Methods for HIV sequencing and genotyping

Methods for HIV sequencing and genotyping

The present invention relates to methods for sequencing the Human Immunodeficiency Virus (HIV) nucleic acids. More specifically, the present invention relates to methods for obtaining information on the genetic sequences of HIV nucleic acid from a patient. That information can be used to genotype a...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Johnston-Dow, Leslie A, Demeter, Lisa, White, Camille B, Song, Keming, Kohlenberger, Robert, Conrad, Morgan, Myers, Angela
Format: Patent
Sprache:eng
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page
container_title
container_volume
creator Johnston-Dow, Leslie A
Demeter, Lisa
White, Camille B
Song, Keming
Kohlenberger, Robert
Conrad, Morgan
Myers, Angela
description The present invention relates to methods for sequencing the Human Immunodeficiency Virus (HIV) nucleic acids. More specifically, the present invention relates to methods for obtaining information on the genetic sequences of HIV nucleic acid from a patient. That information can be used to genotype a HIV quasi-species present in the patient. Methods are provided for amplifying regions of the HIV pol gene amplifying double-stranded nucleic acid template derived from HIV tube RT-PCR with novel PCR primers to produce amplified target sequences. Methods are also provided for analyzing the nucleotide sequence of these amplified targets using novel sequencing primers and the data is analyzed. The determined nucleotide sequence can be compared to the sequence of known drug resistance mutations in the HIV pol gene to determine the viral genotype.
format Patent
fullrecord <record><control><sourceid>uspatents_EFH</sourceid><recordid>TN_cdi_uspatents_grants_06531588</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>06531588</sourcerecordid><originalsourceid>FETCH-uspatents_grants_065315883</originalsourceid><addsrcrecordid>eNrjZND0TS3JyE8pVkjLL1Lw8AxTKE4tLE3NS87MS1dIzEtRSE_Nyy-pLAByeRhY0xJzilN5oTQ3g4Kba4izh25pcUFiSWpeSXF8elEiiDIwMzU2NLWwMCZCCQC2HylR</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Methods for HIV sequencing and genotyping</title><source>USPTO Issued Patents</source><creator>Johnston-Dow, Leslie A ; Demeter, Lisa ; White, Camille B ; Song, Keming ; Kohlenberger, Robert ; Conrad, Morgan ; Myers, Angela</creator><creatorcontrib>Johnston-Dow, Leslie A ; Demeter, Lisa ; White, Camille B ; Song, Keming ; Kohlenberger, Robert ; Conrad, Morgan ; Myers, Angela ; Applera Corporation</creatorcontrib><description>The present invention relates to methods for sequencing the Human Immunodeficiency Virus (HIV) nucleic acids. More specifically, the present invention relates to methods for obtaining information on the genetic sequences of HIV nucleic acid from a patient. That information can be used to genotype a HIV quasi-species present in the patient. Methods are provided for amplifying regions of the HIV pol gene amplifying double-stranded nucleic acid template derived from HIV tube RT-PCR with novel PCR primers to produce amplified target sequences. Methods are also provided for analyzing the nucleotide sequence of these amplified targets using novel sequencing primers and the data is analyzed. The determined nucleotide sequence can be compared to the sequence of known drug resistance mutations in the HIV pol gene to determine the viral genotype.</description><language>eng</language><creationdate>2003</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6531588$$EPDF$$P50$$Guspatents$$Hfree_for_read</linktopdf><link.rule.ids>230,308,776,798,881,64012</link.rule.ids><linktorsrc>$$Uhttps://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6531588$$EView_record_in_USPTO$$FView_record_in_$$GUSPTO$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Johnston-Dow, Leslie A</creatorcontrib><creatorcontrib>Demeter, Lisa</creatorcontrib><creatorcontrib>White, Camille B</creatorcontrib><creatorcontrib>Song, Keming</creatorcontrib><creatorcontrib>Kohlenberger, Robert</creatorcontrib><creatorcontrib>Conrad, Morgan</creatorcontrib><creatorcontrib>Myers, Angela</creatorcontrib><creatorcontrib>Applera Corporation</creatorcontrib><title>Methods for HIV sequencing and