Expression of the β-(1,3-1,4)-glucanase gene in streptococcus salivarius subsp. thermophilus
Bu çalışmada, kanatlılara yönelik rekombinant probiyotik geliştirmek amacıyla β-(1,3-1,4)-glukanaz (likenaz) geninin Streptococcus salivarius subsp. thermophilus'a aktarılması, ekspresyonu ve likenaz enziminin sıcaklığa direncinin artırılması amaçlanmıştır. β-(1,3-1,4)-glukana...
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| Veröffentlicht in: | Turkish journal of veterinary & animal sciences 2007, Vol.31 (5), p.319-324 |
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| creator | ÖZCAN, Numan AŞAN, Meltem |
| description | Bu çalışmada, kanatlılara yönelik rekombinant probiyotik geliştirmek amacıyla β-(1,3-1,4)-glukanaz (likenaz) geninin
Streptococcus salivarius subsp. thermophilus'a aktarılması, ekspresyonu ve likenaz enziminin sıcaklığa direncinin artırılması
amaçlanmıştır. β-(1,3-1,4)-glukanaz genini taşıyan rekombinant TL1R plazmidi S. salivarius subsp. thermophilus'a
elektrotransformasyon yöntemi ile aktarılmıştır. β-(1,3-1,4)-glukanaz geninin S. salivarius subsp. thermophilus'da ekspresyonu
likenanlı besiyeri, SDS-PAGE ve zymogram analizleri ile tespit edilmiştir. S. salivarius subsp. thermophilus'un sentezlediği β-(1,3-
1,4)-glukanaz enzimi, sıcaklığa direnci artarak 70 ºC'de 15 dakika aktivitesini korumuştur. Buna karşılık, Lactococcus lactis ve
Escherichia coli tarafından sentezlenen β-(1,3-1,4)-glukanaz enzimi aynı sıcaklık uygulamasında aktivitesini kaybetmiştir. Tüm
rekombinant bakterilerce sentezlenen β-(1,3-1,4)-glukanaz enzimi 37-100 ºC'lerde 15 dakika sıcaklık uygulamasından sonra
denatürasyona direnerek bir şekilde suda çözünürlüğünü kaybetmemiştir.
The purpose of this study was the transformation and expression of the β-(1,3-1,4)-glucanase (lichenase) gene in
Streptococcus salivarius subsp. thermophilus to create a recombinant probiotic for poultry and improve the thermostability of the
lichenase enzyme. The recombinant plasmid TL1R containing the β-(1,3-1,4)-glucanase gene was introduced into S. salivarius subsp.
thermophilus by electrotransformation. The expressing of the β-(1,3-1,4)-glucanase gene in S. salivarius subsp. thermophilus was
confirmed on lichenan plate, SDS-PAGE, and zymogram analysis. The β-(1,3-1,4)-glucanase enzyme expressed by S. salivarius subsp.
thermophilus cells seemed to increase its capacity for thermoresistance and so it maintained its activity at 70 ºC for 15 min. In
contrast, the enzyme produced by Lactococcus lactis and Escherichia coli cells easily ceased activity when exposed to the same
temperature. The enzyme expressed by all the recombinant bacteria resisted denaturation and somehow remained soluble after heat
treatment from 37 to 100 ºC for 15 min. |
| format | Article |
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Streptococcus salivarius subsp. thermophilus'a aktarılması, ekspresyonu ve likenaz enziminin sıcaklığa direncinin artırılması
amaçlanmıştır. β-(1,3-1,4)-glukanaz genini taşıyan rekombinant TL1R plazmidi S. salivarius subsp. thermophilus'a
elektrotransformasyon yöntemi ile aktarılmıştır. β-(1,3-1,4)-glukanaz geninin S. salivarius subsp. thermophilus'da ekspresyonu
likenanlı besiyeri, SDS-PAGE ve zymogram analizleri ile tespit edilmiştir. S. salivarius subsp. thermophilus'un sentezlediği β-(1,3-
1,4)-glukanaz enzimi, sıcaklığa direnci artarak 70 ºC'de 15 dakika aktivitesini korumuştur. Buna karşılık, Lactococcus lactis ve
Escherichia coli tarafından sentezlenen β-(1,3-1,4)-glukanaz enzimi aynı sıcaklık uygulamasında aktivitesini kaybetmiştir. Tüm
rekombinant bakterilerce sentezlenen β-(1,3-1,4)-glukanaz enzimi 37-100 ºC'lerde 15 dakika sıcaklık uygulamasından sonra
denatürasyona direnerek bir şekilde suda çözünürlüğünü kaybetmemiştir.
