The Identification of the Cryptosporidium ubiquitum in Pre-weaned Ovines from Aba Tibetan and Qiang Autonomous Prefecture in China
Objective Cryptosporidium spp. are prevalent globally and sheep are an important zoonotic reservoir. Little data regarding the rates of Cryptosporidium infections in ovines in China are available. This study assessed the prevalence of Cryptosporidium spp. in pre-weaned ovines from Aba Tibetan and Qi...
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| Veröffentlicht in: | Biomedical and environmental sciences 2011-06, Vol.24 (3), p.315-320 |
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| creator | SHEN, YuJuan YIN, JianHai YUAN, ZhongYing LU, WeiYuan XU, YuXin XIAO, LiHua CAO, JianPing |
| description | Objective Cryptosporidium spp. are prevalent globally and sheep are an important zoonotic reservoir. Little data regarding the rates of Cryptosporidium infections in ovines in China are available. This study assessed the prevalence of Cryptosporidium spp. in pre-weaned ovines from Aba Tibetan and Qiang Autonomous Prefecture in the Sichuan province of China. Methods A total of 213 fecal samples were collected from pre-weaned ovines and were examined microscopically （following modified acid fast staining）. In addition, 18S rRNA genetic sequences were amplified from fecal samples by nested PCR and phylogenetically analyzed. Results The prevalence of Cryptosporidium in the collected samples was at 14.6% （31/213） and four isolates identified by PCR belonged to the Cryptosporidium cervine genotype （Cryptosporidium ubiquiturn） demonstrating that this species was the primary sheep species found in sheep in China. Conclusion The present study suggested that the high incidence of Cryptosporidium in sheep poses a significant public health threat and that surveillance practices must be established to prevent zoonotic disease of humans. |
| doi_str_mv | 10.3967/0895-3988.2011.03.016 |
| format | Article |
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Little data regarding the rates of Cryptosporidium infections in ovines in China are available. This study assessed the prevalence of Cryptosporidium spp. in pre-weaned ovines from Aba Tibetan and Qiang Autonomous Prefecture in the Sichuan province of China. Methods A total of 213 fecal samples were collected from pre-weaned ovines and were examined microscopically （following modified acid fast staining）. In addition, 18S rRNA genetic sequences were amplified from fecal samples by nested PCR and phylogenetically analyzed. Results The prevalence of Cryptosporidium in the collected samples was at 14.6% （31/213） and four isolates identified by PCR belonged to the Cryptosporidium cervine genotype （Cryptosporidium ubiquiturn） demonstrating that this species was the primary sheep species found in sheep in China. Conclusion The present study suggested that the high incidence of Cryptosporidium in sheep poses a significant public health threat and that surveillance practices must be established to prevent zoonotic disease of humans.</description><identifier>ISSN: 0895-3988</identifier><identifier>EISSN: 2214-0190</identifier><identifier>DOI: 10.3967/0895-3988.2011.03.016</identifier><identifier>PMID: 21784319</identifier><language>eng</language><publisher>China: Elsevier B.V</publisher><subject>Aba ; Animals ; China ; Cryptosporidium - genetics ; Cryptosporidium - isolation & purification ; Cryptosporidium cervine genotype ; Cryptosporidium ubiquitum ; Feces - parasitology ; Oocysts - microbiology ; Ovines ; PCR鉴定 ; Polymerase Chain Reaction ; rRNA基因序列 ; Sheep ; Weaning ; 中国 ; 人畜共患病 ; 断奶前 ; 系统发育分析 ; 阿坝藏族羌族自治州 ; 隐孢子虫感染</subject><ispartof>Biomedical and environmental sciences, 2011-06, Vol.24 (3), p.315-320</ispartof><rights>2011 The Editorial Board of Biomedical and Environmental Sciences</rights><rights>Copyright © 2011 The Editorial Board of Biomedical and Environmental Sciences. Published by Elsevier B.V. All rights reserved.