Analytical Behavior of Fura-2 and Its Determination of [Ca^2＋ ]i in Lymphocytes Treated with Cefotaxime
The interaction of Fura-2 with Ca^2＋ is studied using steady fluorescence technique. The effect of pH on the spectra behavior of Fura-2 in the presence of Ca^2＋ is investigated, the excitation maxima （340 nm） and the isobestic point （360 nm） for the fluorescence spectra of Fura-2 depend on pH. At di...
Gespeichert in:
| Veröffentlicht in: | 测试科学与仪器 2010, Vol.1 (4), p.395-400 |
|---|---|
| 1. Verfasser: | |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 400 |
|---|---|
| container_issue | 4 |
| container_start_page | 395 |
| container_title | 测试科学与仪器 |
| container_volume | 1 |
| creator | 双少敏 乔晓娜 王丹丹 樊丽 芦冬涛 慕丽晓 周叶红 |
| description | The interaction of Fura-2 with Ca^2＋ is studied using steady fluorescence technique. The effect of pH on the spectra behavior of Fura-2 in the presence of Ca^2＋ is investigated, the excitation maxima （340 nm） and the isobestic point （360 nm） for the fluorescence spectra of Fura-2 depend on pH. At different temperatures the apparent dissociation constants （ Kd ） of Fura-2-Ca^2＋ complex are examined, Kd is found to decrease with increasing temperatures （20 ℃, 37 ℃, 50 ℃ ） and △His calculated to be 21.16 kJ/mol by using the Van＇ t Hoff equation at pH 7.4 for all the temperatures tested. The determination of intracellular Ca^2＋ concentration （ [Ca^2＋ ] i ） in lymphocyte is developed by using Fura-2 as a fluorescence probe in the presence of Cefotaxime at 37 ℃ and pH 7.4. |
| doi_str_mv | 10.3969/j.issn.1674-8042.2010.04.21 |
| format | Article |
| fullrecord | <record><control><sourceid>wanfang_jour_chong</sourceid><recordid>TN_cdi_wanfang_journals_cskxyyq201004021</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cqvip_id>35423610</cqvip_id><wanfj_id>cskxyyq201004021</wanfj_id><sourcerecordid>cskxyyq201004021</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1181-ad83454e075294e42f652ca2145dcb6c4a16430b82e52f075931355fafb3af263</originalsourceid><addsrcrecordid>eNo9kM1OwkAcxPegiQR5h41evLTuZz-OWEVJSLzgySj5d7tLF8pWukXoM_hKvpOvYAnG0yQzv0wyg9A1JSFPo_R2FVrvXUijWAQJESxkpI-ICBk9Q4N_-wKNvLc5ITJJYxnzAbJjB1XXWgUVvtMlfNq6wbXBk10DAcPgCjxtPb7XrW421kFra3fMXzN4Zz_fX_jNYuvwrNt8lLXqWu3xvNHQ6gLvbVviTJu6hYPd6Et0bqDyevSnQ_QyeZhnT8Hs-XGajWeBojShARQJF1JoEkuWCi2YiSRTwKiQhcojJYBGgpM8YVoy01Mpp1xKAybnYFjEh-jm1LsHZ8AtF6t61_Qj_UL59aHrtsdriCCM9ujVCVVl7ZZb28M5qLWxlV5wKRiPKOG_ZYVoww</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Analytical Behavior of Fura-2 and Its Determination of [Ca^2＋ ]i in Lymphocytes Treated with Cefotaxime</title><source>Alma/SFX Local Collection</source><creator>双少敏 乔晓娜 王丹丹 樊丽 芦冬涛 慕丽晓 周叶红</creator><creatorcontrib>双少敏 乔晓娜 王丹丹 樊丽 芦冬涛 慕丽晓 周叶红</creatorcontrib><description>The interaction of Fura-2 with Ca^2＋ is studied using steady fluorescence technique. The effect of pH on the spectra behavior of Fura-2 in the presence of Ca^2＋ is investigated, the excitation maxima （340 nm） and the isobestic point （360 nm） for the fluorescence spectra of Fura-2 depend on pH. At different temperatures the apparent dissociation constants （ Kd ） of Fura-2-Ca^2＋ complex are examined, Kd is found to decrease with increasing temperatures （20 ℃, 37 ℃, 50 ℃ ） and △His calculated to be 21.16 kJ/mol by using the Van＇ t Hoff equation at pH 7.4 for all the temperatures tested. The determination of intracellular Ca^2＋ concentration （ [Ca^2＋ ] i ） in lymphocyte is developed by using Fura-2 as a fluorescence probe in the presence of Cefotaxime at 37 ℃ and pH 7.4.