The purpose of this study was the transformation and expression of the β-(1,3-1,4)-glucanase (lichenase) gene in
Streptococcus salivarius subsp. thermophilus to create a recombinant probiotic for poultry and improve the thermostability of the
lichenase enzyme. The recombinant plasmid TL1R containing the β-(1,3-1,4)-glucanase gene was introduced into S. salivarius subsp.
thermophilus by electrotransformation. The expressing of the β-(1,3-1,4)-glucanase gene in S. salivarius subsp. thermophilus was
confirmed on lichenan plate, SDS-PAGE, and zymogram analysis. The β-(1,3-1,4)-glucanase enzyme expressed by S. salivarius subsp.
thermophilus cells seemed to increase its capacity for thermoresistance and so it maintained its activity at 70 ºC for 15 min. In
contrast, the enzyme produced by Lactococcus lactis and Escherichia coli cells easily ceased activity when exposed to the same
temperature. The enzyme expressed by all the recombinant bacteria resisted denaturation and somehow remained soluble after heat
treatment from 37 to 100 ºC for 15 min.]]></description><identifier>ISSN: 1300-0128</identifier><language>eng</language><publisher>TÜBİTAK</publisher><subject>Animal Nutrition (Physiology) ; denaturasyon ; denaturation ; Escherichia ; Escherichia coli ; gen ifadesi ; gene expression ; genes ; Genetics and Molecular Biology of Microorganisms ; genler ; Hayvan Besleme (Fizyoloji) ; heat stability ; ısı kararlılığı ; kümes hayvanları ; Lactococcus lactis ; Mikroorganizmaların Genetiği ve Moleküler Biyolojisi ; plasmids ; plazmitler ; poultry ; probiotics ; probiyotikler ; recombination ; rekombinasyon ; Streptococcus thermophilus</subject><ispartof>Turkish journal of veterinary & animal sciences, 2007, Vol.31 (5), p.319-324</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010</link.rule.ids></links><search><creatorcontrib>ÖZCAN, Numan</creatorcontrib><creatorcontrib>AŞAN, Meltem</creatorcontrib><title>Expression of the &#946;-(1,3-1,4)-glucanase gene in streptococcus salivarius subsp. thermophilus</title><title>Turkish journal of veterinary & animal sciences</title><description><![CDATA[Bu çalışmada, kanatlılara yönelik rekombinant probiyotik geliştirmek amacıyla β-(1,3-1,4)-glukanaz (likenaz) geninin
Streptococcus salivarius subsp. thermophilus'a aktarılması, ekspresyonu ve likenaz enziminin sıcaklığa direncinin artırılması
amaçlanmıştır. β-(1,3-1,4)-glukanaz genini taşıyan rekombinant TL1R plazmidi S. salivarius subsp. thermophilus'a
elektrotransformasyon yöntemi ile aktarılmıştır. β-(1,3-1,4)-glukanaz geninin S. salivarius subsp. thermophilus'da ekspresyonu
likenanlı besiyeri, SDS-PAGE ve zymogram analizleri ile tespit edilmiştir. S. salivarius subsp. thermophilus'un sentezlediği β-(1,3-
1,4)-glukanaz enzimi, sıcaklığa direnci artarak 70 ºC'de 15 dakika aktivitesini korumuştur. Buna karşılık, Lactococcus lactis ve
Escherichia coli tarafından sentezlenen β-(1,3-1,4)-glukanaz enzimi aynı sıcaklık uygulamasında aktivitesini kaybetmiştir. Tüm
rekombinant bakterilerce sentezlenen β-(1,3-1,4)-glukanaz enzimi 37-100 ºC'lerde 15 dakika sıcaklık uygulamasından sonra
denatürasyona direnerek bir şekilde suda çözünürlüğünü kaybetmemiştir.
