Method for cloning and expression of Acc65I restriction endonuclease and Acc65I methylase in

Method for cloning and expression of Acc65I restriction endonuclease and Acc65I methylase in

−02 An isolated DNA is provided which encodes a protein that is capable of binding to 5′GGTACC-3′, the isolated DNA being capable of hybridizing to SEQ ID NO:3 under stringent hybridization conditions. The isolated DNA may be alternatively characterized as coding for a protein having an amino acid s...

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Hauptverfasser: Lunnen, Keith, Greci, John, Wilson, Geoffrey
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creator Lunnen, Keith
Greci, John
Wilson, Geoffrey
description −02 An isolated DNA is provided which encodes a protein that is capable of binding to 5′GGTACC-3′, the isolated DNA being capable of hybridizing to SEQ ID NO:3 under stringent hybridization conditions. The isolated DNA may be alternatively characterized as coding for a protein having an amino acid sequence comprising SEQ ID NO:5 or by an amino acid sequence with an expectation value of less than E=ein a BLAST search using SEQ ID NO:5. Vectors containing the isolated DNA and host cells expressing the vectors as well as a method for making recombinant Acc65I having the above properties are also provided.
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The isolated DNA may be alternatively characterized as coding for a protein having an amino acid sequence comprising SEQ ID NO:5 or by an amino acid sequence with an expectation value of less than E=ein a BLAST search using SEQ ID NO:5. Vectors containing the isolated DNA and host cells expressing the vectors as well as a method for making recombinant Acc65I having the above properties are also provided.</abstract><oa>free_for_read</oa></addata></record>
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title Method for cloning and expression of Acc65I restriction endonuclease and Acc65I methylase in
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