Effects of Explant Types and Different Concentrations of Sucrose and Phytoharmones on Plant Regeneration and Hypericin Content in Hypericum perforatum L
Hypericum perforatum L. is a medicinal perennial known as "St. John's wort" in Western Europe and used in the treatment of mild to moderate depression. Hypericin, a dianthrone red pigment, is considered responsible for reversing the depression symptoms. The goal of this study was to p...
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Veröffentlicht in: | Turkish journal of agriculture and forestry 2005, Vol.29 (3), p.197-204 |
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description | Hypericum perforatum L. is a medicinal perennial known as "St. John's wort" in Western Europe and used in the treatment of mild to moderate depression. Hypericin, a dianthrone red pigment, is considered responsible for reversing the depression symptoms. The goal of this study was to produce this pigment via micropropagation and thereby describe a suitable micropropagation method for this plant. To achieve this objective, leaf discs and stem segments were cultured on Murashige and Skoog (MS) medium supplemented with kinetin and 2,4-dichlorophenoxyacetic acid (0.5, 1 and 1.5 mg l-1) and sucrose concentrations (30, 40 and 50 g l-1) in darkness at 26 ± 2 °C. The highest value was obtained from MS medium supplemented with 30 g l-1 sucrose, 0.5 mg l-1 2,4-D and 0.5 mg l-1 kinetin in terms of callus initiation frequency. In addition, callus initiation was observed in all media supplemented with different constituents. When obtained, calli were cultured on MS basal medium supplemented with 1 mg l-1 of benzyladenine for shoot induction, while the same basal medium supplemented with 1 mg l-1 of indolacetic acid was employed for rooting. The effects of factors tested on both regeneration and hypericin content were various. Number of shoots per callus and hypericin percentage were investigated and found to be higher in leaf discs originated from regenerants (19 shoots per callus and 0.048% hypericin); being easily acclimated in greenhouse conditions. |
doi_str_mv | 10.3906/tar-0403-9 |
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fullrecord | <record><control><sourceid>fao_ulakb</sourceid><recordid>TN_cdi_ulakbim_primary_50792</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>TR2005000168</sourcerecordid><originalsourceid>FETCH-LOGICAL-f1432-8e9f5fa5aa8a96afe0ab9f50fdd60514156fea8c7b0b4b52b4d420a86a096c1f3</originalsourceid><addsrcrecordid>eNpNkDFPwzAUhC0EEqWwMIOUhTHwnDiuM6JSKFIlqlLm6sWx20BiV3Yq0X_Cz8VNKsR0p7vv3fAIuaZwn-bAH1p0MTBI4_yEDGgaDKej9PSfPycX3n8CAKOQDMjPRGslWx9ZHU2-tzWaNlrut8pHaMroqQqtUyEbWyODOmwrazr6fSed9arj5pt9azfoGmvCpTXRvBtaqLUyqr_puGlYdpWszGGvPewGewx3TRRU24AHO7skZxprr66OOiQfz5PleBrP3l5ex4-zWFOWJrFQuc40ZogCc45aARYhAV2WHDLKaMa1QiFHBRSsyJKClSwBFBwh55LqdEhu-t1djV9F1ay2rmrQ7VcZjPIk1Hd9vUUvsdYOjaz8H0S5oEIIFrjbntNoV7h2gVkuEoAsfDpA6S8tkn8a</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Effects of Explant Types and Different Concentrations of Sucrose and Phytoharmones on Plant Regeneration and Hypericin Content in Hypericum perforatum L</title><source>EZB-FREE-00999 freely available EZB journals</source><source>TÜBİTAK Scientific Journals</source><creator>AYAN, Ali Kemal ; ÇIRAK, Cüneyt ; KEVSEROĞLU, Kudret ; SÖKMEN, Atalay</creator><creatorcontrib>AYAN, Ali Kemal ; ÇIRAK, Cüneyt ; KEVSEROĞLU, Kudret ; SÖKMEN, Atalay</creatorcontrib><description>Hypericum perforatum L. is a medicinal perennial known as "St. John's wort" in Western Europe and used in the treatment of mild to moderate depression. Hypericin, a dianthrone red pigment, is