Overexpression of YOD1 Promotes the Migration of Human Oral Keratinocytes by Enhancing TGF-β3 Signaling
To investigate the effects of YOD1 overexpression on the proliferation and migration of human oral keratinocytes (HOKs), and to clarify whether the mechanisms involve transforming growth factor-β (TGF-β) signaling. HOKs were transfected with the plasmid pEGFP-N3-YOD1 containing YOD1. The mRNA levels...
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Veröffentlicht in: | Biomedical and environmental sciences 2018-07, Vol.31 (7), p.499-506 |
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creator | JU, Qiang LI, Meng Xue CHEN, Gang WANG, Heng Xue SHI, Qiao Mei GE, Xing DING, Zhen WANG, Qi XU, Li Chun |
description | To investigate the effects of YOD1 overexpression on the proliferation and migration of human oral keratinocytes (HOKs), and to clarify whether the mechanisms involve transforming growth factor-β (TGF-β) signaling.
HOKs were transfected with the plasmid pEGFP-N3-YOD1 containing YOD1. The mRNA levels of YOD1 and TGF-β were determined by qPCR. The protein expressions of YOD1, TGF-β, Smad2/3, Smad4, and phospho-Smad2/3 were determined by western blotting. Cell proliferation and migration were evaluated by Cell Counting Kit-8 assay and wound healing assay, respectively.
The mRNA and protein levels of YOD1 were higher in HOKs transfected with YOD1. YOD1 overexpression significantly enhanced the migration of HOKs. The mRNA and protein levels of TGF-β3 were increased by YOD1 overexpression. HOKs transfected with YOD1 exhibited increased phospho-Smad2/3 levels.
YOD1 overexpression enhances cell migration by promoting TGF-β3 signaling which may play an important role in lip and palate formation. YOD1 mutation may contribute to aberrant TGF-β3 signaling associated with decreased cell migration resulting in NSCLP. |
doi_str_mv | 10.3967/bes2018.067 |
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HOKs were transfected with the plasmid pEGFP-N3-YOD1 containing YOD1. The mRNA levels of YOD1 and TGF-β were determined by qPCR. The protein expressions of YOD1, TGF-β, Smad2/3, Smad4, and phospho-Smad2/3 were determined by western blotting. Cell proliferation and migration were evaluated by Cell Counting Kit-8 assay and wound healing assay, respectively.
The mRNA and protein levels of YOD1 were higher in HOKs transfected with YOD1. YOD1 overexpression significantly enhanced the migration of HOKs. The mRNA and protein levels of TGF-β3 were increased by YOD1 overexpression. HOKs transfected with YOD1 exhibited increased phospho-Smad2/3 levels.
YOD1 overexpression enhances cell migration by promoting TGF-β3 signaling which may play an important role in lip and palate formation. YOD1 mutation may contribute to aberrant TGF-β3 signaling associated with decreased cell migration resulting in NSCLP.</description><identifier>ISSN: 0895-3988</identifier><identifier>EISSN: 2214-0190</identifier><identifier>DOI: 10.3967/bes2018.067</identifier><identifier>PMID: 30145984</identifier><language>eng</language><publisher>China: Elsevier B.V</publisher><subject>Cell migration ; Cell Movement - physiology ; Cell Proliferation ; Cells, Cultured ; De-ubiquitinating enzyme YOD1 ; Endopeptidases - genetics ; Endopeptidases - metabolism ; Humans ; Keratinocytes - physiology ; Non-syndromic cleft lip and palate ; Signal Transduction - physiology ; Smad Proteins - genetics ; Smad Proteins - metabolism ; Smads ; Thiolester Hydrolases - genetics ; Thiolester Hydrolases - metabolism ; Transforming Growth Factor beta3 - genetics ; Transforming Growth Factor beta3 - metabolism ; Transforming growth factor-β</subject><ispartof>Biomedical and environmental sciences, 2018-07, Vol.31 (7), p.499-506</ispartof><rights>2018 The Editorial Board of Biomedical and Environmental Sciences</rights><rights>Copyright © 2018 The Editorial Board of Biomedical and Environmental Sciences. Published by China CDC. All rights reserved.</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/bes/bes.jpg</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0895398818300886$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30145984$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>JU, Qiang</creatorcontrib><creatorcontrib>LI, Meng Xue</creatorcontrib><creatorcontrib>CHEN, Gang</creatorcontrib><creatorcontrib>WANG, Heng Xue</creatorcontrib><creatorcontrib>SHI, Qiao Mei</creatorcontrib><creatorcontrib>GE, Xing</creatorcontrib><creatorcontrib>DING, Zhen</creatorcontrib><creatorcontrib>WANG, Qi</creatorcontrib><creatorcontrib>XU, Li Chun</creatorcontrib><title>Overexpression of YOD1 Promotes the Migration of Human Oral Keratinocytes by Enhancing TGF-β3 Signaling</title><title>Biomedical and environmental sciences</title><addtitle>Biomed Environ Sci</addtitle><description>To investigate the effects of YOD1 overexpression on the proliferation and migration of human oral keratinocytes (HOKs), and to clarify whether the mechanisms involve transforming growth factor-β (TGF-β) signaling.
