Genetic Diversity, Antimicrobial Resistance, and Virulence Genes of Aeromonas Isolates from Clinical Patients, Tap Water Systems, and Food
This study aimed to evaluate the genetic diversity, virulence, and antimicrobial resistance of Aeromonas isolates from clinical patients, tap water systems, and food. Ninety Aeromonas isolates were obtained from Ma’anshan, Anhui province, China, and subjected to multi-locus sequence typing (MLST) wi...
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| Veröffentlicht in: | Biomedical and environmental sciences 2020-06, Vol.33 (6), p.385-395 |
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| creator | MENG, Shuang WANG, Yong Lu LIU, Chen Geng YANG, Jing YUAN, Min BAI, Xiang Ning JIN, Dong LIANG, Jun Rong CUI, Zhi Gang LI, Juan |
| description | This study aimed to evaluate the genetic diversity, virulence, and antimicrobial resistance of Aeromonas isolates from clinical patients, tap water systems, and food.
Ninety Aeromonas isolates were obtained from Ma’anshan, Anhui province, China, and subjected to multi-locus sequence typing (MLST) with six housekeeping genes. Their taxonomy was investigated using concatenated gyrB-cpn60 sequences, while their resistance to 12 antibiotics was evaluated. Ten putative virulence factors and several resistance genes were identified by PCR and sequencing.
The 90 Aeromonas isolates were divided into 84 sequence types, 80 of which were novel, indicating high genetic diversity. The Aeromonas isolates were classified into eight different species. PCR assays identified virulence genes in the isolates, with the enterotoxin and hemolysin genes act, aerA, alt, and ast found in 47 (52.2%), 13 (14.4%), 22 (24.4%), and 12 (13.3%) of the isolates, respectively. The majority of the isolates (≥ 90%) were susceptible to aztreonam, imipenem, cefepime, chloramphenicol, gentamicin, tetracycline, and ciprofloxacin. However, several resistance genes were detected in the isolates, as well as a new mcr-3 variant.
Sequence type, virulence properties, and antibiotic resistance vary in Aeromonas isolates from clinical patients, tap water systems, and food. |
| doi_str_mv | 10.3967/bes2020.053 |
| format | Article |
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Ninety Aeromonas isolates were obtained from Ma’anshan, Anhui province, China, and subjected to multi-locus sequence typing (MLST) with six housekeeping genes. Their taxonomy was investigated using concatenated gyrB-cpn60 sequences, while their resistance to 12 antibiotics was evaluated. Ten putative virulence factors and several resistance genes were identified by PCR and sequencing.
The 90 Aeromonas isolates were divided into 84 sequence types, 80 of which were novel, indicating high genetic diversity. The Aeromonas isolates were classified into eight different species. PCR assays identified virulence genes in the isolates, with the enterotoxin and hemolysin genes act, aerA, alt, and ast found in 47 (52.2%), 13 (14.4%), 22 (24.4%), and 12 (13.3%) of the isolates, respectively. The majority of the isolates (≥ 90%) were susceptible to aztreonam, imipenem, cefepime, chloramphenicol, gentamicin, tetracycline, and ciprofloxacin. However, several resistance genes were detected in the isolates, as well as a new mcr-3 variant.
Sequence type, virulence properties, and antibiotic resistance vary in Aeromonas isolates from clinical patients, tap water systems, and food.</description><identifier>ISSN: 0895-3988</identifier><identifier>EISSN: 2214-0190</identifier><identifier>DOI: 10.3967/bes2020.053</identifier><identifier>PMID: 32641201</identifier><language>eng</language><publisher>China: Elsevier B.V</publisher><subject>Aeromonas ; Aeromonas - drug effects ; Aeromonas - genetics ; Aeromonas - isolation & purification ; Aeromonas - pathogenicity ; Anti-Bacterial Agents - pharmacology ; Antimicrobial resistance gene ; China ; Drinking Water - microbiology ; Drug Resistance, Bacterial ; Food Microbiology ; Genetic Variation ; Gram-Negative Bacterial Infections - microbiology ; Multi-locus sequence typing ; Multidrug resistance ; Species Specificity ; Virulence ; Virulence gene</subject><ispartof>Biomedical and environmental sciences, 2020-06, Vol.33 (6), p.385-395</ispartof><rights>2020 The Editorial Board of Biomedical and Environmental Sciences</rights><rights>Copyright © 2020 The Editorial Board of Biomedical and Environmental Sciences. Published by China CDC. All rights reserved.</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/bes/bes.jpg</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.3967/bes2020.053$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32641201$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MENG, Shuang</creatorcontrib><creatorcontrib>WANG, Yong Lu</creatorcontrib><creatorcontrib>LIU, Chen Geng</creatorcontrib><creatorcontrib>YANG, Jing</creatorcontrib><creatorcontrib>YUAN, Min</creatorcontrib><creatorcontrib>BAI, Xiang Ning</creatorcontrib><creatorcontrib>JIN, Dong</creatorcontrib><creatorcontrib>LIANG, Jun Rong</creatorcontrib><creatorcontrib>CUI, Zhi Gang</creatorcontrib><creatorcontrib>LI, Juan</creatorcontrib><title>Genetic Diversity, Antimicrobial Resistance, and Virulence Genes of Aeromonas Isolates from Clinical Patients, Tap Water Systems, and Food</title><title>Biomedical and environmental sciences</title><addtitle>Biomed Environ Sci</addtitle><description>This study aimed to evaluate the genetic diversity, virulence, and antimicrobial resistance of Aeromonas isolates from clinical patients, tap water systems, and food.