</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c397t-f6e1ab6b51990100868864ea9b220ac74c60ce57fa179fa3eef82904c71c36573</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/84046X/84046X.jpg</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.3967/0895-3988.2011.03.016$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21784319$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SHEN, YuJuan</creatorcontrib><creatorcontrib>YIN, JianHai</creatorcontrib><creatorcontrib>YUAN, ZhongYing</creatorcontrib><creatorcontrib>LU, WeiYuan</creatorcontrib><creatorcontrib>XU, YuXin</creatorcontrib><creatorcontrib>XIAO, LiHua</creatorcontrib><creatorcontrib>CAO, JianPing</creatorcontrib><title>The Identification of the Cryptosporidium ubiquitum in Pre-weaned Ovines from Aba Tibetan and Qiang Autonomous Prefecture in China</title><title>Biomedical and environmental sciences</title><addtitle>Biomedical and Environmental Sciences</addtitle><description>Objective Cryptosporidium spp. are prevalent globally and sheep are an important zoonotic reservoir. Little data regarding the rates of Cryptosporidium infections in ovines in China are available. This study assessed the prevalence of Cryptosporidium spp. in pre-weaned ovines from Aba Tibetan and Qiang Autonomous Prefecture in the Sichuan province of China. Methods A total of 213 fecal samples were collected from pre-weaned ovines and were examined microscopically （following modified acid fast staining）. In addition, 18S rRNA genetic sequences were amplified from fecal samples by nested PCR and phylogenetically analyzed. Results The prevalence of Cryptosporidium in the collected samples was at 14.6% （31/213） and four isolates identified by PCR belonged to the Cryptosporidium cervine genotype （Cryptosporidium ubiquiturn） demonstrating that this species was the primary sheep species found in sheep in China. Conclusion The present study suggested that the high incidence of Cryptosporidium in sheep poses a significant public health threat and that surveillance practices must be established to prevent zoonotic disease of humans.</description><subject>Aba</subject><subject>Animals</subject><subject>China</subject><subject>Cryptosporidium - genetics</subject><subject>Cryptosporidium - isolation & purification</subject><subject>Cryptosporidium cervine genotype</subject><subject>Cryptosporidium ubiquitum</subject><subject>Feces - parasitology</subject><subject>Oocysts - microbiology</subject><subject>Ovines</subject><subject>PCR鉴定</subject><subject>Polymerase Chain Reaction</subject><subject>rRNA基因序列</subject><subject>Sheep</subject><subject>Weaning</subject><subject>中国</subject><subject>人畜共患病</subject><subject>断奶前</subject><subject>系统发育分析</subject><subject>阿坝藏族羌族自治州</subject><subject>隐孢子虫感染</subject><issn>0895-3988</issn><issn>2214-0190</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kc1u1DAUhSMEokPhEQAjIbHKcB0nsb2qRiN-KlUqiOnacpzrGVcTe2onrbrlyXGUlpUt6_O555xbFO8prJls-VcQsimZFGJdAaVrYGug7YtiVVW0LoFKeFms_jNnxZuUbgFqKmvxujirKBc1o3JV_N0dkFz26EdnndGjC54ES8b8uo2PpzGkU4iud9NAps7dTW7MN-fJr4jlA2qPPbm-dx4TsTEMZNNpsnMdjtoT7Xvy22m_J5tpDD4MYUrzP4tmnCLOKtuD8_pt8crqY8J3T-d5cfP92277s7y6_nG53VyVhkk-lrZFqru2a6iUQAFEK0Rbo5ZdVYE2vDYtGGy41ZRLqxmiFZWE2nBqWNtwdl58XnQftLfZlroNU_R5ouowzSUCyxVm7MuCnWK4mzCNanDJ4PGYw-YESnBJgQpeZ_LDEzl1A_bqFN2g46N6bjcDHxfA6qD0Prqkbv7kSS0A5byhTSYuFgJz8HuHUSXj0BvsXcw1qT44RUHNG1fzNtW8TTWbVcDUYvfTomAOwe_vXM717IMJxuuqBvYPbEalfw</recordid><startdate>20110601</startdate><enddate>20110601</enddate><creator>SHEN, YuJuan</creator><creator>YIN, JianHai</creator><creator>YUAN, ZhongYing</creator><creator>LU, WeiYuan</creator><creator>XU, YuXin</creator><creator>XIAO, LiHua</creator><creator>CAO, JianPing</creator><general>Elsevier B.V</general><general>National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Laboratory of Parasite and Vector Biology, MOH, China, WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, China%Centers for Disease Control and Prevention, Atlanta, Georgia, USA</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20110601</creationdate><title>The