Effect of lidamycin on telomerase activity in human hepatoma BEL-7402 cells
To investigate the effect of lidamycin (LDM) on telomerase activity in human hepatoma BEL-7402 cells under the condition of LDM inducing mitotic cell death and senescence. Chromatin condensation was detected by co-staining with Hoechst 33342 and PI. Cell multinucleation was observed by Giemsa staini...
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| Veröffentlicht in: | Biomedical and environmental sciences 2007-06, Vol.20 (3), p.189-197 |
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| creator | Gao, Rui-Juan Liang, Yue-Xin Li, Dian-Dong Zhang, Hong-Yin Zhen, Yong-Su |
| description | To investigate the effect of lidamycin (LDM) on telomerase activity in human hepatoma BEL-7402 cells under the condition of LDM inducing mitotic cell death and senescence.
Chromatin condensation was detected by co-staining with Hoechst 33342 and PI. Cell multinucleation was observed by Giemsa staining and genomic DNA was separated by agarose gel electrophoresis. Fluorescent intensity of Rho123 was determined for mitochondrial membrane potential. MTT assay and SA-beta-gal staining were employed to analyze the senescence-like phenotype. The expression of proteins was analyzed by Western blot. Telomerase activity was assayed by telomerase PCR-ELISA.
Mitotic cell death occurred in LDM-treated cells characterized by unique and atypical chromatin condensation, multinucleation and increased mitochondrial membrane potential. However, no apoptotic bodies or DNA ladders were found. In addition, apoptosis-related proteins remained nearly unaltered. Senescence-like phenotype was identified by increased and elongated size of cells, growth retardation, enhanced SA-beta-gal activity and the changes of senescence-related protein expression. Telomerase activity markedly decreased (P |
| format | Article |
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Chromatin condensation was detected by co-staining with Hoechst 33342 and PI. Cell multinucleation was observed by Giemsa staining and genomic DNA was separated by agarose gel electrophoresis. Fluorescent intensity of Rho123 was determined for mitochondrial membrane potential. MTT assay and SA-beta-gal staining were employed to analyze the senescence-like phenotype. The expression of proteins was analyzed by Western blot. Telomerase activity was assayed by telomerase PCR-ELISA.
Mitotic cell death occurred in LDM-treated cells characterized by unique and atypical chromatin condensation, multinucleation and increased mitochondrial membrane potential. However, no apoptotic bodies or DNA ladders were found. In addition, apoptosis-related proteins remained nearly unaltered. Senescence-like phenotype was identified by increased and elongated size of cells, growth retardation, enhanced SA-beta-gal activity and the changes of senescence-related protein expression. Telomerase activity markedly decreased (P<0.01) in LDM-treated hepatoma BEL-7402 cells.
Mitotic cell death and senescence could be triggered simultaneously or sequentially after exposure of hepatoma BEL-7402 cells to LDM. The decrease in telomerase activity may play a key role in the defective mitosis and aging morphology. Further investigation of detailed mechanism is needed.</description><identifier>ISSN: 0895-3988</identifier><identifier>PMID: 17672208</identifier><language>eng</language><publisher>China: Institute of Medicinal Biotechnology,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100050,China%Peking University Hospital,Beijing 100871,China</publisher><subject>Aminoglycosides - pharmacology ; Antibiotics, Antineoplastic - pharmacology ; Apoptosis - drug effects ; Azure Stains ; Benzimidazoles ; beta-Galactosidase - metabolism ; Carcinoma, Hepatocellular - enzymology ; Carcinoma, Hepatocellular - pathology ; Cell Nucleus - drug effects ; Cell Nucleus - metabolism ; Cellular Senescence - drug effects ; Chromatin - metabolism ; DNA, Neoplasm - analysis ; Dose-Response Relationship, Drug ; Enediynes - pharmacology ; Genome, Human - genetics ; Humans ; Liver Neoplasms - enzymology ; Liver Neoplasms - pathology ; Membrane Potential, Mitochondrial - drug effects ; Mitosis - drug effects ; Phenotype ; Propidium ; Telomerase - metabolism ; Time Factors</subject><ispartof>Biomedical and environmental sciences, 2007-06, Vol.20 (3), p.189-197</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/bes/bes.jpg</thumbnail><link.rule.ids>314,777,781</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17672208$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gao, Rui-Juan</creatorcontrib><creatorcontrib>Liang, Yue-Xin</creatorcontrib><creatorcontrib>Li, Dian-Dong</creatorcontrib><creatorcontrib>Zhang, Hong-Yin</creatorcontrib><creatorcontrib>Zhen, Yong-Su</creatorcontrib><title>Effect of lidamycin on telomerase activity in human hepatoma BEL-7402 cells</title><title>Biomedical and environmental sciences</title><addtitle>Biomed Environ Sci</addtitle><description>To investigate the effect of lidamycin (LDM) on telomerase activity in human hepatoma BEL-7402 cells under the condition of LDM inducing mitotic cell death and senescence.
