Generation of Transgene-free Induced Pluripotent Stem Cells with Non-viral Methods
Induced pluripotent stem （iPS） cells were originally generated from mouse fibroblasts by enforced expression of Yamanaka factors （Oct3/4, Sox2, Klf4, and c-Myc）. The technique was quickly re- produced with human fibroblasts or mesenchymal stem cells. Although having been showed therapeutic po- tenti...
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| Veröffentlicht in: | Chinese medical sciences journal 2013-03, Vol.28 (1), p.50-54 |
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| creator | Wang, Tao Zhao, Hua-shan Zhang, Qiu-ling Xu, Chang-lin Liu, Chang-bai |
| description | Induced pluripotent stem （iPS） cells were originally generated from mouse fibroblasts by enforced expression of Yamanaka factors （Oct3/4, Sox2, Klf4, and c-Myc）. The technique was quickly re- produced with human fibroblasts or mesenchymal stem cells. Although having been showed therapeutic po- tential in animal models of sickle ceil anemia and Parkinson＇s disease, iPS cells generated by viral methods do not suit all the clinical applications. Various non-viral methods have appeared in recent years for application of iPS cells in cell transplantation therapy. These methods mainly include DNA vector-based approaches, transfection of mRNA, and transduction of reprogramming proteins. This review summarized these non-viral methods and compare the advantages, disadvantages, efficiency, and safety of these methods. |
| doi_str_mv | 10.1016/S1001-9294(13)60019-X |
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The technique was quickly re- produced with human fibroblasts or mesenchymal stem cells. Although having been showed therapeutic po- tential in animal models of sickle ceil anemia and Parkinson＇s disease, iPS cells generated by viral methods do not suit all the clinical applications. Various non-viral methods have appeared in recent years for application of iPS cells in cell transplantation therapy. These methods mainly include DNA vector-based approaches, transfection of mRNA, and transduction of reprogramming proteins. This review summarized these non-viral methods and compare the advantages, disadvantages, efficiency, and safety of these methods.</description><identifier>ISSN: 1001-9294</identifier><identifier>DOI: 10.1016/S1001-9294(13)60019-X</identifier><identifier>PMID: 23527808</identifier><language>eng</language><publisher>China: Elsevier B.V</publisher><subject>Animals ; c-Myc基因 ; Cellular Reprogramming ; Humans ; induced pluripotent stem cells ; Induced Pluripotent Stem Cells - physiology ; non-viral methods ; regenerative medicine ; transcription factor ; Transduction, Genetic ; Transfection ; Transgenes ; 多能干细胞 ; 成纤维细胞 ; 病毒 ; 移植治疗 ; 诱导 ; 转基因 ; 骨髓间充质干细胞</subject><ispartof>Chinese medical sciences journal, 2013-03, Vol.28 (1), p.50-54</ispartof><rights>2013 Chinese Academy Medical Sciences</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3389-5bfc14ee8da5816275b5ed0d804f1f9af7576e7cf5be7f170eeedf1c45605d9b3</citedby><cites>FETCH-LOGICAL-c3389-5bfc14ee8da5816275b5ed0d804f1f9af7576e7cf5be7f170eeedf1c45605d9b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/86798X/86798X.jpg</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23527808$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Tao</creatorcontrib><creatorcontrib>Zhao, Hua-shan</creatorcontrib><creatorcontrib>Zhang, Qiu-ling</creatorcontrib><creatorcontrib>Xu, Chang-lin</creatorcontrib><creatorcontrib>Liu, Chang-bai</creatorcontrib><title>Generation of Transgene-free Induced Pluripotent Stem Cells with Non-viral Methods</title><title>Chinese medical sciences journal</title><addtitle>Chinese Medical Sciences Journal</addtitle><description>Induced pluripotent stem （iPS） cells were originally generated from mouse fibroblasts by enforced expression of Yamanaka factors （Oct3/4, Sox2, Klf4, and c-Myc）. The technique was quickly re- produced with human fibroblasts or mesenchymal stem cells. Although having been showed therapeutic po- tential in animal models of sickle ceil anemia and Parkinson＇s disease, iPS cells generated by viral methods do not suit all the clinical applications. Various non-viral methods have appeared in recent years for application of iPS cells in cell transplantation therapy. These methods mainly include DNA vector-based approaches, transfection of mRNA, and transduction of reprogramming proteins. This review summarized these non-viral methods and compare the advantages, disadvantages, efficiency, and safety of these methods.