STUDY ON MODIFIED COLD STORAGE METHOD OF RAT LIVERS WITH SELF-MADE HYD SOLUTION
Objective. To investigate the cold preservation effect of rat livers by modified storage method with self-made HYD solution. Methods. The modified method was that the vascular bed of tat livers was expanded with an additional 20 to 40ml self-made HYD selution/100g liver. After removing the liver, th...
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| Veröffentlicht in: | Chinese medical sciences journal 2000-09, Vol.15 (3), p.191-194 |
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| creator | 孙备 姜洪池 乔海泉 朴大勋 孙金圣 朱世军 王孝铭 |
| description | Objective. To investigate the cold preservation effect of rat livers by modified storage method with self-made HYD solution. Methods. The modified method was that the vascular bed of tat livers was expanded with an additional 20 to 40ml self-made HYD selution/100g liver. After removing the liver, the extra HYD solution expressed as % liver weight was entrapped via portal infusion by tying off the supra-and infra hepatic inferior vena cava. According to the amount of extra HYD solution, 40 rats were randomly divided into four groups including: control group with conventional storage method, 20% group, 30% group and 40% group. The preservation effect of modified storage method with that of conventional storage method by using isolated perfused tat liver model was compared. Results. Bile production and all the indices of hepatic microcirculation including portal perfusion pressure, en-dothelin-1 in the effluent, trypan blue distribution time and histology in modified method groups were significantly su-perior to those in control group ( P < 0.05). The liver enzymes in 30% group were markedly lower than those in con-trol group (P< 0.05). The preservation effect of rat liver in 30% group was the best atrmng the modified methodgroups. Conclusion. The modified cold storage method is effective and may have potential for clinical apphcation for liver preservation. |
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To investigate the cold preservation effect of rat livers by modified storage method with self-made HYD solution. Methods. The modified method was that the vascular bed of tat livers was expanded with an additional 20 to 40ml self-made HYD selution/100g liver. After removing the liver, the extra HYD solution expressed as % liver weight was entrapped via portal infusion by tying off the supra-and infra hepatic inferior vena cava. According to the amount of extra HYD solution, 40 rats were randomly divided into four groups including: control group with conventional storage method, 20% group, 30% group and 40% group. The preservation effect of modified storage method with that of conventional storage method by using isolated perfused tat liver model was compared. Results. Bile production and all the indices of hepatic microcirculation including portal perfusion pressure, en-dothelin-1 in the effluent, trypan blue distribution time and histology in modified method groups were significantly su-perior to those in control group ( P < 0.05). The liver enzymes in 30% group were markedly lower than those in con-trol group (P< 0.05). The preservation effect of rat liver in 30% group was the best atrmng the modified methodgroups. Conclusion. The modified cold storage method is effective and may have potential for clinical apphcation for liver preservation.</description><identifier>ISSN: 1001-9294</identifier><identifier>PMID: 12903784</identifier><language>eng</language><publisher>China: Department of General Surgery, First Clinical Hospital,Harbin Medical University, Harbin 150001</publisher><subject>Alanine Transaminase - metabolism ; Animals ; Aspartate Aminotransferases - metabolism ; Bile - metabolism ; Cold Temperature ; Endothelin-1 - metabolism ; L-Lactate Dehydrogenase - metabolism ; Liver - anatomy & histology ; Liver - drug effects ; Liver - metabolism ; Male ; Organ Preservation - methods ; Organ Preservation Solutions - pharmacology ; Perfusion ; Portal Vein - physiology ; Pressure ; Rats ; Rats, Wistar ; 冷冻贮藏 ; 贮藏方法</subject><ispartof>Chinese medical sciences journal, 2000-09, Vol.15 (3), p.191-194</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/86798X/86798X.jpg</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12903784$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>孙备 姜洪池 乔海泉 朴大勋 孙金圣 朱世军 王孝铭</creatorcontrib><title>STUDY ON MODIFIED COLD STORAGE METHOD OF RAT LIVERS WITH SELF-MADE HYD SOLUTION</title><title>Chinese medical sciences journal</title><addtitle>Chinese Medical Sciences Journal</addtitle><description>Objective. To investigate the cold preservation effect of rat livers by modified storage method with self-made HYD solution. Methods. The modified method was that the vascular bed of tat livers was expanded with an additional 20 to 40ml self-made HYD selution/100g liver. After removing the liver, the extra HYD solution expressed as % liver weight was entrapped via portal infusion by tying off the supra-and infra hepatic inferior vena cava. According to the amount of extra HYD solution, 40 rats were randomly divided into four groups including: control group with conventional storage method, 20% group, 30% group and 40% group. The preservation effect of modified storage method with that of conventional storage method by using isolated perfused tat liver model was compared. Results. Bile production and all the indices of hepatic microcirculation including portal perfusion pressure, en-dothelin-1 in the effluent, trypan blue distribution time and histology in modified method groups were significantly su-perior to those in control group ( P < 0.05). The liver enzymes in 30% group were markedly lower than those in con-trol group (P< 0.05). The preservation effect of rat liver in 30% group was the best atrmng the modified methodgroups. Conclusion. The modified cold storage method is effective and may have potential for clinical apphcation for liver preservation.