this work was to develop a mutation and selection procedure to select the high-yield strain, and this strain could alleviate the inhibition in the biosynthesis of ramoplanin caused by L-leucine. In this experiment, the strain Actinoplanes sp. C6-78-14 that produced 384.8 mg of total productivity per liter was isolated. Its ramoplanin productivity was 4.13-fold higher than that of the parent strain A ctinoplanes sp. C2-9-45. The subculture experiments showed that the hereditary character of C6-78-14 was stable. Subsequent media studies with strain Actinoplanes sp. C6-78-14 showed that adding 0.001% FeSO4· 7H2O, 0.001% CoCl2· 6H2O and 0.001% CuSO4· 5H2O to fermentation medium respectively could enhance the ramoplanin production, and the production of each group was increased by 10%, 15% and 20% compared with the control group.
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白氨酸
title Improvement of Ramoplanin Production by Mutation and Medium Optimization
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