</description><subject>Animals</subject><subject>c-Myc基因</subject><subject>Cellular Reprogramming</subject><subject>Humans</subject><subject>induced pluripotent stem cells</subject><subject>Induced Pluripotent Stem Cells - physiology</subject><subject>non-viral methods</subject><subject>regenerative medicine</subject><subject>transcription factor</subject><subject>Transduction, Genetic</subject><subject>Transfection</subject><subject>Transgenes</subject><subject>多能干细胞</subject><subject>成纤维细胞</subject><subject>病毒</subject><subject>移植治疗</subject><subject>诱导</subject><subject>转基因</subject><subject>骨髓间充质干细胞</subject><issn>1001-9294</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1O3DAUhb2gKhR4hCJ3VboI-CfOz6pCoxaQKCCgEjvLsa9nPErswU5AfXs8zMC2K8tX3_Hx_RD6SskJJbQ6vaeE0KJlbXlM-Y8qX9ricQftfYx30ZeUloSQijblZ7TLuGB1Q5o9dHcOHqIaXfA4WPwQlU_zPCpsBMCX3kwaDL7tp-hWYQQ_4vsRBjyDvk_4xY0LfB188eyi6vEfGBfBpAP0yao-weH23Ed_f_96mF0UVzfnl7Ozq0Jz3rSF6KymJUBjlGhoxWrRCTDENKS01LbK1qKuoNZWdFBbWhMAMJbqUlREmLbj--j75t0X5a3yc7kMU_S5UeohLSUwQjnJCtpMHm_IVQxPE6RRDi7pvILyEKYkKc9sLRhbo2KD6hhSimDlKrpBxX-SErmWLd9ky7XVnJNvsuVjzh1tK6ZuAPORejedgZ8bALKSZwdRJu3AZ7sugh6lCe6_Fd-2X1sEP39yeeP3llKwsmK84q8R5JvJ</recordid><startdate>201303</startdate><enddate>201303</enddate><creator>Wang, Tao</creator><creator>Zhao, Hua-shan</creator><creator>Zhang, Qiu-ling</creator><creator>Xu, Chang-lin</creator><creator>Liu, Chang-bai</creator><general>Elsevier B.V</general><general>First Clinical Medical School,China Three Georges University,Yichang 443002,China%Department of Neurosurgery,Gezhouba Central Hospital,China Three Georges University,Yichang 443002,China</general><general>Institute of Molecular Biology,China Three Georges University,Yichang 443002,China</general><general>Third Clinical Medical School,China Three Georges University,Yichang 443002,China</general><general>Three Georges University,Yichang 443002,China</general><general>Yichang City Central Hospital,China Three Georges University,Yichang 443002,China%Institute of Molecular Biology,China Three Georges University,Yichang 443002,China%Third Clinical Medical School,China Three Georges University,Yichang 443002,China First Clinical Medical School,China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>201303</creationdate><title>Generation of Transgene-free Induced Pluripotent Stem Cells with Non-viral Methods</title><author>Wang, Tao ; Zhao, Hua-shan ; Zhang, Qiu-ling ; Xu, Chang-lin ; Liu, Chang-bai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3389-5bfc14ee8da5816275b5ed0d804f1f9af7576e7cf5be7f170eeedf1c45605d9b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>c-Myc基因</topic><topic>Cellular Reprogramming</topic><topic>Humans</topic><topic>induced pluripotent stem cells</topic><topic>Induced Pluripotent Stem Cells - physiology</topic><topic>non-viral methods</topic><topic>regenerative medicine</topic><topic>transcription factor</topic><topic>Transduction, Genetic</topic><topic>Transfection</topic><topic>Transgenes</topic><topic>多能干细胞</topic><topic>成纤维细胞</topic><topic>病毒</topic><topic>移植治疗</topic><topic>诱导</topic><topic>转基因</topic><topic>骨髓间充质干细胞</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Tao</creatorcontrib><creatorcontrib>Zhao, Hua-shan</creatorcontrib><creatorcontrib>Zhang, Qiu-ling</creatorcontrib><creatorcontrib>Xu, Chang-lin</creatorcontrib><creatorcontrib>Liu, Chang-bai</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Chinese medical sciences journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Tao</au><au>Zhao, Hua-shan</au><au>Zhang, Qiu-ling</au><au>Xu, Chang-lin</au><au>Liu, Chang-bai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Generation of Transgene-free Induced Pluripotent Stem Cells with Non-viral Methods</atitle><jtitle>Chinese medical sciences journal</jtitle><addtitle>Chinese Medical Sciences Journal</addtitle><date>2013-03</date><risdate>2013</risdate><volume>28</volume><issue>1</issue><spage>50</spage><epage>54</epage><pages>50-54</pages><issn>1001-9294</issn><abstract>Induced pluripotent stem （iPS） cells were originally generated from mouse fibroblasts by enforced expression of Yamanaka factors （Oct3/4, Sox2, Klf4, and c-Myc）. The technique was quickly re- produced with human fibroblasts or mesenchymal stem cells. Although having been showed therapeutic po- tential in animal models of sickle ceil anemia and Parkinson＇s disease, iPS cells generated by viral methods do not suit all the clinical applications. Various non-viral methods have appeared in recent years for application of iPS cells in cell transplantation therapy. These methods mainly include DNA vector-based approaches, transfection of mRNA, and transduction of reprogramming proteins. This review summarized these non-viral methods and compare the advantages, disadvantages, efficiency, and safety of these methods.</abstract><cop>China</cop><pub>Elsevier B.V</pub><pmid>23527808</pmid><doi>10.1016/S1001-9294(13)60019-X</doi><tpages>5</tpages></addata></record> |
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| subjects | Animals c-Myc基因 Cellular Reprogramming Humans induced pluripotent stem cells Induced Pluripotent Stem Cells - physiology non-viral methods regenerative medicine transcription factor Transduction, Genetic Transfection Transgenes 多能干细胞 成纤维细胞 病毒 移植治疗 诱导 转基因 骨髓间充质干细胞 |
| title | Generation of Transgene-free Induced Pluripotent Stem Cells with Non-viral Methods |
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