</description><identifier>ISSN: 1674-8042</identifier><identifier>DOI: 10.3969/j.issn.1674-8042.2010.04.21</identifier><language>eng</language><publisher>School of Chemistry and Chemical Engineering,Shanxi University,Taiyuan 030006,China%China Research Institute of Daily Chemical Industry,Taiyuan 030006,China</publisher><subject>pH值 ; 光谱行为 ; 头孢噻肟钠 ; 淋巴细胞 ; 温度上升</subject><ispartof>测试科学与仪器, 2010, Vol.1 (4), p.395-400</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1181-ad83454e075294e42f652ca2145dcb6c4a16430b82e52f075931355fafb3af263</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/70833X/70833X.jpg</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids></links><search><creatorcontrib>双少敏 乔晓娜 王丹丹 樊丽 芦冬涛 慕丽晓 周叶红</creatorcontrib><title>Analytical Behavior of Fura-2 and Its Determination of [Ca^2＋ ]i in Lymphocytes Treated with Cefotaxime</title><title>测试科学与仪器</title><addtitle>Journal of Measurement Science and Instrumentation</addtitle><description>The interaction of Fura-2 with Ca^2＋ is studied using steady fluorescence technique. The effect of pH on the spectra behavior of Fura-2 in the presence of Ca^2＋ is investigated, the excitation maxima （340 nm） and the isobestic point （360 nm） for the fluorescence spectra of Fura-2 depend on pH. At different temperatures the apparent dissociation constants （ Kd ） of Fura-2-Ca^2＋ complex are examined, Kd is found to decrease with increasing temperatures （20 ℃, 37 ℃, 50 ℃ ） and △His calculated to be 21.16 kJ/mol by using the Van＇ t Hoff equation at pH 7.4 for all the temperatures tested. The determination of intracellular Ca^2＋ concentration （ [Ca^2＋ ] i ） in lymphocyte is developed by using Fura-2 as a fluorescence probe in the presence of Cefotaxime at 37 ℃ and pH 7.4.</description><subject>pH值</subject><subject>光谱行为</subject><subject>头孢噻肟钠</subject><subject>淋巴细胞</subject><subject>温度上升</subject><issn>1674-8042</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNo9kM1OwkAcxPegiQR5h41evLTuZz-OWEVJSLzgySj5d7tLF8pWukXoM_hKvpOvYAnG0yQzv0wyg9A1JSFPo_R2FVrvXUijWAQJESxkpI-ICBk9Q4N_-wKNvLc5ITJJYxnzAbJjB1XXWgUVvtMlfNq6wbXBk10DAcPgCjxtPb7XrW421kFra3fMXzN4Zz_fX_jNYuvwrNt8lLXqWu3xvNHQ6gLvbVviTJu6hYPd6Et0bqDyevSnQ_QyeZhnT8Hs-XGajWeBojShARQJF1JoEkuWCi2YiSRTwKiQhcojJYBGgpM8YVoy01Mpp1xKAybnYFjEh-jm1LsHZ8AtF6t61_Qj_UL59aHrtsdriCCM9ujVCVVl7ZZb28M5qLWxlV5wKRiPKOG_ZYVoww</recordid><startdate>2010</startdate><enddate>2010</enddate><creator>双少敏 乔晓娜 王丹丹 樊丽 芦冬涛 慕丽晓 周叶红</creator><general>School of Chemistry and Chemical Engineering,Shanxi University,Taiyuan 030006,China%China Research Institute of Daily Chemical Industry,Taiyuan 030006,China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>~WA</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>2010</creationdate><title>Analytical Behavior of Fura-2 and Its Determination of [Ca^2＋ ]i in Lymphocytes Treated with Cefotaxime</title><author>双少敏 乔晓娜 王丹丹 樊丽 芦冬涛 慕丽晓 