Amino acid sequence of L-phenylalanine ammonia-lyase
1. Field of the Invention In order to provide technical information necessary for the production of L-phenylalanine ammonia-lyase (PAL) by utilizing genetic engineering techniques, the structural gene for PAL and the amino acid sequence of PAL have been elucidated in , and novel recombinant DNA plas...
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creator | Fukuhara, Nobuhiro Yoshino, Setsuo Yamamoto, Kaoru Se, Tomoyuki Sone, Satori Nakajima, Yoshiyuki Suzuki, Maki Makiguchi, Nobuyoshi |
description | 1. Field of the Invention
In order to provide technical information necessary for the production of L-phenylalanine ammonia-lyase (PAL) by utilizing genetic engineering techniques, the structural gene for PAL and the amino acid sequence of PAL have been elucidated in , and novel recombinant DNA plasmids (e.g., pSW101, pYtrp6 and pKY201) have been created by inserting a DNA strand coding for the PAL gene between the 3′-terminus of the promoter region and the 5′-terminus of the terminator region.Moreover, transformants having such a novel recombinant DNA plasmid have been created, and a process for the production of PAL by growing such a novel transformant so as to cause PAL to be produced and accumulated in the culture has been established.Furthermore, there has been established a novel technique for the production of L-phenylalanine by reacting an ammonia donor with cinnamic acid in the presence of the PAL prepared by the aforesaid novel process. |
format | Patent |
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In order to provide technical information necessary for the production of L-phenylalanine ammonia-lyase (PAL) by utilizing genetic engineering techniques, the structural gene for PAL and the amino acid sequence of PAL have been elucidated in , and novel recombinant DNA plasmids (e.g., pSW101, pYtrp6 and pKY201) have been created by inserting a DNA strand coding for the PAL gene between the 3′-terminus of the promoter region and the 5′-terminus of the terminator region.Moreover, transformants having such a novel recombinant DNA plasmid have been created, and a process for the production of PAL by growing such a novel transformant so as to cause PAL to be produced and accumulated in the culture has been established.Furthermore, there has been established a novel technique for the production of L-phenylalanine by reacting an ammonia donor with cinnamic acid in the presence of the PAL prepared by the aforesaid novel process.</description><language>eng</language><creationdate>2002</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6472196$$EPDF$$P50$$Guspatents$$Hfree_for_read</linktopdf><link.rule.ids>230,308,776,798,881,64012</link.rule.ids><linktorsrc>$$Uhttps://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6472196$$EView_record_in_USPTO$$FView_record_in_$$GUSPTO$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Fukuhara, Nobuhiro</creatorcontrib><creatorcontrib>Yoshino, Setsuo</creatorcontrib><creatorcontrib>Yamamoto, Kaoru</creatorcontrib><creatorcontrib>Se, Tomoyuki</creatorcontrib><creatorcontrib>Sone, Satori</creatorcontrib><creatorcontrib>Nakajima, Yoshiyuki</creatorcontrib><creatorcontrib>Suzuki, Maki</creatorcontrib><creatorcontrib>Makiguchi, Nobuyoshi</creatorcontrib><creatorcontrib>Mitsu Chemicals, Inc</creatorcontrib><title>Amino acid sequence of L-phenylalanine ammonia-lyase</title><description>1. Field of the Invention
In order to provide technical information necessary for the production of L-phenylalanine ammonia-lyase (PAL) by utilizing genetic engineering techniques, the structural gene for PAL and the amino acid sequence of PAL have been elucidated in , and novel recombinant DNA plasmids (e.g., pSW101, pYtrp6 and pKY201) have been created by inserting a DNA strand coding for the PAL gene between the 3′-terminus of the promoter region and the 5′-terminus of the terminator region.Moreover, transformants having such a novel recombinant DNA plasmid have been created, and a process for the production of PAL by growing such a novel transformant so as to cause PAL to be produced and accumulated in the culture has been established.Furthermore, there has been established a novel technique for the production of L-phenylalanine by reacting an ammonia donor with cinnamic acid in the presence of the PAL prepared by the aforesaid novel process.</description><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2002</creationdate><recordtype>patent</recordtype><sourceid>EFH</sourceid><recordid>eNrjZDBxzM3My1dITM5MUShOLSxNzUtOVchPU_DRLchIzavMScxJzMvMS1VIzM3Nz8tM1M2pTCxO5WFgTUvMKU7lhdLcDApuriHOHrqlxQWJJal5JcXx6UWJIMrAzMTcyNDSzJgIJQDcTy1w</recordid><startdate>20021029</startdate><enddate>20021029</enddate><creator>Fukuhara, Nobuhiro</creator><creator>Yoshino, Setsuo</creator><creator>Yamamoto, Kaoru</creator><creator>Se, Tomoyuki</creator><creator>Sone, Satori</creator><creator>Nakajima, Yoshiyuki</creator><creator>Suzuki, Maki</creator><creator>Makiguchi, Nobuyoshi</creator><scope>EFH</scope></search><sort><creationdate>20021029</creationdate><title>Amino acid sequence of L-phenylalanine ammonia-lyase</title><author>Fukuhara, Nobuhiro ; Yoshino, Setsuo ; Yamamoto, Kaoru ; Se, Tomoyuki ; Sone, Satori ; Nakajima, Yoshiyuki ; Suzuki, Maki ; Makiguchi, Nobuyoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-uspatents_grants_064721963</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2002</creationdate><toplevel>online_resources</toplevel><creatorcontrib>Fukuhara, Nobuhiro</creatorcontrib><creatorcontrib>Yoshino, Setsuo</creatorcontrib><creatorcontrib>Yamamoto, Kaoru</creatorcontrib><creatorcontrib>Se, Tomoyuki</creatorcontrib><creatorcontrib>Sone, Satori</creatorcontrib><creatorcontrib>Nakajima, Yoshiyuki</creatorcontrib><creatorcontrib>Suzuki, Maki</creatorcontrib><creatorcontrib>Makiguchi, Nobuyoshi</creatorcontrib><creatorcontrib>Mitsu Chemicals, Inc</creatorcontrib><collection>USPTO Issued Patents</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Fukuhara, Nobuhiro</au><au>Yoshino, Setsuo</au><au>Yamamoto, Kaoru</au><au>Se, Tomoyuki</au><au>Sone, Satori</au><au>Nakajima, Yoshiyuki</au><au>Suzuki, Maki</au><au>Makiguchi, Nobuyoshi</au><aucorp>Mitsu Chemicals, Inc</aucorp><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Amino acid sequence of L-phenylalanine ammonia-lyase</title><date>2002-10-29</date><risdate>2002</risdate><abstract>1. Field of the Invention
In order to provide technical information necessary for the production of L-phenylalanine ammonia-lyase (PAL) by utilizing genetic engineering techniques, the structural gene for PAL and the amino acid sequence of PAL have been elucidated in , and novel recombinant DNA plasmids (e.g., pSW101, pYtrp6 and pKY201) have been created by inserting a DNA strand coding for the PAL gene between the 3′-terminus of the promoter region and the 5′-terminus of the terminator region.Moreover, transformants having such a novel recombinant DNA plasmid have been created, and a process for the production of PAL by growing such a novel transformant so as to cause PAL to be produced and accumulated in the culture has been established.Furthermore, there has been established a novel technique for the production of L-phenylalanine by reacting an ammonia donor with cinnamic acid in the presence of the PAL prepared by the aforesaid novel process.</abstract><oa>free_for_read</oa></addata></record> |
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title | Amino acid sequence of L-phenylalanine ammonia-lyase |
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