considered responsible for reversing the depression symptoms. The goal of this study was to produce this pigment via micropropagation and thereby describe a suitable micropropagation method for this plant. To achieve this objective, leaf discs and stem segments were cultured on Murashige and Skoog (MS) medium supplemented with kinetin and 2,4-dichlorophenoxyacetic acid (0.5, 1 and 1.5 mg l-1) and sucrose concentrations (30, 40 and 50 g l-1) in darkness at 26 ± 2 °C. The highest value was obtained from MS medium supplemented with 30 g l-1 sucrose, 0.5 mg l-1 2,4-D and 0.5 mg l-1 kinetin in terms of callus initiation frequency. In addition, callus initiation was observed in all media supplemented with different constituents. When obtained, calli were cultured on MS basal medium supplemented with 1 mg l-1 of benzyladenine for shoot induction, while the same basal medium supplemented with 1 mg l-1 of indolacetic acid was employed for rooting. The effects of factors tested on both regeneration and hypericin content were various. Number of shoots per callus and hypericin percentage were investigated and found to be higher in leaf discs originated from regenerants (19 shoots per callus and 0.048% hypericin); being easily acclimated in greenhouse conditions.</description><identifier>ISSN: 1303-6173</identifier><identifier>ISSN: 1300-011X</identifier><identifier>EISSN: 1303-6173</identifier><identifier>DOI: 10.3906/tar-0403-9</identifier><language>eng</language><publisher>Ankara: TUBITAK</publisher><subject>2,4-D ; 2,4-D-2,4-dichlorophenoxyacetic acid ; Agronomy. Soil science and plant productions ; Bahçe Bitkileri Ürünleri ; benziladenin ; benzyladenine ; Biological and medical sciences ; Biotechnology ; Bitki Bileşimi ; bitki büyüme düzenleyicileri ; Bitki Fizyolojisi ve Biyokimyası ; Bitki Materyallerinin İn Vitro Kültürü ; Bitki Çoğaltımı ; Bitki Üretimi ; callus ; chemical composition ; culture media ; doku kültürü ; Fundamental and applied biological sciences. Psychology ; General agronomy. Plant production ; Gıda ve Yem Olmayan Bitkisel Ürünler ; gövde ; Horticultural Crops ; Hypericum perforatum ; Hypericum perforatum L., micropropagation, hypericin. Abbreviations: BA-6-benzyladenine ; IAA ; IAA-indolacetic acid ; IBA-indolebutyric acid ; in vitro culture ; in vitro Culture of Plant Material ; in vitro kültür ; in vitro regeneration ; in vitro yenilenme ; kallus ; kimyasal bileşim ; kinetin ; köklenme ; kültür ortamı ; leaves ; medicinal plants ; micropropagation ; mikroçoğaltma ; NAA-a-naphthaleneacetic acid ; Non-food/Non-feed Plant Products ; Plant Composition ; plant growth regulators ; Plant Physiology and Biochemistry ; Plant Production ; Plant Propagation ; rooting ; sakaroz ; stems ; sucrose ; tıbbi bitkiler ; tissue culture ; Vegetative propagation. Micropropagation ; Vegetative propagation. Sowing and planting. Harvesting ; yapraklar</subject><ispartof>Turkish journal of agriculture and forestry, 2005, Vol.29 (3), p.197-204</ispartof><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16818884$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>AYAN, Ali Kemal</creatorcontrib><creatorcontrib>ÇIRAK, Cüneyt</creatorcontrib><creatorcontrib>KEVSEROĞLU, Kudret</creatorcontrib><creatorcontrib>SÖKMEN, Atalay</creatorcontrib><title>Effects of Explant Types and Different Concentrations of Sucrose and Phytoharmones on Plant Regeneration and Hypericin Content in Hypericum perforatum L</title><title>Turkish journal of agriculture and forestry</title><description>Hypericum perforatum L. is a medicinal perennial known as "St. John's wort" in Western Europe and used in the treatment of mild to