Coagulase gene polymorphism of staphylococcus aurreus isolated from subclinical bovine mastitis
Özet: Bu çalışma, üç farklı şehirden (Hatay, Gaziantep ve Burdur) toplanan mastitisli sığır sütlerinden izole edilen 80 Staphylococcus aureus susunun koagulaz gen polimorfizmini araştırmak amacıyla yapıldı. Seksen S. aureus susu 5 farklı polymerase chain reaction (PCR) amplikonu oluştururken, 79 izo...
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| Veröffentlicht in: | Turkish journal of veterinary & animal sciences 2007, Vol.31 (4), p.253-257 |
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| container_title | Turkish journal of veterinary & animal sciences |
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| creator | DEMİR, Cemil DOĞRUER, Gökhan EGÜN, Yaşar TÜRÜTOĞLU, Hülya CANTEKİN, Zafer ASLANTAŞ, Özkan |
| description | Özet: Bu çalışma, üç farklı şehirden (Hatay, Gaziantep ve Burdur) toplanan mastitisli sığır sütlerinden izole edilen 80 Staphylococcus
aureus susunun koagulaz gen polimorfizmini araştırmak amacıyla yapıldı. Seksen S. aureus susu 5 farklı polymerase chain reaction
(PCR) amplikonu oluştururken, 79 izolat 1 amplikon ve 1 izolat 2 amplikon oluşturdu. İzolatlar, koagulaz geninin restriction
fragment length polymorphism (RFLP) analizi ile 9 genotipe ayrıldı. İki yaygın genotip izolatların % 73,8'ini oluşturdu. Sonuçlar,
çalışılan bölgelerde farklı genotiplerin olduğunu ve bunlardan sadece bir veya iki genotipin dominant olduğunu göstermektedir
Abstract: This study was conducted to investigate the coagulase gene polymorphism of Staphylococcus aureus isolated from
subclinical bovine mastitic milk samples from 3 different Turkish cities (Hatay, Gaziantep, and Burdur). Amplification of the
coagulase gene from 80 5. aureus isolates produced 5 different polymerase chain reaction (PCR) products; 79 isolates showed only
1 amplicon, and 1 isolate showed 2 amplicons. The isolates were grouped into 9 genotypes by analysing the restriction fragment
length polymorphism (RFLP) of the gene; the 2 most common genotypes accounted for 73.8% of the isolates. The results indicated
that many coagulase gene genotypes are present in the studied regions and only 1 or 2 genotypes predominated. |
| format | Article |
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aureus susunun koagulaz gen polimorfizmini araştırmak amacıyla yapıldı. Seksen S. aureus susu 5 farklı polymerase chain reaction
(PCR) amplikonu oluştururken, 79 izolat 1 amplikon ve 1 izolat 2 amplikon oluşturdu. İzolatlar, koagulaz geninin restriction
fragment length polymorphism (RFLP) analizi ile 9 genotipe ayrıldı. İki yaygın genotip izolatların % 73,8'ini oluşturdu. Sonuçlar,
çalışılan bölgelerde farklı genotiplerin olduğunu ve bunlardan sadece bir veya iki genotipin dominant olduğunu göstermektedir
Abstract: This study was conducted to investigate the coagulase gene polymorphism of Staphylococcus aureus isolated from
subclinical bovine mastitic milk samples from 3 different Turkish cities (Hatay, Gaziantep, and Burdur). Amplification of the
coagulase gene from 80 5. aureus isolates produced 5 different polymerase chain reaction (PCR) products; 79 isolates showed only
1 amplicon, and 1 isolate showed 2 amplicons. The isolates were grouped into 9 genotypes by analysing the restriction fragment
length polymorphism (RFLP) of the gene; the 2 most common genotypes accounted for 73.8% of the isolates. The results indicated
that many coagulase gene genotypes are present in the studied regions and only 1 or 2 genotypes predominated.