Method for producing a recombinant protein

Method for producing a recombinant protein

The present invention relates to methods for producing recombinant proteins, in particular recombinant secretory proteins, to a method for identifying nucleic acid molecules the expression products of which permit improved secretion of a recombinant secretory protein, to the use of molecules thus id...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: London, Markus, Farwick, Mike, Dohmen, Juergen, Dahlems, Ulrike, Gellissen, Gerd, Strasser, Alexander
Format: Patent
Sprache:eng
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page
container_title
container_volume
creator London, Markus
Farwick, Mike
Dohmen, Juergen
Dahlems, Ulrike
Gellissen, Gerd
Strasser, Alexander
description The present invention relates to methods for producing recombinant proteins, in particular recombinant secretory proteins, to a method for identifying nucleic acid molecules the expression products of which permit improved secretion of a recombinant secretory protein, to the use of molecules thus identified for enhancing secretion of heterologous proteins, and to corresponding kit systems. The method according to the invention makes provision for expression in a suitable host cell of a nucleic acid coding for a secretory protein together with a nucleic acid coding for a polypeptide having the biological activity of the eukaryotic translation initiation factor 4 E and/or for a polypeptide having the biological activity of a CaM kinase. It was found that coexpression of the translation initiation factor gene and/or of the CaM kinase gene leads to improved secretion of a desired recombinant protein from the cell.
format Patent
fullrecord <record><control><sourceid>uspatents_EFI</sourceid><recordid>TN_cdi_uspatents_applications_20030040047</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>20030040047</sourcerecordid><originalsourceid>FETCH-uspatents_applications_200300400473</originalsourceid><addsrcrecordid>eNrjZNDyTS3JyE9RSMsvUigoyk8pTc7MS1dIVChKTc7PTcrMS8wrAYmXpGbm8TCwpiXmFKfyQmluBk031xBnD93S4oLEktS8kuL4xIKCnMzkxJLM_LzieCMDA2MDAxMgMjcmRS0AYZAwEA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Method for producing a recombinant protein</title><source>USPTO Published Applications</source><creator>London, Markus ; Farwick, Mike ; Dohmen, Juergen ; Dahlems, Ulrike ; Gellissen, Gerd ; Strasser, Alexander</creator><creatorcontrib>London, Markus ; Farwick, Mike ; Dohmen, Juergen ; Dahlems, Ulrike ; Gellissen, Gerd ; Strasser, Alexander</creatorcontrib><description>The present invention relates to methods for producing recombinant proteins, in particular recombinant secretory proteins, to a method for identifying nucleic acid molecules the expression products of which permit improved secretion of a recombinant secretory protein, to the use of molecules thus identified for enhancing secretion of heterologous proteins, and to corresponding kit systems. The method according to the invention makes provision for expression in a suitable host cell of a nucleic acid coding for a secretory protein together with a nucleic acid coding for a polypeptide having the biological activity of the eukaryotic translation initiation factor 4 E and/or for a polypeptide having the biological activity of a CaM kinase. It was found that coexpression of the translation initiation factor gene and/or of the CaM kinase gene leads to improved secretion of a desired recombinant protein from the cell.</description><language>eng</language><creationdate>2003</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20030040047$$EPDF$$P50$$Guspatents$$Hfree_for_read</linktopdf><link.rule.ids>230,308,780,873,885,64057</link.rule.ids><linktorsrc>$$Uhttps://patentcenter.uspto.gov/applications/09999392$$EView_record_in_USPTO$$FView_record_in_$$GUSPTO$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>London, Markus</creatorcontrib><creatorcontrib>Farwick, Mike</creatorcontrib><creatorcontrib>Dohmen, Juergen</creatorcontrib><creatorcontrib>Dahlems, Ulrike</creatorcontrib><creatorcontrib>Gellissen, Gerd</creatorcontrib><creatorcontrib>Strasser, Alexander</creatorcontrib><title>Method for producing a recombinant protein</title><description>The present invention relates to methods for producing recombinant proteins, in particular recombinant secretory proteins, to a method for identifying nucleic acid molecules the expression products of which permit improved secretion of a recombinant secretory protein, to the use of molecules thus identified for enhancing secretion of heterologous proteins, and to corresponding kit systems. The method according to the invention makes provision for expression in a suitable host cell of a nucleic acid coding for a secretory protein together with a nucleic acid coding for a polypeptide having the biological activity of the eukaryotic translation initiation factor 4 E and/or for a polypeptide having the biological activity of a CaM kinase. It was found that coexpression of the translation initiation factor gene and/or of the CaM kinase gene leads to improved secretion of a desired recombinant protein from the cell.</description><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2003</creationdate><recordtype>patent</recordtype><sourceid>EFI</sourceid><recordid>eNrjZNDyTS3JyE9RSMsvUigoyk8pTc7MS1dIVChKTc7PTcrMS8wrAYmXpGbm8TCwpiXmFKfyQmluBk031xBnD93S4oLEktS8kuL4xIKCnMzkxJLM_LzieCMDA2MDAxMgMjcmRS0AYZAwEA</recordid><startdate>20030227</startdate><enddate>20030227</enddate><creator>London, Markus</creator><creator>Farwick, Mike</creator><creator>Dohmen, Juergen</creator><creator>Dahlems, Ulrike</creator><creator>Gellissen, Gerd</creator><creator>Strasser, Alexander</creator><scope>EFI</scope></search><sort><creationdate>20030227</creationdate><title>Method for producing a recombinant protein</title><author>London, Markus ; Farwick, Mike ; Dohmen, Juergen ; Dahlems, Ulrike ; Gellissen, Gerd ; Strasser, Alexander</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-uspatents_applications_200300400473</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2003</creationdate><toplevel>online_resources</toplevel><creatorcontrib>London, Markus</creatorcontrib><creatorcontrib>Farwick, Mike</creatorcontrib><creatorcontrib>Dohmen, Juergen</creatorcontrib><creatorcontrib>Dahlems, Ulrike</creatorcontrib><creatorcontrib>Gellissen, Gerd</creatorcontrib><creatorcontrib>Strasser, Alexander</creatorcontrib><collection>USPTO Published Applications</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>London, Markus</au><au>Farwick, Mike</au><au>Dohmen, Juergen</au><au>Dahlems, Ulrike</au><au>Gellissen, Gerd</au><au>Strasser, Alexander</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for producing a recombinant protein</title><date>2003-02-27</date><risdate>2003</risdate><abstract>The present invention relates to methods for producing recombinant proteins, in particular recombinant secretory proteins, to a method for identifying nucleic acid molecules the expression products of which permit improved secretion of a recombinant secretory protein, to the use of molecules thus identified for enhancing secretion of heterologous proteins, and to corresponding kit systems. The method according to the invention makes provision for expression in a suitable host cell of a nucleic acid coding for a secretory protein together with a nucleic acid coding for a polypeptide having the biological activity of the eukaryotic translation initiation factor 4 E and/or for a polypeptide having the biological activity of a CaM kinase. It was found that coexpression of the translation initiation factor gene and/or of the CaM kinase gene leads to improved secretion of a desired recombinant protein from the cell.</abstract><oa>free_for_read</oa></addata></record>
fulltext fulltext_linktorsrc
identifier
ispartof
issn
language eng
recordid cdi_uspatents_applications_20030040047
source USPTO Published Applications
title Method for producing a recombinant protein
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-09T20%3A21%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-uspatents_EFI&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=London,%20Markus&rft.date=2003-02-27&rft_id=info:doi/&rft_dat=%3Cuspatents_EFI%3E20030040047%3C/uspatents_EFI%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true