Process for isolating sea cucumber saponin Frondoside A, and immunomodulatory methods of use

Process for isolating sea cucumber saponin Frondoside A, and immunomodulatory methods of use

Cucumaria frondosaThis present invention discloses use of a chloroform/solvent mixture extraction at different solvent-to-feed ratios, followed by evaporation and extraction with ethyl acetate/water; followed by chromatography of the water phase in Teflon or other non-polar resin and Silica gel colu...

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Hauptverfasser: Avilov, Sergey Anatolievuch, Kalinin, Vladimir Ivanovich, Silchenko, Alexandra Sergeevna, Aminin, Dmitry Lvovich, Agafonova, Irina Grigorevna, Stonik, Valentin Aronovich, Collin, Peter D, Woodward, Carl
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creator Avilov, Sergey Anatolievuch
Kalinin, Vladimir Ivanovich
Silchenko, Alexandra Sergeevna
Aminin, Dmitry Lvovich
Agafonova, Irina Grigorevna
Stonik, Valentin Aronovich
Collin, Peter D
Woodward, Carl
description Cucumaria frondosaThis present invention discloses use of a chloroform/solvent mixture extraction at different solvent-to-feed ratios, followed by evaporation and extraction with ethyl acetate/water; followed by chromatography of the water phase in Teflon or other non-polar resin and Silica gel column chromatography for recovering of individual triterpene glycosides (saponins) of high purity from the freeze dried or spray dried cooking water or dried powderized tissues of the industrial processed sea cucumber . A resulting glycoside Frondoside A stimulates lysosomal activity of peritoneal macrophages, phagocytosis and oxidative burst in the macrophages at concentrations significantly less than for acute toxicity, hemolysis and sea urchin embryo toxicity.
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title Process for isolating sea cucumber saponin Frondoside A, and immunomodulatory methods of use
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