Micro device for cell culture

A micro device for cell culture is disclosed, which cooperates with a fluid and includes: a top plate having an inlet port; a orifice plate having a plurality of orifices; a culture plate having a plurality of culture wells and a plurality of injection ports; and a bottom plate having at least one collecting well and at least one collecting flow channel, wherein, the culture plate is placed between the orifice plate and the bottom plate. The collecting flow channel connects to all regulating orifices in the culture wells and guides the fluid from the culture wells, then receives the fluid in the collecting well. The fluid flows into the orifice plate from the inlet port of the top plate, and then diversifies into the culture plate, then arrives at each culture well by way of the injection ports, and finally collects in the collecting well of the bottom plate.