HOKs were transfected with the plasmid pEGFP-N3-YOD1 containing YOD1. The mRNA levels of YOD1 and TGF-β were determined by qPCR. The protein expressions of YOD1, TGF-β, Smad2/3, Smad4, and phospho-Smad2/3 were determined by western blotting. Cell proliferation and migration were evaluated by Cell Counting Kit-8 assay and wound healing assay, respectively.
The mRNA and protein levels of YOD1 were higher in HOKs transfected with YOD1. YOD1 overexpression significantly enhanced the migration of HOKs. The mRNA and protein levels of TGF-β3 were increased by YOD1 overexpression. HOKs transfected with YOD1 exhibited increased phospho-Smad2/3 levels.
YOD1 overexpression enhances cell migration by promoting TGF-β3 signaling which may play an important role in lip and palate formation. YOD1 mutation may contribute to aberrant TGF-β3 signaling associated with decreased cell migration resulting in NSCLP.</description><subject>Cell migration</subject><subject>Cell Movement - physiology</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>De-ubiquitinating enzyme YOD1</subject><subject>Endopeptidases - genetics</subject><subject>Endopeptidases - metabolism</subject><subject>Humans</subject><subject>Keratinocytes - physiology</subject><subject>Non-syndromic cleft lip and palate</subject><subject>Signal Transduction - physiology</subject><subject>Smad Proteins - genetics</subject><subject>Smad Proteins - metabolism</subject><subject>Smads</subject><subject>Thiolester Hydrolases - genetics</subject><subject>Thiolester Hydrolases - metabolism</subject><subject>Transforming Growth Factor beta3 - genetics</subject><subject>Transforming Growth Factor beta3 - metabolism</subject><subject>Transforming growth factor-β</subject><issn>0895-3988</issn><issn>2214-0190</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kc1uFDEQhC0EIkvgxB35wIHLhPbP7NhHlF9E0CIRDpws29PedTTjWeyZwL4WD5Jnyqx2c2qp-lOrqouQ9wzOhF42nx0WDkydwbJ5QRacM1kB0_CSLEDpuhJaqRPyppR7AMm0VK_JiQAma63kgmxWD5jx3zZjKXFIdAj09-qC0R956IcRCx03SL_HdbbjcX0z9TbRVbYd_YZ7OQ1-tyfdjl6mjU0-pjW9u76qHv8L-jOuk-1m5S15FWxX8N1xnpJfV5d35zfV7er66_mX2wrnELwKHhlY5kDzRvPWBnSuroME2bDAtHCu5SFg2-jgAZ1SqJjwmjWMu0a5pTglHw93_9oUbFqb-2HKs4Vijn-CBoDP2KcDts3DnwnLaPpYPHadTThMxXDQUrJ6KfbohyM6uR5bs82xt3lnnp84A_UBwDnXQ8Rsio-YPLYxox9NO0TDwOzLejZh5rLEE7MUhS4</recordid><startdate>20180701</startdate><enddate>20180701</enddate><creator>JU, Qiang</creator><creator>LI, Meng Xue</creator><creator>CHEN, Gang</creator><creator>WANG, Heng Xue</creator><creator>SHI, Qiao Mei</creator><creator>GE, Xing</creator><creator>DING, Zhen</creator><creator>WANG, Qi</creator><creator>XU, Li Chun</creator><general>Elsevier B.V</general><general>School of Public Health, Xuzhou Medical University, Xuzhou 221000, Jiangsu, China</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20180701</creationdate><title>Overexpression of YOD1 Promotes the Migration of Human Oral Keratinocytes by Enhancing TGF-β3 Signaling</title><author>JU, Qiang ; LI, Meng Xue ; CHEN, Gang ; WANG, Heng Xue ; SHI, Qiao Mei ; GE, Xing ; DING, Zhen ; WANG, Qi ; XU, Li Chun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e2012-fce10a1b092792dafebb55f40471f193bbd2ffed79fc0eb88e813c91712b78b63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Cell migration</topic><topic>Cell Movement - physiology</topic><topic>Cell Proliferation</topic><topic>Cells, Cultured</topic><topic>De-ubiquitinating enzyme YOD1</topic><topic>Endopeptidases - genetics</topic><topic>Endopeptidases - metabolism</topic><topic>Humans</topic><topic>Keratinocytes - physiology</topic><topic>Non-syndromic cleft