moderate depression. Hypericin, a dianthrone red pigment, is considered responsible for reversing the depression symptoms. The goal of this study was to produce this pigment via micropropagation and thereby describe a suitable micropropagation method for this plant. To achieve this objective, leaf discs and stem segments were cultured on Murashige and Skoog (MS) medium supplemented with kinetin and 2,4-dichlorophenoxyacetic acid (0.5, 1 and 1.5 mg l-1) and sucrose concentrations (30, 40 and 50 g l-1) in darkness at 26 ± 2 °C. The highest value was obtained from MS medium supplemented with 30 g l-1 sucrose, 0.5 mg l-1 2,4-D and 0.5 mg l-1 kinetin in terms of callus initiation frequency. In addition, callus initiation was observed in all media supplemented with different constituents. When obtained, calli were cultured on MS basal medium supplemented with 1 mg l-1 of benzyladenine for shoot induction, while the same basal medium supplemented with 1 mg l-1 of indolacetic acid was employed for rooting. The effects of factors tested on both regeneration and hypericin content were various. Number of shoots per callus and hypericin percentage were investigated and found to be higher in leaf discs originated from regenerants (19 shoots per callus and 0.048% hypericin); being easily acclimated in greenhouse conditions.</description><subject>2,4-D</subject><subject>2,4-D-2,4-dichlorophenoxyacetic acid</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Bahçe Bitkileri Ürünleri</subject><subject>benziladenin</subject><subject>benzyladenine</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Bitki Bileşimi</subject><subject>bitki büyüme düzenleyicileri</subject><subject>Bitki Fizyolojisi ve Biyokimyası</subject><subject>Bitki Materyallerinin İn Vitro Kültürü</subject><subject>Bitki Çoğaltımı</subject><subject>Bitki Üretimi</subject><subject>callus</subject><subject>chemical composition</subject><subject>culture media</subject><subject>doku kültürü</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General agronomy. Plant production</subject><subject>Gıda ve Yem Olmayan Bitkisel Ürünler</subject><subject>gövde</subject><subject>Horticultural Crops</subject><subject>Hypericum perforatum</subject><subject>Hypericum perforatum L., micropropagation, hypericin. Abbreviations: BA-6-benzyladenine</subject><subject>IAA</subject><subject>IAA-indolacetic acid</subject><subject>IBA-indolebutyric acid</subject><subject>in vitro culture</subject><subject>in vitro Culture of Plant Material</subject><subject>in vitro kültür</subject><subject>in vitro regeneration</subject><subject>in vitro yenilenme</subject><subject>kallus</subject><subject>kimyasal bileşim</subject><subject>kinetin</subject><subject>köklenme</subject><subject>kültür ortamı</subject><subject>leaves</subject><subject>medicinal plants</subject><subject>micropropagation</subject><subject>mikroçoğaltma</subject><subject>NAA-a-naphthaleneacetic acid</subject><subject>Non-food/Non-feed Plant Products</subject><subject>Plant Composition</subject><subject>plant growth regulators</subject><subject>Plant Physiology and Biochemistry</subject><subject>Plant Production</subject><subject>Plant Propagation</subject><subject>rooting</subject><subject>sakaroz</subject><subject>stems</subject><subject>sucrose</subject><subject>tıbbi bitkiler</subject><subject>tissue culture</subject><subject>Vegetative propagation. Micropropagation</subject><subject>Vegetative propagation. Sowing and