The purpose of this study was the transformation and expression of the β-(1,3-1,4)-glucanase (lichenase) gene in
Streptococcus salivarius subsp. thermophilus to create a recombinant probiotic for poultry and improve the thermostability of the
lichenase enzyme. The recombinant plasmid TL1R containing the β-(1,3-1,4)-glucanase gene was introduced into S. salivarius subsp.
thermophilus by electrotransformation. The expressing of the β-(1,3-1,4)-glucanase gene in S. salivarius subsp. thermophilus was
confirmed on lichenan plate, SDS-PAGE, and zymogram analysis. The β-(1,3-1,4)-glucanase enzyme expressed by S. salivarius subsp.
thermophilus cells seemed to increase its capacity for thermoresistance and so it maintained its activity at 70 ºC for 15 min. In
contrast, the enzyme produced by Lactococcus lactis and Escherichia coli cells easily ceased activity when exposed to the same
temperature. The enzyme expressed by all the recombinant bacteria resisted denaturation and somehow remained soluble after heat
treatment from 37 to 100 ºC for 15 min.]]></description><subject>Animal Nutrition (Physiology)</subject><subject>denaturasyon</subject><subject>denaturation</subject><subject>Escherichia</subject><subject>Escherichia coli</subject><subject>gen ifadesi</subject><subject>gene expression</subject><subject>genes</subject><subject>Genetics and Molecular Biology of Microorganisms</subject><subject>genler</subject><subject>Hayvan Besleme (Fizyoloji)</subject><subject>heat stability</subject><subject>ısı kararlılığı</subject><subject>kümes hayvanları</subject><subject>Lactococcus lactis</subject><subject>Mikroorganizmaların Genetiği ve Moleküler Biyolojisi</subject><subject>plasmids</subject><subject>plazmitler</subject><subject>poultry</subject><subject>probiotics</subject><subject>probiyotikler</subject><subject>recombination</subject><subject>rekombinasyon</subject><subject>Streptococcus thermophilus</subject><issn>1300-0128</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNqFjL0KwjAYADMoWH_ewCEgiEIjSVOrxVEUH8C9pCFto2kT8jWiby8Fd6c7OLgRihinlFCWHCdoCvCgNOFpRiMkLm_nFYC2HbYV7huF16s8zU5kw2JOWJxuSW2CFJ0AhWvVKaw7DL1XrrfSShkAgzD6JbweNJTgdsPGt9Y12gSYo3ElDKjFjzO0vF7u5xsJRjxL3RbO61b4T3HI8nzP_-Qvlwk-iA</recordid><startdate>2007</startdate><enddate>2007</enddate><creator>ÖZCAN, Numan</creator><creator>AŞAN, Meltem</creator><general>TÜBİTAK</general><scope/></search><sort><creationdate>2007</creationdate><title>Expression of the &#946;-(1,3-1,4)-glucanase gene in streptococcus salivarius subsp. thermophilus</title><author>ÖZCAN, Numan ; AŞAN, Meltem</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-ulakbim_primary_769953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animal Nutrition (Physiology)</topic><topic>denaturasyon</topic><topic>denaturation</topic><topic>Escherichia</topic><topic>Escherichia coli</topic><topic>gen ifadesi</topic><topic>gene expression</topic><topic>genes</topic><topic>Genetics and Molecular Biology of Microorganisms</topic><topic>genler</topic><topic>Hayvan Besleme (Fizyoloji)</topic><topic>heat stability</topic><topic>ısı kararlılığı</topic><topic>kümes hayvanları</topic><topic>Lactococcus lactis</topic><topic>Mikroorganizmaların Genetiği ve Moleküler Biyolojisi</topic><topic>plasmids</topic><topic>plazmitler</topic><topic>poultry</topic><topic>probiotics</topic><topic>probiyotikler</topic><topic>recombination</topic><topic>rekombinasyon</topic><topic>Streptococcus thermophilus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ÖZCAN, Numan</creatorcontrib><creatorcontrib>AŞAN, Meltem</creatorcontrib><jtitle>Turkish