</description><identifier>ISSN: 1300-0128</identifier><language>eng</language><publisher>TÜBİTAK</publisher><subject>bovine mastitis ; cattle ; Dairy Animals ; genes ; genetic polymorphism ; Genetics and Molecular Biology of Microorganisms ; genetik polimorfizm ; genler ; genotip ; genotypes ; Hayvanların Prion, Viral, Bakteriyel ve Fungal Patojenleri ; kısıtlı fragment uzunluğu polimorfizmi ; mastitis ; meme yangısı ; Mikroorganizmaların Genetiği ve Moleküler Biyolojisi ; Prion, Viral, Bacterial and Fungal Pathogens of Animals ; restriction fragment length polymorphism ; RFLP ; sığır ; sığır meme yangısı ; Staphylococcus aureus ; subclinical mastitis ; subklinik meme yangısı ; Süt Hayvanları</subject><ispartof>Turkish journal of veterinary & animal sciences, 2007, Vol.31 (4), p.253-257</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4009</link.rule.ids></links><search><creatorcontrib>DEMİR, Cemil</creatorcontrib><creatorcontrib>DOĞRUER, Gökhan</creatorcontrib><creatorcontrib>EGÜN, Yaşar</creatorcontrib><creatorcontrib>TÜRÜTOĞLU, Hülya</creatorcontrib><creatorcontrib>CANTEKİN, Zafer</creatorcontrib><creatorcontrib>ASLANTAŞ, Özkan</creatorcontrib><title>Coagulase gene polymorphism of staphylococcus aurreus isolated from subclinical bovine mastitis</title><title>Turkish journal of veterinary & animal sciences</title><description>Özet: Bu çalışma, üç farklı şehirden (Hatay, Gaziantep ve Burdur) toplanan mastitisli sığır sütlerinden izole edilen 80 Staphylococcus
aureus susunun koagulaz gen polimorfizmini araştırmak amacıyla yapıldı. Seksen S. aureus susu 5 farklı polymerase chain reaction
(PCR) amplikonu oluştururken, 79 izolat 1 amplikon ve 1 izolat 2 amplikon oluşturdu. İzolatlar, koagulaz geninin restriction
fragment length polymorphism (RFLP) analizi ile 9 genotipe ayrıldı. İki yaygın genotip izolatların % 73,8'ini oluşturdu. Sonuçlar,
çalışılan bölgelerde farklı genotiplerin olduğunu ve bunlardan sadece bir veya iki genotipin dominant olduğunu göstermektedir
Abstract: This study was conducted to investigate the coagulase gene polymorphism of Staphylococcus aureus isolated from
subclinical bovine mastitic milk samples from 3 different Turkish cities (Hatay, Gaziantep, and Burdur). Amplification of the
coagulase gene from 80 5. aureus isolates produced 5 different polymerase chain reaction (PCR) products; 79 isolates showed only
1 amplicon, and 1 isolate showed 2 amplicons. The isolates were grouped into 9 genotypes by analysing the restriction fragment
length polymorphism (RFLP) of the gene; the 2 most common genotypes accounted for 73.8% of the isolates. The results indicated
that many coagulase gene genotypes are present in the studied regions and only 1 or 2 genotypes predominated.</description><subject>bovine mastitis</subject><subject>cattle</subject><subject>Dairy Animals</subject><subject>genes</subject><subject>genetic polymorphism</subject><subject>Genetics and Molecular Biology of Microorganisms</subject><subject>genetik polimorfizm</subject><subject>genler</subject><subject>genotip</subject><subject>genotypes</subject><subject>Hayvanların Prion, Viral, Bakteriyel ve Fungal Patojenleri</subject><subject>kısıtlı fragment uzunluğu polimorfizmi</subject><subject>mastitis</subject><subject>meme yangısı</subject><subject>Mikroorganizmaların Genetiği ve Moleküler Biyolojisi</subject><subject>Prion, Viral, Bacterial and Fungal Pathogens of Animals</subject><subject>restriction fragment length polymorphism</subject><subject>RFLP</subject><subject>sığır</subject><subject>sığır meme yangısı</subject><subject>Staphylococcus aureus</subject><subject>subclinical mastitis</subject><subject>subklinik meme yangısı</subject><subject>Süt Hayvanları</subject><issn>1300-0128</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNqFjUsOgjAUALvQRPzcwEUvQFKsAfdE4wHck0ct8LTlkb7WhNvLwr2rWUwysxJZoZXKVXG6bMSW-aXUSZ9LlYmmJuiTA7ayt6OVE7nZU5gGZC-pkxxhGmZHhoxJLCGFYBcik4Non7IL5CWn1jgc0YCTLX1w6XjgiBF5L9YdOLaHH3fieLs-6nu-PN8t-mYK6CHMTVXqqtJ_9Bc5wEFx</recordid><startdate>2007</startdate><enddate>2007</enddate><creator>DEMİR, Cemil</creator><creator>DOĞRUER, Gökhan</creator><creator>EGÜN, Yaşar</creator><creator>TÜRÜTOĞLU, Hülya</creator><creator>CANTEKİN, Zafer</creator><creator>ASLANTAŞ, Özkan</creator><general>TÜBİTAK</general><scope/></search><sort><creationdate>2007</creationdate><title>Coagulase gene polymorphism of staphylococcus aurreus isolated from subclinical bovine mastitis</title><author>DEMİR, Cemil ; DOĞRUER, Gökhan ; EGÜN, Yaşar ; TÜRÜTOĞLU, Hülya ; CANTEKİN, Zafer ; ASLANTAŞ, Özkan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-ulakbim_primary_763773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>bovine mastitis</topic><topic>cattle</topic><topic>Dairy Animals</topic><topic>genes</topic><topic>genetic