Specific Detection of Toxigenic Vibrio cholerae Based on in situ PCR in Combination With Flow Cytometry

Objective To develop an in situ PCR in combination with flow cytometry （ISPCR-FCM） for monitoring cholera toxin positive Vibrio cholerae. Methods In running this method, 4% paraformaldehyde was used to fix the Vibrio cholerae cells and 1 mg/mL lysozyme for 20 min to permeabilize the cells. Before th...

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Veröffentlicht in:	Biomedical and environmental sciences 2007-02, Vol.20 (1), p.64-69
Hauptverfasser:	Zhu, Li, Cai, Jun-Peng, Chen, Qing, Yu, Shou-Yi
Format:	Artikel
Sprache:	eng
Schlagworte:	Cholera Toxin - genetics Colony Count, Microbial DNA, Bacterial - analysis Flow Cytometry Genes, Bacterial - genetics Microscopy, Fluorescence Polymerase Chain Reaction Reproducibility of Results Vibrio cholerae - genetics Vibrio cholerae - isolation & purification 产毒霍乱弧菌 原位PCR 流动细胞计数 特异性检测 联合检测
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