planting. Harvesting</subject><subject>yapraklar</subject><issn>1303-6173</issn><issn>1300-011X</issn><issn>1303-6173</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNpNkDFPwzAUhC0EEqWwMIOUhTHwnDiuM6JSKFIlqlLm6sWx20BiV3Yq0X_Cz8VNKsR0p7vv3fAIuaZwn-bAH1p0MTBI4_yEDGgaDKej9PSfPycX3n8CAKOQDMjPRGslWx9ZHU2-tzWaNlrut8pHaMroqQqtUyEbWyODOmwrazr6fSed9arj5pt9azfoGmvCpTXRvBtaqLUyqr_puGlYdpWszGGvPewGewx3TRRU24AHO7skZxprr66OOiQfz5PleBrP3l5ex4-zWFOWJrFQuc40ZogCc45aARYhAV2WHDLKaMa1QiFHBRSsyJKClSwBFBwh55LqdEhu-t1djV9F1ay2rmrQ7VcZjPIk1Hd9vUUvsdYOjaz8H0S5oEIIFrjbntNoV7h2gVkuEoAsfDpA6S8tkn8a</recordid><startdate>2005</startdate><enddate>2005</enddate><creator>AYAN, Ali Kemal</creator><creator>ÇIRAK, Cüneyt</creator><creator>KEVSEROĞLU, Kudret</creator><creator>SÖKMEN, Atalay</creator><general>TUBITAK</general><general>Tubitak</general><general>TÜBİTAK</general><scope>FBQ</scope><scope>IQODW</scope></search><sort><creationdate>2005</creationdate><title>Effects of Explant Types and Different Concentrations of Sucrose and Phytoharmones on Plant Regeneration and Hypericin Content in Hypericum perforatum L</title><author>AYAN, Ali Kemal ; ÇIRAK, Cüneyt ; KEVSEROĞLU, Kudret ; SÖKMEN, Atalay</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f1432-8e9f5fa5aa8a96afe0ab9f50fdd60514156fea8c7b0b4b52b4d420a86a096c1f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>2,4-D</topic><topic>2,4-D-2,4-dichlorophenoxyacetic acid</topic><topic>Agronomy. Soil science and plant productions</topic><topic>Bahçe Bitkileri Ürünleri</topic><topic>benziladenin</topic><topic>benzyladenine</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Bitki Bileşimi</topic><topic>bitki büyüme düzenleyicileri</topic><topic>Bitki Fizyolojisi ve Biyokimyası</topic><topic>Bitki Materyallerinin İn Vitro Kültürü</topic><topic>Bitki Çoğaltımı</topic><topic>Bitki Üretimi</topic><topic>callus</topic><topic>chemical composition</topic><topic>culture media</topic><topic>doku kültürü</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General agronomy. Plant production</topic><topic>Gıda ve Yem Olmayan Bitkisel Ürünler</topic><topic>gövde</topic><topic>Horticultural Crops</topic><topic>Hypericum perforatum</topic><topic>Hypericum perforatum L., micropropagation, hypericin. Abbreviations: BA-6-benzyladenine</topic><topic>IAA</topic><topic>IAA-indolacetic acid</topic><topic>IBA-indolebutyric acid</topic><topic>in vitro culture</topic><topic>in vitro Culture of Plant Material</topic><topic>in vitro kültür</topic><topic>in vitro regeneration</topic><topic>in vitro yenilenme</topic><topic>kallus</topic><topic>kimyasal bileşim</topic><topic>kinetin</topic><topic>köklenme</topic><topic>kültür ortamı</topic><topic>leaves</topic><topic>medicinal plants</topic><topic>micropropagation</topic><topic>mikroçoğaltma</topic><topic>NAA-a-naphthaleneacetic acid</topic><topic>Non-food/Non-feed Plant Products</topic><topic>Plant Composition</topic><topic>plant growth regulators</topic><topic>Plant Physiology and Biochemistry</topic><topic>Plant Production</topic><topic>Plant Propagation</topic><topic>rooting</topic><topic>sakaroz</topic><topic>stems</topic><topic>sucrose</topic><topic>tıbbi bitkiler</topic><topic>tissue culture</topic><topic>Vegetative propagation. Micropropagation</topic><topic>Vegetative propagation. Sowing and planting. Harvesting</topic><topic>yapraklar</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>AYAN, Ali Kemal</creatorcontrib><creatorcontrib>ÇIRAK, Cüneyt</creatorcontrib><creatorcontrib>KEVSEROĞLU, Kudret</creatorcontrib><creatorcontrib>SÖKMEN, Atalay</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><jtitle>Turkish journal of agriculture and forestry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>AYAN, Ali Kemal</au><au>ÇIRAK, Cüneyt</au><au>KEVSEROĞLU, Kudret</au><au>SÖKMEN, Atalay</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Explant