polymorphism</topic><topic>Genetics and Molecular Biology of Microorganisms</topic><topic>genetik polimorfizm</topic><topic>genler</topic><topic>genotip</topic><topic>genotypes</topic><topic>Hayvanların Prion, Viral, Bakteriyel ve Fungal Patojenleri</topic><topic>kısıtlı fragment uzunluğu polimorfizmi</topic><topic>mastitis</topic><topic>meme yangısı</topic><topic>Mikroorganizmaların Genetiği ve Moleküler Biyolojisi</topic><topic>Prion, Viral, Bacterial and Fungal Pathogens of Animals</topic><topic>restriction fragment length polymorphism</topic><topic>RFLP</topic><topic>sığır</topic><topic>sığır meme yangısı</topic><topic>Staphylococcus aureus</topic><topic>subclinical mastitis</topic><topic>subklinik meme yangısı</topic><topic>Süt Hayvanları</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DEMİR, Cemil</creatorcontrib><creatorcontrib>DOĞRUER, Gökhan</creatorcontrib><creatorcontrib>EGÜN, Yaşar</creatorcontrib><creatorcontrib>TÜRÜTOĞLU, Hülya</creatorcontrib><creatorcontrib>CANTEKİN, Zafer</creatorcontrib><creatorcontrib>ASLANTAŞ, Özkan</creatorcontrib><jtitle>Turkish journal of veterinary & animal sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DEMİR, Cemil</au><au>DOĞRUER, Gökhan</au><au>EGÜN, Yaşar</au><au>TÜRÜTOĞLU, Hülya</au><au>CANTEKİN, Zafer</au><au>ASLANTAŞ, Özkan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Coagulase gene polymorphism of staphylococcus aurreus isolated from subclinical bovine mastitis</atitle><jtitle>Turkish journal of veterinary & animal sciences</jtitle><date>2007</date><risdate>2007</risdate><volume>31</volume><issue>4</issue><spage>253</spage><epage>257</epage><pages>253-257</pages><issn>1300-0128</issn><abstract>Özet: Bu çalışma, üç farklı şehirden (Hatay, Gaziantep ve Burdur) toplanan mastitisli sığır sütlerinden izole edilen 80 Staphylococcus
aureus susunun koagulaz gen polimorfizmini araştırmak amacıyla yapıldı. Seksen S. aureus susu 5 farklı polymerase chain reaction
(PCR) amplikonu oluştururken, 79 izolat 1 amplikon ve 1 izolat 2 amplikon oluşturdu. İzolatlar, koagulaz geninin restriction
fragment length polymorphism (RFLP) analizi ile 9 genotipe ayrıldı. İki yaygın genotip izolatların % 73,8'ini oluşturdu. Sonuçlar,
çalışılan bölgelerde farklı genotiplerin olduğunu ve bunlardan sadece bir veya iki genotipin dominant olduğunu göstermektedir
Abstract: This study was conducted to investigate the coagulase gene polymorphism of Staphylococcus aureus isolated from
subclinical bovine mastitic milk samples from 3 different Turkish cities (Hatay, Gaziantep, and Burdur). Amplification of the
coagulase gene from 80 5. aureus isolates produced 5 different polymerase chain reaction (PCR) products; 79 isolates showed only
1 amplicon, and 1 isolate showed 2 amplicons. The isolates were grouped into 9 genotypes by analysing the restriction fragment
length polymorphism (RFLP) of the gene; the 2 most common genotypes accounted for 73.8% of the isolates. The results indicated
that many coagulase gene genotypes are present in the studied regions and only 1 or 2 genotypes predominated.</abstract><pub>TÜBİTAK</pub><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1300-0128 |
| ispartof | Turkish journal of veterinary & animal sciences, 2007, Vol.31 (4), p.253-257 |
| issn | 1300-0128 |
| language | eng |
| recordid | cdi_ulakbim_primary_76377 |
| source | EZB-FREE-00999 freely available EZB journals; TÜBİTAK Scientific Journals |
| subjects | bovine mastitis cattle Dairy Animals genes genetic polymorphism Genetics and Molecular Biology of Microorganisms genetik polimorfizm genler genotip genotypes Hayvanların Prion, Viral, Bakteriyel ve Fungal Patojenleri kısıtlı fragment uzunluğu polimorfizmi mastitis meme yangısı Mikroorganizmaların Genetiği ve Moleküler Biyolojisi Prion, Viral, Bacterial and Fungal Pathogens of Animals restriction fragment length polymorphism RFLP sığır sığır meme yangısı Staphylococcus aureus subclinical mastitis subklinik meme yangısı Süt Hayvanları |
| title | Coagulase gene polymorphism of staphylococcus aurreus isolated from subclinical bovine mastitis |
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