Identification of the Cryptosporidium ubiquitum in Pre-weaned Ovines from Aba Tibetan and Qiang Autonomous Prefecture in China</title><author>SHEN, YuJuan ; YIN, JianHai ; YUAN, ZhongYing ; LU, WeiYuan ; XU, YuXin ; XIAO, LiHua ; CAO, JianPing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c397t-f6e1ab6b51990100868864ea9b220ac74c60ce57fa179fa3eef82904c71c36573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Aba</topic><topic>Animals</topic><topic>China</topic><topic>Cryptosporidium - genetics</topic><topic>Cryptosporidium - isolation & purification</topic><topic>Cryptosporidium cervine genotype</topic><topic>Cryptosporidium ubiquitum</topic><topic>Feces - parasitology</topic><topic>Oocysts - microbiology</topic><topic>Ovines</topic><topic>PCR鉴定</topic><topic>Polymerase Chain Reaction</topic><topic>rRNA基因序列</topic><topic>Sheep</topic><topic>Weaning</topic><topic>中国</topic><topic>人畜共患病</topic><topic>断奶前</topic><topic>系统发育分析</topic><topic>阿坝藏族羌族自治州</topic><topic>隐孢子虫感染</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SHEN, YuJuan</creatorcontrib><creatorcontrib>YIN, JianHai</creatorcontrib><creatorcontrib>YUAN, ZhongYing</creatorcontrib><creatorcontrib>LU, WeiYuan</creatorcontrib><creatorcontrib>XU, YuXin</creatorcontrib><creatorcontrib>XIAO, LiHua</creatorcontrib><creatorcontrib>CAO, JianPing</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Biomedical and environmental sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SHEN, YuJuan</au><au>YIN, JianHai</au><au>YUAN, ZhongYing</au><au>LU, WeiYuan</au><au>XU, YuXin</au><au>XIAO, LiHua</au><au>CAO, JianPing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Identification of the Cryptosporidium ubiquitum in Pre-weaned Ovines from Aba Tibetan and Qiang Autonomous Prefecture in China</atitle><jtitle>Biomedical and environmental sciences</jtitle><addtitle>Biomedical and Environmental Sciences</addtitle><date>2011-06-01</date><risdate>2011</risdate><volume>24</volume><issue>3</issue><spage>315</spage><epage>320</epage><pages>315-320</pages><issn>0895-3988</issn><eissn>2214-0190</eissn><abstract>Objective Cryptosporidium spp. are prevalent globally and sheep are an important zoonotic reservoir. Little data regarding the rates of Cryptosporidium infections in ovines in China are available. This study assessed the prevalence of Cryptosporidium spp. in pre-weaned ovines from Aba Tibetan and Qiang Autonomous Prefecture in the Sichuan province of China. Methods A total of 213 fecal samples were collected from pre-weaned ovines and were examined microscopically （following modified acid fast staining）. In addition, 18S rRNA genetic sequences were amplified from fecal samples by nested PCR and phylogenetically analyzed. Results The prevalence of Cryptosporidium in the collected samples was at 14.6% （31/213） and four isolates identified by PCR belonged to the Cryptosporidium cervine genotype （Cryptosporidium ubiquiturn） demonstrating that this species was the primary sheep species found in sheep in China. Conclusion The present study suggested that the high incidence of Cryptosporidium in sheep poses a significant public health threat and that surveillance practices must be established to prevent zoonotic disease of humans.</abstract><cop>China</cop><pub>Elsevier B.V</pub><pmid>21784319</pmid><doi>10.3967/0895-3988.2011.03.016</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biomedical and environmental sciences, 2011-06, Vol.24 (3), p.315-320 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Aba Animals China Cryptosporidium - genetics Cryptosporidium - isolation & purification Cryptosporidium cervine genotype Cryptosporidium ubiquitum Feces - parasitology Oocysts - microbiology Ovines PCR鉴定 Polymerase Chain Reaction rRNA基因序列 Sheep Weaning 中国 人畜共患病 断奶前 系统发育分析 阿坝藏族羌族自治州 隐孢子虫感染 |
| title | The Identification of the Cryptosporidium ubiquitum in Pre-weaned Ovines from Aba Tibetan and Qiang Autonomous Prefecture in China |
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