周叶红</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1181-ad83454e075294e42f652ca2145dcb6c4a16430b82e52f075931355fafb3af263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>pH值</topic><topic>光谱行为</topic><topic>头孢噻肟钠</topic><topic>淋巴细胞</topic><topic>温度上升</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>双少敏 乔晓娜 王丹丹 樊丽 芦冬涛 慕丽晓 周叶红</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>测试科学与仪器</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>双少敏 乔晓娜 王丹丹 樊丽 芦冬涛 慕丽晓 周叶红</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analytical Behavior of Fura-2 and Its Determination of [Ca^2＋ ]i in Lymphocytes Treated with Cefotaxime</atitle><jtitle>测试科学与仪器</jtitle><addtitle>Journal of Measurement Science and Instrumentation</addtitle><date>2010</date><risdate>2010</risdate><volume>1</volume><issue>4</issue><spage>395</spage><epage>400</epage><pages>395-400</pages><issn>1674-8042</issn><abstract>The interaction of Fura-2 with Ca^2＋ is studied using steady fluorescence technique. The effect of pH on the spectra behavior of Fura-2 in the presence of Ca^2＋ is investigated, the excitation maxima （340 nm） and the isobestic point （360 nm） for the fluorescence spectra of Fura-2 depend on pH. At different temperatures the apparent dissociation constants （ Kd ） of Fura-2-Ca^2＋ complex are examined, Kd is found to decrease with increasing temperatures （20 ℃, 37 ℃, 50 ℃ ） and △His calculated to be 21.16 kJ/mol by using the Van＇ t Hoff equation at pH 7.4 for all the temperatures tested. The determination of intracellular Ca^2＋ concentration （ [Ca^2＋ ] i ） in lymphocyte is developed by using Fura-2 as a fluorescence probe in the presence of Cefotaxime at 37 ℃ and pH 7.4.</abstract><pub>School of Chemistry and Chemical Engineering,Shanxi University,Taiyuan 030006,China%China Research Institute of Daily Chemical Industry,Taiyuan 030006,China</pub><doi>10.3969/j.issn.1674-8042.2010.04.21</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1674-8042 |
| ispartof | 测试科学与仪器, 2010, Vol.1 (4), p.395-400 |
| issn | 1674-8042 |
| language | eng |
| recordid | cdi_wanfang_journals_cskxyyq201004021 |
| source | Alma/SFX Local Collection |
| subjects | pH值 光谱行为 头孢噻肟钠 淋巴细胞 温度上升 |
| title | Analytical Behavior of Fura-2 and Its Determination of [Ca^2＋ ]i in Lymphocytes Treated with Cefotaxime |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-11T03%3A38%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-wanfang_jour_chong&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Analytical%20Behavior%20of%20Fura-2%20and%20Its%20Determination%20of%20%5BCa%5E2%EF%BC%8B%20%5Di%20in%20Lymphocytes%20Treated%20with%20Cefotaxime&rft.jtitle=%E6%B5%8B%E8%AF%95%E7%A7%91%E5%AD%A6%E4%B8%8E%E4%BB%AA%E5%99%A8&rft.au=%E5%8F%8C%E5%B0%91%E6%95%8F%20%E4%B9%94%E6%99%93%E5%A8%9C%20%E7%8E%8B%E4%B8%B9%E4%B8%B9%20%E6%A8%8A%E4%B8%BD%20%E8%8A%A6%E5%86%AC%E6%B6%9B%20%E6%85%95%E4%B8%BD%E6%99%93%20%E5%91%A8%E5%8F%B6%E7%BA%A2&rft.date=2010&rft.volume=1&rft.issue=4&rft.spage=395&rft.epage=400&rft.pages=395-400&rft.issn=1674-8042&rft_id=info:doi/10.3969/j.issn.1674-8042.2010.04.21&rft_dat=%3Cwanfang_jour_chong%3Ecskxyyq201004021%3C/wanfang_jour_chong%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rft_cqvip_id=35423610&rft_wanfj_id=cskxyyq201004021&rfr_iscdi=true |