</description><subject>Alanine Transaminase - metabolism</subject><subject>Animals</subject><subject>Aspartate Aminotransferases - metabolism</subject><subject>Bile - metabolism</subject><subject>Cold Temperature</subject><subject>Endothelin-1 - metabolism</subject><subject>L-Lactate Dehydrogenase - metabolism</subject><subject>Liver - anatomy & histology</subject><subject>Liver - drug effects</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Organ Preservation - methods</subject><subject>Organ Preservation Solutions - pharmacology</subject><subject>Perfusion</subject><subject>Portal Vein - physiology</subject><subject>Pressure</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>冷冻贮藏</subject><subject>贮藏方法</subject><issn>1001-9294</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo10EtPg0AUBeBZaGyt_gUzbnRFcufRAktSoJDQTlKopqvJMB0qlUcFifHfS9K6upsv5-TcGzQlAMRyqcsn6L7vTwCwIA6_QxNCXWC2w6dIpNnO32OxwWvhx2Ec-HgpEh-nmdh6qwCvgywSPhYh3noZTuK3YJvi9ziLcBokobX2_ABH-9GLZJfFYvOAbgtV9ebxemdoFwbZMrISsYqXXmJp4gC3WK4UI1AobVxq55wbpjTPgWuXOcQlzDWEHhhVhALlCwAnZweea8WKhV0wxmbo9ZL7o5pCNUd5aoeuGRulrvuTNHQcCwwIH-XLRZ679msw_besy16bqlKNaYde2jDnnI94hp6ucMhrc5DnrqxV9yv_nzWC5wvQH21z_CrH1lzpz6KsjHRsAnPmsD-p3WZj</recordid><startdate>200009</startdate><enddate>200009</enddate><creator>孙备 姜洪池 乔海泉 朴大勋 孙金圣 朱世军 王孝铭</creator><general>Department of General Surgery, First Clinical Hospital,Harbin Medical University, Harbin 150001</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>200009</creationdate><title>STUDY ON MODIFIED COLD STORAGE METHOD OF RAT LIVERS WITH SELF-MADE HYD SOLUTION</title><author>孙备 姜洪池 乔海泉 朴大勋 孙金圣 朱世军 王孝铭</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1804-3baa310face927b44e3ac4b04c93819139e12d32a120246008b3d4bca3f67f333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Alanine Transaminase - metabolism</topic><topic>Animals</topic><topic>Aspartate Aminotransferases - metabolism</topic><topic>Bile - metabolism</topic><topic>Cold Temperature</topic><topic>Endothelin-1 - metabolism</topic><topic>L-Lactate Dehydrogenase - metabolism</topic><topic>Liver - anatomy & histology</topic><topic>Liver - drug effects</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Organ Preservation - methods</topic><topic>Organ Preservation Solutions - pharmacology</topic><topic>Perfusion</topic><topic>Portal Vein - physiology</topic><topic>Pressure</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>冷冻贮藏</topic><topic>贮藏方法</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>孙备 姜洪池 乔海泉 朴大勋 孙金圣 朱世军 王孝铭</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Chinese medical sciences journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>孙备 姜洪池 乔海泉 朴大勋 孙金圣 朱世军 王孝铭</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>STUDY ON MODIFIED COLD STORAGE METHOD OF RAT LIVERS WITH SELF-MADE HYD SOLUTION</atitle><jtitle>Chinese medical sciences journal</jtitle><addtitle>Chinese Medical Sciences Journal</addtitle><date>2000-09</date><risdate>2000</risdate><volume>15</volume><issue>3</issue><spage>191</spage><epage>194</epage><pages>191-194</pages><issn>1001-9294</issn><abstract>Objective. To investigate the cold preservation effect of rat livers by modified storage method with self-made HYD solution. Methods. The modified method was that the vascular bed of tat livers was expanded with an additional 20 to 40ml self-made HYD selution/100g liver. After removing the liver, the extra HYD solution expressed as % liver weight was entrapped via portal infusion by tying off the supra-and infra hepatic inferior vena cava. According to the amount of extra HYD solution, 40 rats were randomly divided into four groups including: control group with conventional storage method, 20% group, 30% group and 40% group. The preservation effect of modified storage method with that of conventional storage method by using isolated perfused tat liver model was compared. Results. Bile production and all the indices of hepatic microcirculation including portal perfusion pressure, en-dothelin-1 in the effluent, trypan blue distribution time and histology in modified method groups were significantly su-perior to those in control group ( P < 0.05). The liver enzymes in 30% group were markedly lower than those in con-trol group (P< 0.05). The preservation effect of rat liver in 30% group was the best atrmng the modified methodgroups. Conclusion. The modified cold storage method is effective and may have potential for clinical apphcation for liver preservation.</abstract><cop>China</cop><pub>Department of General Surgery, First Clinical Hospital,Harbin Medical University, Harbin 150001</pub><pmid>12903784</pmid><tpages>4</tpages></addata></record> |
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| subjects | Alanine Transaminase - metabolism Animals Aspartate Aminotransferases - metabolism Bile - metabolism Cold Temperature Endothelin-1 - metabolism L-Lactate Dehydrogenase - metabolism Liver - anatomy & histology Liver - drug effects Liver - metabolism Male Organ Preservation - methods Organ Preservation Solutions - pharmacology Perfusion Portal Vein - physiology Pressure Rats Rats, Wistar 冷冻贮藏 贮藏方法 |
| title | STUDY ON MODIFIED COLD STORAGE METHOD OF RAT LIVERS WITH SELF-MADE HYD SOLUTION |
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