Luciferase detection reagent kits and luciferase detection method using the kit

Luciferase detection reagent kits and luciferase detection method using the kit

Coleoptera luciferaseColeoptera luciferaseColeoptera luciferaseThe present invention provides a detection method and a method of manufacturing a detection kit, both characterized by use of an organic sulfur reagent, and which are effective at low concentration of the reagent, are inexpensive, and ha...

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Hauptverfasser: Ryufuku, Masayuki, Tanaka, Hozumi, Suzuki, Chie
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creator Ryufuku, Masayuki
Tanaka, Hozumi
Suzuki, Chie
description Coleoptera luciferaseColeoptera luciferaseColeoptera luciferaseThe present invention provides a detection method and a method of manufacturing a detection kit, both characterized by use of an organic sulfur reagent, and which are effective at low concentration of the reagent, are inexpensive, and have reduced unpleasent odor. Provided is a reagent kit for detecting a , comprising an organic sulfur reagent having the atomic sequence of sulfur-carbon-sulfur in its chemical structure, a luciferin, adenosine triphosphate and a magnesium ion. Also provide is a method for detecting a , comprising step 1 of mixing an aqueous solution, containing an organic sulfur reagent having the atomic sequence of sulfur-carbon-sulfur in its chemical structure, a luciferin, adenosine triphosphate and a magnesium ion, with a sample containing a , to give a mixed solution; and step 2 of measuring the light emitted in the mixed solution.
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Provided is a reagent kit for detecting a , comprising an organic sulfur reagent having the atomic sequence of sulfur-carbon-sulfur in its chemical structure, a luciferin, adenosine triphosphate and a magnesium ion. Also provide is a method for detecting a , comprising step 1 of mixing an aqueous solution, containing an organic sulfur reagent having the atomic sequence of sulfur-carbon-sulfur in its chemical structure, a luciferin, adenosine triphosphate and a magnesium ion, with a sample containing a , to give a mixed solution; and step 2 of measuring the light emitted in the mixed solution.</abstract><oa>free_for_read</oa></addata></record>
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title Luciferase detection reagent kits and luciferase detection method using the kit
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