Ninety Aeromonas isolates were obtained from Ma’anshan, Anhui province, China, and subjected to multi-locus sequence typing (MLST) with six housekeeping genes. Their taxonomy was investigated using concatenated gyrB-cpn60 sequences, while their resistance to 12 antibiotics was evaluated. Ten putative virulence factors and several resistance genes were identified by PCR and sequencing.
The 90 Aeromonas isolates were divided into 84 sequence types, 80 of which were novel, indicating high genetic diversity. The Aeromonas isolates were classified into eight different species. PCR assays identified virulence genes in the isolates, with the enterotoxin and hemolysin genes act, aerA, alt, and ast found in 47 (52.2%), 13 (14.4%), 22 (24.4%), and 12 (13.3%) of the isolates, respectively. The majority of the isolates (≥ 90%) were susceptible to aztreonam, imipenem, cefepime, chloramphenicol, gentamicin, tetracycline, and ciprofloxacin. However, several resistance genes were detected in the isolates, as well as a new mcr-3 variant.
Sequence type, virulence properties, and antibiotic resistance vary in Aeromonas isolates from clinical patients, tap water systems, and food.</description><subject>Aeromonas</subject><subject>Aeromonas - drug effects</subject><subject>Aeromonas - genetics</subject><subject>Aeromonas - isolation & purification</subject><subject>Aeromonas - pathogenicity</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antimicrobial resistance gene</subject><subject>China</subject><subject>Drinking Water - microbiology</subject><subject>Drug Resistance, Bacterial</subject><subject>Food Microbiology</subject><subject>Genetic Variation</subject><subject>Gram-Negative Bacterial Infections - microbiology</subject><subject>Multi-locus sequence typing</subject><subject>Multidrug resistance</subject><subject>Species Specificity</subject><subject>Virulence</subject><subject>Virulence gene</subject><issn>0895-3988</issn><issn>2214-0190</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kU1rGzEQhkVpaZykp96LDj1605G0WmuPxmk-INDS5uMotNJsUdiVjCSn-C_0V1fGyWmYl2feGd4h5DODC9F3q28DZg4cLkCKd2TBOWsbYD28JwtQvWxEr9QJOc35GaBlfas-khPBu5ZxYAvy7xoDFm_ppX_BlH3ZL-k6FD97m-LgzUR_Yfa5mGBxSU1w9NGn3YS1pYfRTONI15jiHIPJ9DbHyZSqjlWhm8kHb6vHT1M8hpKX9N5s6VMlEv29zwXnfDS9itGdkw-jmTJ-eq1n5OHq-_3mprn7cX27Wd81yFVXmnbgQiATo3JMWimcW7Gul86gZZKvVqAG2w9OCQ4wSGUY78QolZRm7CQIKc7I16PvXxNGE_7o57hLoW7Ur0lCB8Aq9uWIbXfDjE5vk59N2uu37CogjwDWY188Jp2tPwTjfEJbtIteM9CHH7056_oj8R8R5oIB</recordid><startdate>20200601</startdate><enddate>20200601</enddate><creator>MENG, Shuang</creator><creator>WANG, Yong Lu</creator><creator>LIU, Chen Geng</creator><creator>YANG, Jing</creator><creator>YUAN, Min</creator><creator>BAI, Xiang Ning</creator><creator>JIN, Dong</creator><creator>LIANG, Jun Rong</creator><creator>CUI, Zhi Gang</creator><creator>LI, Juan</creator><general>Elsevier B.V</general><general>State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 10026, China</general><general>Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang 310058, Hangzhou, China%Ma'anshan Center for Disease Control and Prevention, Ma'anshan 243031, Anhui, China%Clinical Laboratory of Xuanwu Hospital, Captital Medcial University, Beijing 100053, China</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20200601</creationdate><title>Genetic Diversity, Antimicrobial Resistance, and Virulence Genes of Aeromonas Isolates from Clinical Patients, Tap Water Systems, and Food</title><author>MENG, Shuang ; WANG, Yong Lu ; LIU, Chen Geng ; YANG, Jing ; YUAN, Min ; BAI, Xiang Ning ; JIN, Dong ; LIANG, Jun Rong ; CUI, Zhi Gang ; LI, Juan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e286t-4b233e13f8d15c53dd71695daec1527708bc9bd83200b58a1263f5855af650353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Aeromonas</topic><topic>Aeromonas - drug effects</topic><topic>Aeromonas - genetics</topic><topic>Aeromonas - isolation & purification</topic><topic>Aeromonas - pathogenicity</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antimicrobial resistance gene</topic><topic>China</topic><topic>Drinking Water - microbiology</topic><topic>Drug Resistance, Bacterial</topic><topic>Food Microbiology</topic><topic>Genetic Variation</topic><topic>Gram-Negative Bacterial Infections - microbiology</topic><topic>Multi-locus sequence typing</topic><topic>Multidrug resistance</topic><topic>Species Specificity</topic><topic>Virulence</topic><topic>Virulence gene</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MENG, Shuang</creatorcontrib><creatorcontrib>WANG, Yong Lu</creatorcontrib><creatorcontrib>LIU, Chen Geng</creatorcontrib><creatorcontrib>YANG, Jing</creatorcontrib><creatorcontrib>YUAN, Min</creatorcontrib><creatorcontrib>BAI, Xiang Ning</creatorcontrib><creatorcontrib>JIN, Dong</creatorcontrib><creatorcontrib>LIANG, Jun Rong</creatorcontrib><creatorcontrib>CUI, Zhi Gang</creatorcontrib><creatorcontrib>LI, Juan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Biomedical and environmental sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MENG, Shuang</au><au>WANG, Yong Lu</au><au>LIU, Chen Geng</au><au>YANG, Jing</au><au>YUAN, Min</au><au>BAI, Xiang Ning</au><au>JIN, Dong</au><au>LIANG, Jun Rong</au><au>CUI, Zhi Gang</au><au>LI, Juan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic Diversity, Antimicrobial Resistance, and Virulence Genes of Aeromonas Isolates from Clinical Patients, Tap Water Systems, and Food</atitle><jtitle>Biomedical and environmental sciences</jtitle><addtitle>Biomed Environ Sci</addtitle><date>2020-06-01</date><risdate>2020</risdate><volume>33</volume><issue>6</issue><spage>385</spage><epage>395</epage><pages>385-395</pages><issn>0895-3988</issn><eissn>2214-0190</eissn><abstract>This study aimed to evaluate the genetic diversity, virulence, and antimicrobial resistance of Aeromonas isolates from clinical patients, tap water systems, and food.
Ninety Aeromonas isolates were obtained from Ma’anshan, Anhui province, China, and subjected to multi-locus sequence typing (MLST) with six housekeeping genes. Their taxonomy was investigated using concatenated gyrB-cpn60 sequences, while their resistance to 12 antibiotics was evaluated. Ten putative virulence factors and several resistance genes were identified by PCR and sequencing.
The 90 Aeromonas isolates were divided into 84 sequence types, 80 of which were novel, indicating high genetic diversity. The Aeromonas isolates were classified into eight different species. PCR assays identified virulence genes in the isolates, with the enterotoxin and hemolysin genes act, aerA, alt, and ast found in 47 (52.2%), 13 (14.4%), 22 (24.4%), and 12 (13.3%) of the isolates, respectively. The majority of the isolates (≥ 90%) were susceptible to aztreonam, imipenem, cefepime, chloramphenicol, gentamicin, tetracycline, and ciprofloxacin. However, several resistance genes were detected in the isolates, as well as a new mcr-3 variant.
Sequence type, virulence properties, and antibiotic resistance vary in Aeromonas isolates from clinical patients, tap water systems, and food.</abstract><cop>China</cop><pub>Elsevier B.V</pub><pmid>32641201</pmid><doi>10.3967/bes2020.053</doi><tpages>11</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Aeromonas Aeromonas - drug effects Aeromonas - genetics Aeromonas - isolation & purification Aeromonas - pathogenicity Anti-Bacterial Agents - pharmacology Antimicrobial resistance gene China Drinking Water - microbiology Drug Resistance, Bacterial Food Microbiology Genetic Variation Gram-Negative Bacterial Infections - microbiology Multi-locus sequence typing Multidrug resistance Species Specificity Virulence Virulence gene |
| title | Genetic Diversity, Antimicrobial Resistance, and Virulence Genes of Aeromonas Isolates from Clinical Patients, Tap Water Systems, and Food |
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