Chromatin condensation was detected by co-staining with Hoechst 33342 and PI. Cell multinucleation was observed by Giemsa staining and genomic DNA was separated by agarose gel electrophoresis. Fluorescent intensity of Rho123 was determined for mitochondrial membrane potential. MTT assay and SA-beta-gal staining were employed to analyze the senescence-like phenotype. The expression of proteins was analyzed by Western blot. Telomerase activity was assayed by telomerase PCR-ELISA.
Mitotic cell death occurred in LDM-treated cells characterized by unique and atypical chromatin condensation, multinucleation and increased mitochondrial membrane potential. However, no apoptotic bodies or DNA ladders were found. In addition, apoptosis-related proteins remained nearly unaltered. Senescence-like phenotype was identified by increased and elongated size of cells, growth retardation, enhanced SA-beta-gal activity and the changes of senescence-related protein expression. Telomerase activity markedly decreased (P<0.01) in LDM-treated hepatoma BEL-7402 cells.
Mitotic cell death and senescence could be triggered simultaneously or sequentially after exposure of hepatoma BEL-7402 cells to LDM. The decrease in telomerase activity may play a key role in the defective mitosis and aging morphology. Further investigation of detailed mechanism is needed.</description><subject>Aminoglycosides - pharmacology</subject><subject>Antibiotics, Antineoplastic - pharmacology</subject><subject>Apoptosis - drug effects</subject><subject>Azure Stains</subject><subject>Benzimidazoles</subject><subject>beta-Galactosidase - metabolism</subject><subject>Carcinoma, Hepatocellular - enzymology</subject><subject>Carcinoma, Hepatocellular - pathology</subject><subject>Cell Nucleus - drug effects</subject><subject>Cell Nucleus - metabolism</subject><subject>Cellular Senescence - drug effects</subject><subject>Chromatin - metabolism</subject><subject>DNA, Neoplasm - analysis</subject><subject>Dose-Response Relationship, Drug</subject><subject>Enediynes - pharmacology</subject><subject>Genome, Human - genetics</subject><subject>Humans</subject><subject>Liver Neoplasms - enzymology</subject><subject>Liver Neoplasms - pathology</subject><subject>Membrane Potential, Mitochondrial - drug effects</subject><subject>Mitosis - drug effects</subject><subject>Phenotype</subject><subject>Propidium</subject><subject>Telomerase - metabolism</subject><subject>Time Factors</subject><issn>0895-3988</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kLFOwzAQhj2AaCm8AvKA2CI5dhI7I1SBIiKxwBydnTOkiuMQO6C-PUEty91wn_7v9J-RNVNlnohSqRW5DGHPWJaWmbogq1QWknOm1uSlshZNpN7SvmvBHUw3UD_QiL13OEFACiZ231080OXyOTtYJo4QvQP6UNWJzBinBvs-XJFzC33A69PekPfH6m27S-rXp-ftfZ2MXKiYICtTbQxDLotW6LxEnqbKCAG2MFKC1AjKGgTBTWs56FQXrVUZV4pbU1qxIbfH3B8YLAwfzd7P07AYG42BMyaZYEws2N0RGyf_NWOIjevC36MwoJ9DU6hFm-VyAW9O4Kwdts04dQ6mQ_PfkvgFWcthqg</recordid><startdate>20070601</startdate><enddate>20070601</enddate><creator>Gao, Rui-Juan</creator><creator>Liang, Yue-Xin</creator><creator>Li, Dian-Dong</creator><creator>Zhang, Hong-Yin</creator><creator>Zhen, Yong-Su</creator><general>Institute of Medicinal Biotechnology,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100050,China%Peking University Hospital,Beijing 100871,China</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20070601</creationdate><title>Effect of lidamycin on telomerase activity in human hepatoma BEL-7402 cells</title><author>Gao, Rui-Juan ; Liang, Yue-Xin ; Li, Dian-Dong ; Zhang, Hong-Yin ; Zhen, Yong-Su</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p238t-e091bcc0e276d3b59e2118c33af6c77a7bea8fcea32cdf2ab1b6df842882fc9f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Aminoglycosides - pharmacology</topic><topic>Antibiotics, Antineoplastic - pharmacology</topic><topic>Apoptosis - drug effects</topic><topic>Azure Stains</topic><topic>Benzimidazoles</topic><topic>beta-Galactosidase - metabolism</topic><topic>Carcinoma, Hepatocellular - enzymology</topic><topic>Carcinoma, Hepatocellular - pathology</topic><topic>Cell Nucleus - drug