Cloning and Sequence Analysis of the Full-length cDNA of a Novel yp05 Gene Associated With Citrinin Production in Monascus aurantiacus
Objective To obtain the full-length cDNA of a novel gene （named yp05） associated with citrinin production-related genes in Monascus aurantiacus. Methods Total RNA was extracted from mycelium, 3＇ and 5＇ cDNA end of yp05 gene was amplified using smartTM trace cDNA amplification kit, and the full-lengt...
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| Veröffentlicht in: | Biomedical and environmental sciences 2007-04, Vol.20 (2), p.135-140 |
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| creator | Xiong, Yong-Hua Xu, Yang Lai, Wei-Hua Li, Yan-Pin Wei, Hua |
| description | Objective To obtain the full-length cDNA of a novel gene （named yp05） associated with citrinin production-related genes in Monascus aurantiacus. Methods Total RNA was extracted from mycelium, 3＇ and 5＇ cDNA end of yp05 gene was amplified using smartTM trace cDNA amplification kit, and the full-length cDNA of a novel gene （named yp05） was obtained from the electronic assembly of 3＇-RACE and 5＇- RACE products. Results This yp05 gene was 787 bp including a 597 bp open reading frame （ORF） and encoded a deduced protein with 199 amino acid residues, and the amino acid sequence of this protein was found similar with the sequences of many fungal manganese-superoxide dismutases in the GenBank with the aid of BLASTp. The transcription ofyp05 gene in Monascus strains was analyzed with the aid of Northern blotting. The transcription of yp05 gene was only detected in Monascus strains, provided that citrinin was produced. Conclusion The transcription of yp05 gene belongs to differential expression genes of citfinin yielded from Monascus and has no correlation with the biosynthesis pathway of red pigments. |
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Methods Total RNA was extracted from mycelium, 3＇ and 5＇ cDNA end of yp05 gene was amplified using smartTM trace cDNA amplification kit, and the full-length cDNA of a novel gene （named yp05） was obtained from the electronic assembly of 3＇-RACE and 5＇- RACE products. Results This yp05 gene was 787 bp including a 597 bp open reading frame （ORF） and encoded a deduced protein with 199 amino acid residues, and the amino acid sequence of this protein was found similar with the sequences of many fungal manganese-superoxide dismutases in the GenBank with the aid of BLASTp. The transcription ofyp05 gene in Monascus strains was analyzed with the aid of Northern blotting. The transcription of yp05 gene was only detected in Monascus strains, provided that citrinin was produced. Conclusion The transcription of yp05 gene belongs to differential expression genes of citfinin yielded from Monascus and has no correlation with the biosynthesis pathway of red pigments.</description><identifier>ISSN: 0895-3988</identifier><identifier>EISSN: 2214-0190</identifier><identifier>PMID: 17624188</identifier><language>eng</language><publisher>China: Sino-Germany Joint Research Institute, National Key Laboratory of Food Science (Nanchang University), Ministry of Education, Nanchang 330047, Jiangxi, China</publisher><subject>Amino Acid Sequence ; Base Sequence ; Blotting, Northern ; cDNA ; Citrinin - biosynthesis ; Cloning, Molecular ; DNA, Complementary - chemistry ; Fungal Proteins - chemistry ; Fungal Proteins - genetics ; Gene Library ; Molecular Sequence Data ; Monascus - genetics ; Monascus - metabolism ; Mycelium - genetics ; Mycelium - metabolism ; Pigments, Biological - biosynthesis ; RNA, Messenger - metabolism ; Sequence Alignment ; Sequence Analysis, DNA ; yp05 ; 全基因组 ; 基因克隆 ; 序列分析 ; 橘霉素生产 ; 红曲霉</subject><ispartof>Biomedical and environmental sciences, 2007-04, Vol.20 (2), p.135-140</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/84046X/84046X.jpg</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17624188$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xiong, Yong-Hua</creatorcontrib><creatorcontrib>Xu, Yang</creatorcontrib><creatorcontrib>Lai, Wei-Hua</creatorcontrib><creatorcontrib>Li, Yan-Pin</creatorcontrib><creatorcontrib>Wei, Hua</creatorcontrib><title>Cloning and Sequence Analysis of the Full-length cDNA of a Novel yp05 Gene Associated With Citrinin Production in Monascus aurantiacus</title><title>Biomedical and environmental sciences</title><addtitle>Biomedical and Environmental Sciences</addtitle><description>Objective To obtain the full-length cDNA of a novel gene （named yp05） associated with citrinin production-related genes in Monascus aurantiacus. Methods Total RNA was extracted from mycelium, 3＇ and 5＇ cDNA end of yp05 gene was amplified using smartTM trace cDNA amplification kit, and the full-length cDNA of a novel gene （named yp05） was obtained from the electronic assembly of 3＇-RACE and 5＇- RACE products. Results This yp05 gene was 787 bp including a 597 bp open reading frame （ORF） and encoded a deduced protein with 199 amino acid residues, and the amino acid sequence of this protein was found similar with the sequences of many fungal manganese-superoxide dismutases in the GenBank with the aid of BLASTp. The transcription ofyp05 gene in Monascus strains was analyzed with the aid of Northern blotting. The transcription of yp05 gene was only detected in Monascus strains, provided that citrinin was produced. Conclusion The transcription of yp05 gene belongs to differential expression genes of citfinin yielded from Monascus and has no correlation with the biosynthesis pathway of red pigments.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Blotting, Northern</subject><subject>cDNA</subject><subject>Citrinin - biosynthesis</subject><subject>Cloning, Molecular</subject><subject>DNA, Complementary - chemistry</subject><subject>Fungal Proteins - chemistry</subject><subject>Fungal Proteins - genetics</subject><subject>Gene Library</subject><subject>Molecular Sequence Data</subject><subject>Monascus - genetics</subject><subject>Monascus - metabolism</subject><subject>Mycelium - genetics</subject><subject>Mycelium - metabolism</subject><subject>Pigments, Biological - biosynthesis</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, DNA</subject><subject>yp05</subject><subject>全基因组</subject><subject>基因克隆</subject><subject>序列分析</subject><subject>橘霉素生产</subject><subject>红曲霉</subject><issn>0895-3988</issn><issn>2214-0190</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo10N9r2zAQB3AzNtqs7b8wRBl9M5x-OJIfQ7a2gy4btKOP5iyfE7WKlFh2R_6B_d1VSfei0x0fHej7oZgJwVUJvIaPxQxMXZWyNua0-JzSE4DitTInxSnXc6G4MbPi39LH4MKaYejYPe0nCpbYIqA_JJdY7Nm4IXY9eV96Cutxw-y31eJtjmwVX8izww4qdkMhv0opWocjdezRZbl04-DycvZ7iN1kRxcDy93PGDDZKTGcBgyjw3w_Lz716BNdvNez4s_194flbXn36-bHcnFXWm7AlCjrStlakzIoQYiqM9oCB0Et6qqzvaTW1tQJMTdQK5gbUWGPvMpcG23kWfH1uPcvhh7DunmK05A_m5qWkgDQkI83dnVkuyHmSNLYbF2y5D0GilNqNGgupNIZfnmHU7ulrtkNbovDofkfcAaXR2A3Maz3OemmRfvcO0-NUBKkkly-AnLxgkM</recordid><startdate>200704</startdate><enddate>200704</enddate><creator>Xiong, Yong-Hua</creator><creator>Xu, Yang</creator><creator>Lai, Wei-Hua</creator><creator>Li, Yan-Pin</creator><creator>Wei, Hua</creator><general>Sino-Germany Joint Research Institute, National Key Laboratory of Food Science (Nanchang University), Ministry of Education, Nanchang 330047, Jiangxi, China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W92</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>200704</creationdate><title>Cloning and Sequence Analysis of the Full-length cDNA of a Novel yp05 Gene Associated With Citrinin Production in Monascus aurantiacus</title><author>Xiong, Yong-Hua ; Xu, Yang ; Lai, Wei-Hua ; Li, Yan-Pin ; Wei, Hua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1808-a3954c97e48a30225d87c0102eba75dcf3ebc9ed226809406825afa158a378783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Blotting, Northern</topic><topic>cDNA</topic><topic>Citrinin - biosynthesis</topic><topic>Cloning, Molecular</topic><topic>DNA, Complementary - chemistry</topic><topic>Fungal Proteins - chemistry</topic><topic>Fungal Proteins - genetics</topic><topic>Gene Library</topic><topic>Molecular Sequence Data</topic><topic>Monascus - genetics</topic><topic>Monascus - metabolism</topic><topic>Mycelium - genetics</topic><topic>Mycelium - metabolism</topic><topic>Pigments, Biological - biosynthesis</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Alignment</topic><topic>Sequence Analysis, DNA</topic><topic>yp05</topic><topic>全基因组</topic><topic>基因克隆</topic><topic>序列分析</topic><topic>橘霉素生产</topic><topic>红曲霉</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xiong, Yong-Hua</creatorcontrib><creatorcontrib>Xu, Yang</creatorcontrib><creatorcontrib>Lai, Wei-Hua</creatorcontrib><creatorcontrib>Li, Yan-Pin</creatorcontrib><creatorcontrib>Wei, Hua</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-工程技术</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Biomedical and environmental sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xiong, Yong-Hua</au><au>Xu, Yang</au><au>Lai, Wei-Hua</au><au>Li, Yan-Pin</au><au>Wei, Hua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and Sequence Analysis of the Full-length cDNA of a Novel yp05 Gene Associated With Citrinin Production in Monascus aurantiacus</atitle><jtitle>Biomedical and environmental sciences</jtitle><addtitle>Biomedical and Environmental Sciences</addtitle><date>2007-04</date><risdate>2007</risdate><volume>20</volume><issue>2</issue><spage>135</spage><epage>140</epage><pages>135-140</pages><issn>0895-3988</issn><eissn>2214-0190</eissn><abstract>Objective To obtain the full-length cDNA of a novel gene （named yp05） associated with citrinin production-related genes in Monascus aurantiacus. Methods Total RNA was extracted from mycelium, 3＇ and 5＇ cDNA end of yp05 gene was amplified using smartTM trace cDNA amplification kit, and the full-length cDNA of a novel gene （named yp05） was obtained from the electronic assembly of 3＇-RACE and 5＇- RACE products. Results This yp05 gene was 787 bp including a 597 bp open reading frame （ORF） and encoded a deduced protein with 199 amino acid residues, and the amino acid sequence of this protein was found similar with the sequences of many fungal manganese-superoxide dismutases in the GenBank with the aid of BLASTp. The transcription ofyp05 gene in Monascus strains was analyzed with the aid of Northern blotting. The transcription of yp05 gene was only detected in Monascus strains, provided that citrinin was produced. Conclusion The transcription of yp05 gene belongs to differential expression genes of citfinin yielded from Monascus and has no correlation with the biosynthesis pathway of red pigments.</abstract><cop>China</cop><pub>Sino-Germany Joint Research Institute, National Key Laboratory of Food Science (Nanchang University), Ministry of Education, Nanchang 330047, Jiangxi, China</pub><pmid>17624188</pmid><tpages>6</tpages></addata></record> |
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| ispartof | Biomedical and environmental sciences, 2007-04, Vol.20 (2), p.135-140 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Base Sequence Blotting, Northern cDNA Citrinin - biosynthesis Cloning, Molecular DNA, Complementary - chemistry Fungal Proteins - chemistry Fungal Proteins - genetics Gene Library Molecular Sequence Data Monascus - genetics Monascus - metabolism Mycelium - genetics Mycelium - metabolism Pigments, Biological - biosynthesis RNA, Messenger - metabolism Sequence Alignment Sequence Analysis, DNA yp05 全基因组 基因克隆 序列分析 橘霉素生产 红曲霉 |
| title | Cloning and Sequence Analysis of the Full-length cDNA of a Novel yp05 Gene Associated With Citrinin Production in Monascus aurantiacus |
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