Evaluation of the New Automatic Mycob.T Stainer and Scanner for Detecting Acid-fast Bacilli in China
To validate the performance of Mycob. T Stainer and Scanner (MTSS) for detecting acid-fast bacilli (AFB). A total of 3,816 sputum samples from 1,515 tuberculosis (TB) suspects were tested at the Anhui Provincial Chest Hospital and the Linyi People's Hospital from April-August, 2016. Each specim...
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| Veröffentlicht in: | Biomedical and environmental sciences 2018-08, Vol.31 (8), p.572-578 |
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| container_title | Biomedical and environmental sciences |
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| creator | LI, Qiang ZHAO, Yan Lin WANG, Qing DENG, Yun Feng BAO, Xun Di YANG, Hong Guo OU, Xi Chao ZHAO, Bing ZHANG, Jing Bo |
| description | To validate the performance of Mycob. T Stainer and Scanner (MTSS) for detecting acid-fast bacilli (AFB).
A total of 3,816 sputum samples from 1,515 tuberculosis (TB) suspects were tested at the Anhui Provincial Chest Hospital and the Linyi People's Hospital from April-August, 2016. Each specimen was placed on two smear slides. One slide was stained by the ziehl-neelsen (ZN) method to be read by conventional microscopy (CM). The other slide was stained and scanned by MTSS. All specimens were decontaminated with 4% NaOH, and then inoculated into solid culture. The performance of MTSS was assessed.
MTSS produced higher average positivity rate (27.96%) as compared with the CM (26.83%). The overall sensitivity and specificity of MTSS were 78.9% and 93.9%, respectively. The sensitivity and specificity of CM was 77.4% and 95.0%, respectively.
MTSS exhibited a favorable performance in the detection of AFB. It may be an alternative to CM for screening TB. |
| doi_str_mv | 10.3967/bes2018.078 |
| format | Article |
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A total of 3,816 sputum samples from 1,515 tuberculosis (TB) suspects were tested at the Anhui Provincial Chest Hospital and the Linyi People's Hospital from April-August, 2016. Each specimen was placed on two smear slides. One slide was stained by the ziehl-neelsen (ZN) method to be read by conventional microscopy (CM). The other slide was stained and scanned by MTSS. All specimens were decontaminated with 4% NaOH, and then inoculated into solid culture. The performance of MTSS was assessed.
MTSS produced higher average positivity rate (27.96%) as compared with the CM (26.83%). The overall sensitivity and specificity of MTSS were 78.9% and 93.9%, respectively. The sensitivity and specificity of CM was 77.4% and 95.0%, respectively.
MTSS exhibited a favorable performance in the detection of AFB. It may be an alternative to CM for screening TB.</description><identifier>ISSN: 0895-3988</identifier><identifier>EISSN: 2214-0190</identifier><identifier>DOI: 10.3967/bes2018.078</identifier><identifier>PMID: 30231961</identifier><language>eng</language><publisher>China: Elsevier B.V</publisher><subject>Automatic ; China ; Hospitals ; Mycobacterium tuberculosis ; Scanner ; Sensitivity and Specificity ; Sputum - microbiology ; Stainer ; Staining and Labeling - methods ; Tuberculosis ; Tuberculosis, Pulmonary - diagnosis</subject><ispartof>Biomedical and environmental sciences, 2018-08, Vol.31 (8), p.572-578</ispartof><rights>2018 The Editorial Board of Biomedical and Environmental Sciences</rights><rights>Copyright © 2018 The Editorial Board of Biomedical and Environmental Sciences. Published by China CDC. All rights reserved.</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/bes/bes.jpg</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0895398819300224$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30231961$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LI, Qiang</creatorcontrib><creatorcontrib>ZHAO, Yan Lin</creatorcontrib><creatorcontrib>WANG, Qing</creatorcontrib><creatorcontrib>DENG, Yun Feng</creatorcontrib><creatorcontrib>BAO, Xun Di</creatorcontrib><creatorcontrib>YANG, Hong Guo</creatorcontrib><creatorcontrib>OU, Xi Chao</creatorcontrib><creatorcontrib>ZHAO, Bing</creatorcontrib><creatorcontrib>ZHANG, Jing Bo</creatorcontrib><title>Evaluation of the New Automatic Mycob.T Stainer and Scanner for Detecting Acid-fast Bacilli in China</title><title>Biomedical and environmental sciences</title><addtitle>Biomed Environ Sci</addtitle><description>To validate the performance of Mycob. T Stainer and Scanner (MTSS) for detecting acid-fast bacilli (AFB).
A total of 3,816 sputum samples from 1,515 tuberculosis (TB) suspects were tested at the Anhui Provincial Chest Hospital and the Linyi People's Hospital from April-August, 2016. Each specimen was placed on two smear slides. One slide was stained by the ziehl-neelsen (ZN) method to be read by conventional microscopy (CM). The other slide was stained and scanned by MTSS. All specimens were decontaminated with 4% NaOH, and then inoculated into solid culture. The performance of MTSS was assessed.
MTSS produced higher average positivity rate (27.96%) as compared with the CM (26.83%). The overall sensitivity and specificity of MTSS were 78.9% and 93.9%, respectively. The sensitivity and specificity of CM was 77.4% and 95.0%, respectively.
