Overexpression of YOD1 Promotes the Migration of Human Oral Keratinocytes by Enhancing TGF-β3 Signaling

To investigate the effects of YOD1 overexpression on the proliferation and migration of human oral keratinocytes (HOKs), and to clarify whether the mechanisms involve transforming growth factor-β (TGF-β) signaling. HOKs were transfected with the plasmid pEGFP-N3-YOD1 containing YOD1. The mRNA levels...

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Veröffentlicht in:Biomedical and environmental sciences 2018-07, Vol.31 (7), p.499-506
Hauptverfasser: JU, Qiang, LI, Meng Xue, CHEN, Gang, WANG, Heng Xue, SHI, Qiao Mei, GE, Xing, DING, Zhen, WANG, Qi, XU, Li Chun
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container_end_page 506
container_issue 7
container_start_page 499
container_title Biomedical and environmental sciences
container_volume 31
creator JU, Qiang
LI, Meng Xue
CHEN, Gang
WANG, Heng Xue
SHI, Qiao Mei
GE, Xing
DING, Zhen
WANG, Qi
XU, Li Chun
description To investigate the effects of YOD1 overexpression on the proliferation and migration of human oral keratinocytes (HOKs), and to clarify whether the mechanisms involve transforming growth factor-β (TGF-β) signaling. HOKs were transfected with the plasmid pEGFP-N3-YOD1 containing YOD1. The mRNA levels of YOD1 and TGF-β were determined by qPCR. The protein expressions of YOD1, TGF-β, Smad2/3, Smad4, and phospho-Smad2/3 were determined by western blotting. Cell proliferation and migration were evaluated by Cell Counting Kit-8 assay and wound healing assay, respectively. The mRNA and protein levels of YOD1 were higher in HOKs transfected with YOD1. YOD1 overexpression significantly enhanced the migration of HOKs. The mRNA and protein levels of TGF-β3 were increased by YOD1 overexpression. HOKs transfected with YOD1 exhibited increased phospho-Smad2/3 levels. YOD1 overexpression enhances cell migration by promoting TGF-β3 signaling which may play an important role in lip and palate formation. YOD1 mutation may contribute to aberrant TGF-β3 signaling associated with decreased cell migration resulting in NSCLP.
doi_str_mv 10.3967/bes2018.067
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HOKs were transfected with the plasmid pEGFP-N3-YOD1 containing YOD1. The mRNA levels of YOD1 and TGF-β were determined by qPCR. The protein expressions of YOD1, TGF-β, Smad2/3, Smad4, and phospho-Smad2/3 were determined by western blotting. Cell proliferation and migration were evaluated by Cell Counting Kit-8 assay and wound healing assay, respectively. The mRNA and protein levels of YOD1 were higher in HOKs transfected with YOD1. YOD1 overexpression significantly enhanced the migration of HOKs. The mRNA and protein levels of TGF-β3 were increased by YOD1 overexpression. HOKs transfected with YOD1 exhibited increased phospho-Smad2/3 levels. YOD1 overexpression enhances cell migration by promoting TGF-β3 signaling which may play an important role in lip and palate formation. YOD1 mutation may contribute to aberrant TGF-β3 signaling associated with decreased cell migration resulting in NSCLP.</description><identifier>ISSN: 0895-3988</identifier><identifier>EISSN: 2214-0190</identifier><identifier>DOI: 10.3967/bes2018.067</identifier><identifier>PMID: 30145984</identifier><language>eng</language><publisher>China: Elsevier B.V</publisher><subject>Cell migration ; Cell Movement - physiology ; Cell Proliferation ; Cells, Cultured ; De-ubiquitinating enzyme YOD1 ; Endopeptidases - genetics ; Endopeptidases - metabolism ; Humans ; Keratinocytes - physiology ; Non-syndromic cleft lip and palate ; Signal Transduction - physiology ; Smad Proteins - genetics ; Smad Proteins - metabolism ; Smads ; Thiolester Hydrolases - genetics ; Thiolester Hydrolases - metabolism ; Transforming Growth Factor beta3 - genetics ; Transforming Growth Factor beta3 - metabolism ; Transforming growth factor-β</subject><ispartof>Biomedical and environmental sciences, 2018-07, Vol.31 (7), p.499-506</ispartof><rights>2018 The Editorial Board of Biomedical and Environmental Sciences</rights><rights>Copyright © 2018 The Editorial Board of Biomedical and Environmental Sciences. Published by China CDC. All rights reserved.