Types and Different Concentrations of Sucrose and Phytoharmones on Plant Regeneration and Hypericin Content in Hypericum perforatum L</atitle><jtitle>Turkish journal of agriculture and forestry</jtitle><date>2005</date><risdate>2005</risdate><volume>29</volume><issue>3</issue><spage>197</spage><epage>204</epage><pages>197-204</pages><issn>1303-6173</issn><issn>1300-011X</issn><eissn>1303-6173</eissn><abstract>Hypericum perforatum L. is a medicinal perennial known as "St. John's wort" in Western Europe and used in the treatment of mild to moderate depression. Hypericin, a dianthrone red pigment, is considered responsible for reversing the depression symptoms. The goal of this study was to produce this pigment via micropropagation and thereby describe a suitable micropropagation method for this plant. To achieve this objective, leaf discs and stem segments were cultured on Murashige and Skoog (MS) medium supplemented with kinetin and 2,4-dichlorophenoxyacetic acid (0.5, 1 and 1.5 mg l-1) and sucrose concentrations (30, 40 and 50 g l-1) in darkness at 26 ± 2 °C. The highest value was obtained from MS medium supplemented with 30 g l-1 sucrose, 0.5 mg l-1 2,4-D and 0.5 mg l-1 kinetin in terms of callus initiation frequency. In addition, callus initiation was observed in all media supplemented with different constituents. When obtained, calli were cultured on MS basal medium supplemented with 1 mg l-1 of benzyladenine for shoot induction, while the same basal medium supplemented with 1 mg l-1 of indolacetic acid was employed for rooting. The effects of factors tested on both regeneration and hypericin content were various. Number of shoots per callus and hypericin percentage were investigated and found to be higher in leaf discs originated from regenerants (19 shoots per callus and 0.048% hypericin); being easily acclimated in greenhouse conditions.</abstract><cop>Ankara</cop><pub>TUBITAK</pub><doi>10.3906/tar-0403-9</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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source | EZB-FREE-00999 freely available EZB journals; TÜBİTAK Scientific Journals |
subjects | 2,4-D 2,4-D-2,4-dichlorophenoxyacetic acid Agronomy. Soil science and plant productions Bahçe Bitkileri Ürünleri benziladenin benzyladenine Biological and medical sciences Biotechnology Bitki Bileşimi bitki büyüme düzenleyicileri Bitki Fizyolojisi ve Biyokimyası Bitki Materyallerinin İn Vitro Kültürü Bitki Çoğaltımı Bitki Üretimi callus chemical composition culture media doku kültürü Fundamental and applied biological sciences. Psychology General agronomy. Plant production Gıda ve Yem Olmayan Bitkisel Ürünler gövde Horticultural Crops Hypericum perforatum Hypericum perforatum L., micropropagation, hypericin. Abbreviations: BA-6-benzyladenine IAA IAA-indolacetic acid IBA-indolebutyric acid in vitro culture in vitro Culture of Plant Material in vitro kültür in vitro regeneration in vitro yenilenme kallus kimyasal bileşim kinetin köklenme kültür ortamı leaves medicinal plants micropropagation mikroçoğaltma NAA-a-naphthaleneacetic acid Non-food/Non-feed Plant Products Plant Composition plant growth regulators Plant Physiology and Biochemistry Plant Production Plant Propagation rooting sakaroz stems sucrose tıbbi bitkiler tissue culture Vegetative propagation. Micropropagation Vegetative propagation. Sowing and planting. Harvesting yapraklar |
title | Effects of Explant Types and Different Concentrations of Sucrose and Phytoharmones on Plant Regeneration and Hypericin Content in Hypericum perforatum L |
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