Large-scale Purification and Acute Toxicity of Hygromycin B Phosphotransferase

Objective To provide the acute toxicity data of hygromycin B phosphotransferase （HPT） using recombinant protein purified from E. coli. Methods Recombinant HPT protein was expressed and purified from E. coli. To exclude the potential adverse effect of bacteria protein in recombinant HPT protein, bact...

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Veröffentlicht in:	Biomedical and environmental sciences 2009-02, Vol.22 (1), p.22-27
Hauptverfasser:	ZHUO, Qin, PIAO, Jian-Hua, TIAN, Yuan, XU, Jie, YANG, Xiao-Guang
Format:	Artikel
Sprache:	eng
Schlagworte:	Acute toxicity Animals Bacterial Proteins - isolation & purification Bacterial Proteins - toxicity Escherichia coli - genetics Escherichia coli - metabolism Female Hygromycin B phosphotransferase Male Mice Mice, Inbred Strains Phosphotransferases (Alcohol Group Acceptor) - isolation & purification Phosphotransferases (Alcohol Group Acceptor) - toxicity Recombinant Proteins - isolation & purification Recombinant Proteins - toxicity Safety assessment Selectable marker Toxicity Tests, Acute 急性毒性潮霉素生物医学磷酸转移酶重组细胞
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