genotyping</title><description>The present invention relates to methods for sequencing the Human Immunodeficiency Virus (HIV) nucleic acids. More specifically, the present invention relates to methods for obtaining information on the genetic sequences of HIV nucleic acid from a patient. That information can be used to genotype a HIV quasi-species present in the patient. Methods are provided for amplifying regions of the HIV pol gene amplifying double-stranded nucleic acid template derived from HIV tube RT-PCR with novel PCR primers to produce amplified target sequences. Methods are also provided for analyzing the nucleotide sequence of these amplified targets using novel sequencing primers and the data is analyzed. The determined nucleotide sequence can be compared to the sequence of known drug resistance mutations in the HIV pol gene to determine the viral genotype.</description><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2003</creationdate><recordtype>patent</recordtype><sourceid>EFH</sourceid><recordid>eNrjZND0TS3JyE8pVkjLL1Lw8AxTKE4tLE3NS87MS1dIzEtRSE_Nyy-pLAByeRhY0xJzilN5oTQ3g4Kba4izh25pcUFiSWpeSXF8elEiiDIwMzU2NLWwMCZCCQC2HylR</recordid><startdate>20030311</startdate><enddate>20030311</enddate><creator>Johnston-Dow, Leslie A</creator><creator>Demeter, Lisa</creator><creator>White, Camille B</creator><creator>Song, Keming</creator><creator>Kohlenberger, Robert</creator><creator>Conrad, Morgan</creator><creator>Myers, Angela</creator><scope>EFH</scope></search><sort><creationdate>20030311</creationdate><title>Methods for HIV sequencing and genotyping</title><author>Johnston-Dow, Leslie A ; Demeter, Lisa ; White, Camille B ; Song, Keming ; Kohlenberger, Robert ; Conrad, Morgan ; Myers, Angela</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-uspatents_grants_065315883</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2003</creationdate><toplevel>online_resources</toplevel><creatorcontrib>Johnston-Dow, Leslie A</creatorcontrib><creatorcontrib>Demeter, Lisa</creatorcontrib><creatorcontrib>White, Camille B</creatorcontrib><creatorcontrib>Song, Keming</creatorcontrib><creatorcontrib>Kohlenberger, Robert</creatorcontrib><creatorcontrib>Conrad, Morgan</creatorcontrib><creatorcontrib>Myers, Angela</creatorcontrib><creatorcontrib>Applera Corporation</creatorcontrib><collection>USPTO Issued Patents</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Johnston-Dow, Leslie A</au><au>Demeter, Lisa</au><au>White, Camille B</au><au>Song, Keming</au><au>Kohlenberger, Robert</au><au>Conrad, Morgan</au><au>Myers, Angela</au><aucorp>Applera Corporation</aucorp><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Methods for HIV sequencing and genotyping</title><date>2003-03-11</date><risdate>2003</risdate><abstract>The present invention relates to methods for sequencing the Human Immunodeficiency Virus (HIV) nucleic acids. More specifically, the present invention relates to methods for obtaining information on the genetic sequences of HIV nucleic acid from a patient. That information can be used to genotype a HIV quasi-species present in the patient. Methods are provided for amplifying regions of the HIV pol gene amplifying double-stranded nucleic acid template derived from HIV tube RT-PCR with novel PCR primers to produce amplified target sequences. Methods are also provided for analyzing the nucleotide sequence of these amplified targets using novel sequencing primers and the data is analyzed. The determined nucleotide sequence can be compared to the sequence of known drug resistance mutations in the HIV pol gene to determine the viral genotype.</abstract><oa>free_for_read</oa></addata></record>
fulltext fulltext_linktorsrc
identifier
ispartof
issn
language eng
recordid cdi_uspatents_grants_06531588
source USPTO Issued Patents
title Methods for HIV sequencing and genotyping
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T13%3A13%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-uspatents_EFH&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=Johnston-Dow,%20Leslie%20A&rft.aucorp=Applera%20Corporation&rft.date=2003-03-11&rft_id=info:doi/&rft_dat=%3Cuspatents_EFH%3E06531588%3C/uspatents_EFH%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true