MTSS exhibited a favorable performance in the detection of AFB. It may be an alternative to CM for screening TB.</description><subject>Automatic</subject><subject>China</subject><subject>Hospitals</subject><subject>Mycobacterium tuberculosis</subject><subject>Scanner</subject><subject>Sensitivity and Specificity</subject><subject>Sputum - microbiology</subject><subject>Stainer</subject><subject>Staining and Labeling - methods</subject><subject>Tuberculosis</subject><subject>Tuberculosis, Pulmonary - diagnosis</subject><issn>0895-3988</issn><issn>2214-0190</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kT1vGzEMhoWiQeO4nboXGjp0OYfSyXfS6LpJWiBth6SzwNNHouAspSddgvz7yLCzkATxgOT7kpDPDFat6vrzwWUOTK6gl-_IgnMmGmAK3pMFSLVuWiXlKTnL-QFAMCXkB3LaAm-Z6tiC2IsnHGcsIUWaPC33jv5xz3Qzl7SrXUN_v5g0rG7pTcEQ3UQxWnpjMO5rnyb6wxVnSoh3dGOCbTzmQr-jCeMYaIh0ex8ifiQnHsfsPh3zkvy7vLjd_myu_1792m6uG8dlVxouJHjJEQ1Hx6ztYehU14LvgEvjRbcWTnjEfZSDVRx74H0rOEgBAxftknw9zH3G6DHe6Yc0T7Fu1EePQEJVviTfDtjjlP7PLhe9C9m4ccTo0px1dVBJXhezin45ovOwc1Y_TmGH04t-M7AC6wPgqq6n4CadTXDROBum6ou2KWgGev-otyN0fVT7Ci68gZM</recordid><startdate>20180801</startdate><enddate>20180801</enddate><creator>LI, Qiang</creator><creator>ZHAO, Yan Lin</creator><creator>WANG, Qing</creator><creator>DENG, Yun Feng</creator><creator>BAO, Xun Di</creator><creator>YANG, Hong Guo</creator><creator>OU, Xi Chao</creator><creator>ZHAO, Bing</creator><creator>ZHANG, Jing Bo</creator><general>Elsevier B.V</general><general>Beijing Physical Examination Center,Beijing 100077,China%National Center for Tuberculosis Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China%Anhui Provincial Chest Hospital,Hefei 230022,Anhui,China%Shandong Provincial Chest Hospital,Jinan 250013,Shandong,China%Linyi People's Hospital,Linyi 276003,Shandong,China</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20180801</creationdate><title>Evaluation of the New Automatic Mycob.T Stainer and Scanner for Detecting Acid-fast Bacilli in China</title><author>LI, Qiang ; ZHAO, Yan Lin ; WANG, Qing ; DENG, Yun Feng ; BAO, Xun Di ; YANG, Hong Guo ; OU, Xi Chao ; ZHAO, Bing ; ZHANG, Jing Bo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e286t-2480f82aac2ae1dd70b69630f6028cf4654e4faa4e4f8bd92a70273420840b243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Automatic</topic><topic>China</topic><topic>Hospitals</topic><topic>Mycobacterium tuberculosis</topic><topic>Scanner</topic><topic>Sensitivity and Specificity</topic><topic>Sputum - microbiology</topic><topic>Stainer</topic><topic>Staining and Labeling - methods</topic><topic>Tuberculosis</topic><topic>Tuberculosis, Pulmonary - diagnosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LI, Qiang</creatorcontrib><creatorcontrib>ZHAO, Yan Lin</creatorcontrib><creatorcontrib>WANG, Qing</creatorcontrib><creatorcontrib>DENG, Yun Feng</creatorcontrib><creatorcontrib>BAO, Xun Di</creatorcontrib><creatorcontrib>YANG, Hong Guo</creatorcontrib><creatorcontrib>OU, Xi Chao</creatorcontrib><creatorcontrib>ZHAO, Bing</creatorcontrib><creatorcontrib>ZHANG, Jing Bo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Biomedical and environmental sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LI, Qiang</au><au>ZHAO, Yan Lin</au><au>WANG, Qing</au><au>DENG, Yun Feng</au><au>BAO, Xun Di</au><au>YANG, Hong Guo</au><au>OU, Xi Chao</au><au>ZHAO, Bing</au><au>ZHANG, Jing Bo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of the New Automatic Mycob.T Stainer and Scanner for Detecting Acid-fast Bacilli in China</atitle><jtitle>Biomedical and environmental sciences</jtitle><addtitle>Biomed Environ Sci</addtitle><date>2018-08-01</date><risdate>2018</risdate><volume>31</volume><issue>8</issue><spage>572</spage><epage>578</epage><pages>572-578</pages><issn>0895-3988</issn><eissn>2214-0190</eissn><abstract>To validate the performance of Mycob. T Stainer and Scanner (MTSS) for detecting acid-fast bacilli (AFB).
A total of 3,816 sputum samples from 1,515 tuberculosis (TB) suspects were tested at the Anhui Provincial Chest Hospital and the Linyi People's Hospital from April-August, 2016. Each specimen was placed on two smear slides. One slide was stained by the ziehl-neelsen (ZN) method to be read by conventional microscopy (CM). The other slide was stained and scanned by MTSS. All specimens were decontaminated with 4% NaOH, and then inoculated into solid culture. The performance of MTSS was assessed.
MTSS produced higher average positivity rate (27.96%) as compared with the CM (26.83%). The overall sensitivity and specificity of MTSS were 78.9% and 93.9%, respectively. The sensitivity and specificity of CM was 77.4% and 95.0%, respectively.
MTSS exhibited a favorable performance in the detection of AFB. It may be an alternative to CM for screening TB.</abstract><cop>China</cop><pub>Elsevier B.V</pub><pmid>30231961</pmid><doi>10.3967/bes2018.078</doi><tpages>7</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Automatic China Hospitals Mycobacterium tuberculosis Scanner Sensitivity and Specificity Sputum - microbiology Stainer Staining and Labeling - methods Tuberculosis Tuberculosis, Pulmonary - diagnosis |
| title | Evaluation of the New Automatic Mycob.T Stainer and Scanner for Detecting Acid-fast Bacilli in China |
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