journal of veterinary & animal sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ÖZCAN, Numan</au><au>AŞAN, Meltem</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of the &#946;-(1,3-1,4)-glucanase gene in streptococcus salivarius subsp. thermophilus</atitle><jtitle>Turkish journal of veterinary & animal sciences</jtitle><date>2007</date><risdate>2007</risdate><volume>31</volume><issue>5</issue><spage>319</spage><epage>324</epage><pages>319-324</pages><issn>1300-0128</issn><abstract><![CDATA[Bu çalışmada, kanatlılara yönelik rekombinant probiyotik geliştirmek amacıyla β-(1,3-1,4)-glukanaz (likenaz) geninin
Streptococcus salivarius subsp. thermophilus'a aktarılması, ekspresyonu ve likenaz enziminin sıcaklığa direncinin artırılması
amaçlanmıştır. β-(1,3-1,4)-glukanaz genini taşıyan rekombinant TL1R plazmidi S. salivarius subsp. thermophilus'a
elektrotransformasyon yöntemi ile aktarılmıştır. β-(1,3-1,4)-glukanaz geninin S. salivarius subsp. thermophilus'da ekspresyonu
likenanlı besiyeri, SDS-PAGE ve zymogram analizleri ile tespit edilmiştir. S. salivarius subsp. thermophilus'un sentezlediği β-(1,3-
1,4)-glukanaz enzimi, sıcaklığa direnci artarak 70 ºC'de 15 dakika aktivitesini korumuştur. Buna karşılık, Lactococcus lactis ve
Escherichia coli tarafından sentezlenen β-(1,3-1,4)-glukanaz enzimi aynı sıcaklık uygulamasında aktivitesini kaybetmiştir. Tüm
rekombinant bakterilerce sentezlenen β-(1,3-1,4)-glukanaz enzimi 37-100 ºC'lerde 15 dakika sıcaklık uygulamasından sonra
denatürasyona direnerek bir şekilde suda çözünürlüğünü kaybetmemiştir.
The purpose of this study was the transformation and expression of the β-(1,3-1,4)-glucanase (lichenase) gene in
Streptococcus salivarius subsp. thermophilus to create a recombinant probiotic for poultry and improve the thermostability of the
lichenase enzyme. The recombinant plasmid TL1R containing the β-(1,3-1,4)-glucanase gene was introduced into S. salivarius subsp.
thermophilus by electrotransformation. The expressing of the β-(1,3-1,4)-glucanase gene in S. salivarius subsp. thermophilus was
confirmed on lichenan plate, SDS-PAGE, and zymogram analysis. The β-(1,3-1,4)-glucanase enzyme expressed by S. salivarius subsp.
thermophilus cells seemed to increase its capacity for thermoresistance and so it maintained its activity at 70 ºC for 15 min. In
contrast, the enzyme produced by Lactococcus lactis and Escherichia coli cells easily ceased activity when exposed to the same
temperature. The enzyme expressed by all the recombinant bacteria resisted denaturation and somehow remained soluble after heat
treatment from 37 to 100 ºC for 15 min.]]></abstract><pub>TÜBİTAK</pub><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1300-0128 |
| ispartof | Turkish journal of veterinary & animal sciences, 2007, Vol.31 (5), p.319-324 |
| issn | 1300-0128 |
| language | eng |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; TÜBİTAK Scientific Journals |
| subjects | Animal Nutrition (Physiology) denaturasyon denaturation Escherichia Escherichia coli gen ifadesi gene expression genes Genetics and Molecular Biology of Microorganisms genler Hayvan Besleme (Fizyoloji) heat stability ısı kararlılığı kümes hayvanları Lactococcus lactis Mikroorganizmaların Genetiği ve Moleküler Biyolojisi plasmids plazmitler poultry probiotics probiyotikler recombination rekombinasyon Streptococcus thermophilus |
| title | Expression of the β-(1,3-1,4)-glucanase gene in streptococcus salivarius subsp. thermophilus |
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