</rights><rights>Copyright © Wanfang Data Co. Ltd. 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YOD1 mutation may contribute to aberrant TGF-β3 signaling associated with decreased cell migration resulting in NSCLP.</description><subject>Cell migration</subject><subject>Cell Movement - physiology</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>De-ubiquitinating enzyme YOD1</subject><subject>Endopeptidases - genetics</subject><subject>Endopeptidases - metabolism</subject><subject>Humans</subject><subject>Keratinocytes - physiology</subject><subject>Non-syndromic cleft lip and palate</subject><subject>Signal Transduction - physiology</subject><subject>Smad Proteins - genetics</subject><subject>Smad Proteins - metabolism</subject><subject>Smads</subject><subject>Thiolester Hydrolases - genetics</subject><subject>Thiolester Hydrolases - metabolism</subject><subject>Transforming Growth Factor beta3 - genetics</subject><subject>Transforming Growth Factor beta3 - metabolism</subject><subject>Transforming growth factor-β</subject><issn>0895-3988</issn><issn>2214-0190</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kc1uFDEQhC0EIkvgxB35wIHLhPbP7NhHlF9E0CIRDpws29PedTTjWeyZwL4WD5Jnyqx2c2qp-lOrqouQ9wzOhF42nx0WDkydwbJ5QRacM1kB0_CSLEDpuhJaqRPyppR7AMm0VK_JiQAma63kgmxWD5jx3zZjKXFIdAj09-qC0R956IcRCx03SL_HdbbjcX0z9TbRVbYd_YZ7OQ1-tyfdjl6mjU0-pjW9u76qHv8L-jOuk-1m5S15FWxX8N1xnpJfV5d35zfV7er66_mX2wrnELwKHhlY5kDzRvPWBnSuroME2bDAtHCu5SFg2-jgAZ1SqJjwmjWMu0a5pTglHw93_9oUbFqb-2HKs4Vijn-CBoDP2KcDts3DnwnLaPpYPHadTThMxXDQUrJ6KfbohyM6uR5bs82xt3lnnp84A_UBwDnXQ8Rsio-YPLYxox9NO0TDwOzLejZh5rLEE7MUhS4</recordid><startdate>20180701</startdate><enddate>20180701</enddate><creator>JU, Qiang</creator><creator>LI, Meng Xue</creator><creator>CHEN, Gang</creator><creator>WANG, Heng Xue</creator><creator>SHI, Qiao Mei</creator><creator>GE, Xing</creator><creator>DING, Zhen</creator><creator>WANG, Qi</creator><creator>XU, Li Chun</creator><general>Elsevier B.V</general><general>School of Public Health, Xuzhou Medical University, Xuzhou 221000, Jiangsu, China</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20180701</creationdate><title>Overexpression of YOD1 Promotes the Migration of Human Oral Keratinocytes by Enhancing TGF-β3 Signaling</title><author>JU, Qiang ; 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HOKs were transfected with the plasmid pEGFP-N3-YOD1 containing YOD1. The mRNA levels of YOD1 and TGF-β were determined by qPCR. The protein expressions of YOD1, TGF-β, Smad2/3, Smad4, and phospho-Smad2/3 were determined by western blotting. Cell proliferation and migration were evaluated by Cell Counting Kit-8 assay and wound healing assay, respectively. The mRNA and protein levels of YOD1 were higher in HOKs transfected with YOD1. YOD1 overexpression significantly enhanced the migration of HOKs. The mRNA and protein levels of TGF-β3 were increased by YOD1 overexpression. HOKs transfected with YOD1 exhibited increased phospho-Smad2/3 levels. YOD1 overexpression enhances cell migration by promoting TGF-β3 signaling which may play an important role in lip and palate formation. YOD1 mutation may contribute to aberrant TGF-β3 signaling associated with decreased cell migration resulting in NSCLP.</abstract><cop>China</cop><pub>Elsevier B.V</pub><pmid>30145984</pmid><doi>10.3967/bes2018.067</doi><tpages>8</tpages></addata></record>
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subjects Cell migration
Cell Movement - physiology
Cell Proliferation
Cells, Cultured
De-ubiquitinating enzyme YOD1
Endopeptidases - genetics
Endopeptidases - metabolism
Humans
Keratinocytes - physiology
Non-syndromic cleft lip and palate
Signal Transduction - physiology
Smad Proteins - genetics
Smad Proteins - metabolism
Smads
Thiolester Hydrolases - genetics
Thiolester Hydrolases - metabolism
Transforming Growth Factor beta3 - genetics
Transforming Growth Factor beta3 - metabolism
Transforming growth factor-β
title Overexpression of YOD1 Promotes the Migration of Human Oral Keratinocytes by Enhancing TGF-β3 Signaling
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