effects</topic><topic>Cell Nucleus - metabolism</topic><topic>Cellular Senescence - drug effects</topic><topic>Chromatin - metabolism</topic><topic>DNA, Neoplasm - analysis</topic><topic>Dose-Response Relationship, Drug</topic><topic>Enediynes - pharmacology</topic><topic>Genome, Human - genetics</topic><topic>Humans</topic><topic>Liver Neoplasms - enzymology</topic><topic>Liver Neoplasms - pathology</topic><topic>Membrane Potential, Mitochondrial - drug effects</topic><topic>Mitosis - drug effects</topic><topic>Phenotype</topic><topic>Propidium</topic><topic>Telomerase - metabolism</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gao, Rui-Juan</creatorcontrib><creatorcontrib>Liang, Yue-Xin</creatorcontrib><creatorcontrib>Li, Dian-Dong</creatorcontrib><creatorcontrib>Zhang, Hong-Yin</creatorcontrib><creatorcontrib>Zhen, Yong-Su</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Biomedical and environmental sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gao, Rui-Juan</au><au>Liang, Yue-Xin</au><au>Li, Dian-Dong</au><au>Zhang, Hong-Yin</au><au>Zhen, Yong-Su</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of lidamycin on telomerase activity in human hepatoma BEL-7402 cells</atitle><jtitle>Biomedical and environmental sciences</jtitle><addtitle>Biomed Environ Sci</addtitle><date>2007-06-01</date><risdate>2007</risdate><volume>20</volume><issue>3</issue><spage>189</spage><epage>197</epage><pages>189-197</pages><issn>0895-3988</issn><abstract>To investigate the effect of lidamycin (LDM) on telomerase activity in human hepatoma BEL-7402 cells under the condition of LDM inducing mitotic cell death and senescence.
Chromatin condensation was detected by co-staining with Hoechst 33342 and PI. Cell multinucleation was observed by Giemsa staining and genomic DNA was separated by agarose gel electrophoresis. Fluorescent intensity of Rho123 was determined for mitochondrial membrane potential. MTT assay and SA-beta-gal staining were employed to analyze the senescence-like phenotype. The expression of proteins was analyzed by Western blot. Telomerase activity was assayed by telomerase PCR-ELISA.
Mitotic cell death occurred in LDM-treated cells characterized by unique and atypical chromatin condensation, multinucleation and increased mitochondrial membrane potential. However, no apoptotic bodies or DNA ladders were found. In addition, apoptosis-related proteins remained nearly unaltered. Senescence-like phenotype was identified by increased and elongated size of cells, growth retardation, enhanced SA-beta-gal activity and the changes of senescence-related protein expression. Telomerase activity markedly decreased (P<0.01) in LDM-treated hepatoma BEL-7402 cells.
Mitotic cell death and senescence could be triggered simultaneously or sequentially after exposure of hepatoma BEL-7402 cells to LDM. The decrease in telomerase activity may play a key role in the defective mitosis and aging morphology. Further investigation of detailed mechanism is needed.</abstract><cop>China</cop><pub>Institute of Medicinal Biotechnology,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100050,China%Peking University Hospital,Beijing 100871,China</pub><pmid>17672208</pmid><tpages>9</tpages></addata></record> |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Aminoglycosides - pharmacology Antibiotics, Antineoplastic - pharmacology Apoptosis - drug effects Azure Stains Benzimidazoles beta-Galactosidase - metabolism Carcinoma, Hepatocellular - enzymology Carcinoma, Hepatocellular - pathology Cell Nucleus - drug effects Cell Nucleus - metabolism Cellular Senescence - drug effects Chromatin - metabolism DNA, Neoplasm - analysis Dose-Response Relationship, Drug Enediynes - pharmacology Genome, Human - genetics Humans Liver Neoplasms - enzymology Liver Neoplasms - pathology Membrane Potential, Mitochondrial - drug effects Mitosis - drug effects Phenotype Propidium Telomerase - metabolism Time Factors |
| title | Effect of lidamycin on telomerase activity in human hepatoma BEL-7402 cells |
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