lip and palate</topic><topic>Signal Transduction - physiology</topic><topic>Smad Proteins - genetics</topic><topic>Smad Proteins - metabolism</topic><topic>Smads</topic><topic>Thiolester Hydrolases - genetics</topic><topic>Thiolester Hydrolases - metabolism</topic><topic>Transforming Growth Factor beta3 - genetics</topic><topic>Transforming Growth Factor beta3 - metabolism</topic><topic>Transforming growth factor-β</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>JU, Qiang</creatorcontrib><creatorcontrib>LI, Meng Xue</creatorcontrib><creatorcontrib>CHEN, Gang</creatorcontrib><creatorcontrib>WANG, Heng Xue</creatorcontrib><creatorcontrib>SHI, Qiao Mei</creatorcontrib><creatorcontrib>GE, Xing</creatorcontrib><creatorcontrib>DING, Zhen</creatorcontrib><creatorcontrib>WANG, Qi</creatorcontrib><creatorcontrib>XU, Li Chun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Biomedical and environmental sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>JU, Qiang</au><au>LI, Meng Xue</au><au>CHEN, Gang</au><au>WANG, Heng Xue</au><au>SHI, Qiao Mei</au><au>GE, Xing</au><au>DING, Zhen</au><au>WANG, Qi</au><au>XU, Li Chun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Overexpression of YOD1 Promotes the Migration of Human Oral Keratinocytes by Enhancing TGF-β3 Signaling</atitle><jtitle>Biomedical and environmental sciences</jtitle><addtitle>Biomed Environ Sci</addtitle><date>2018-07-01</date><risdate>2018</risdate><volume>31</volume><issue>7</issue><spage>499</spage><epage>506</epage><pages>499-506</pages><issn>0895-3988</issn><eissn>2214-0190</eissn><abstract>To investigate the effects of YOD1 overexpression on the proliferation and migration of human oral keratinocytes (HOKs), and to clarify whether the mechanisms involve transforming growth factor-β (TGF-β) signaling.
HOKs were transfected with the plasmid pEGFP-N3-YOD1 containing YOD1. The mRNA levels of YOD1 and TGF-β were determined by qPCR. The protein expressions of YOD1, TGF-β, Smad2/3, Smad4, and phospho-Smad2/3 were determined by western blotting. Cell proliferation and migration were evaluated by Cell Counting Kit-8 assay and wound healing assay, respectively.
The mRNA and protein levels of YOD1 were higher in HOKs transfected with YOD1. YOD1 overexpression significantly enhanced the migration of HOKs. The mRNA and protein levels of TGF-β3 were increased by YOD1 overexpression. HOKs transfected with YOD1 exhibited increased phospho-Smad2/3 levels.
YOD1 overexpression enhances cell migration by promoting TGF-β3 signaling which may play an important role in lip and palate formation. YOD1 mutation may contribute to aberrant TGF-β3 signaling associated with decreased cell migration resulting in NSCLP.</abstract><cop>China</cop><pub>Elsevier B.V</pub><pmid>30145984</pmid><doi>10.3967/bes2018.067</doi><tpages>8</tpages></addata></record> |
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subjects | Cell migration Cell Movement - physiology Cell Proliferation Cells, Cultured De-ubiquitinating enzyme YOD1 Endopeptidases - genetics Endopeptidases - metabolism Humans Keratinocytes - physiology Non-syndromic cleft lip and palate Signal Transduction - physiology Smad Proteins - genetics Smad Proteins - metabolism Smads Thiolester Hydrolases - genetics Thiolester Hydrolases - metabolism Transforming Growth Factor beta3 - genetics Transforming Growth Factor beta3 - metabolism Transforming growth factor-β |
title | Overexpression of YOD1 Promotes the Migration of Human Oral Keratinocytes by Enhancing TGF-β3 Signaling |
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