Modeling of TaqI endonuclease purification by HPLC
The present work is based on the previously reported purification study of TaqI $endonuclease^5$ and aimed to simulate the experimentally obtained chromatograms in order to reduce the time and cost in the design, optimization and scaling up of purification processes. Three different models depending...
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| Veröffentlicht in: | Turkish journal of chemistry 2002, Vol.26 (6), p.877-887 |
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| container_title | Turkish journal of chemistry |
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| creator | ÜLGEN, Kutlu Ö DÜZENLİ, Mehmet |
| description | The present work is based on the previously reported purification study of TaqI $endonuclease^5$ and aimed to simulate the experimentally obtained chromatograms in order to reduce the time and cost in the design, optimization and scaling up of purification processes. Three different models depending on equilibrium, rate and plate theories are used in the mathematical description of experimental elution profiles and the solutions of the model equations are compared. The equilibrium-dispersive theory provides excellent results in predicting the experimental chromatograms.It is thus recognized that band dispersion takes place in the column through axial dispersion and nonequilibrium effects. The user-friendly software written in Visual Basic can be used in the design of purification processes as well as in the determination of optimal separation conditions for many other enzymes. |
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Three different models depending on equilibrium, rate and plate theories are used in the mathematical description of experimental elution profiles and the solutions of the model equations are compared. The equilibrium-dispersive theory provides excellent results in predicting the experimental chromatograms.It is thus recognized that band dispersion takes place in the column through axial dispersion and nonequilibrium effects. 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Three different models depending on equilibrium, rate and plate theories are used in the mathematical description of experimental elution profiles and the solutions of the model equations are compared. The equilibrium-dispersive theory provides excellent results in predicting the experimental chromatograms.It is thus recognized that band dispersion takes place in the column through axial dispersion and nonequilibrium effects. The user-friendly software written in Visual Basic can be used in the design of purification processes as well as in the determination of optimal separation conditions for many other enzymes.</description><subject>Analitik Kimya, Ayırma Yöntemleri</subject><subject>Analytical Chemistry, Separation Methods</subject><subject>Ayırma</subject><subject>Biochemistry</subject><subject>Biyokimya</subject><subject>chromatography</subject><subject>Denge</subject><subject>Denge-dağıtıcı kuramı</subject><subject>design</subject><subject>endonuclease</subject><subject>Endonükleaz</subject><subject>equilibrium</subject><subject>Equilibrium-dispersive theory</subject><subject>Kromatografi</subject><subject>modeling</subject><subject>Modelleme</subject><subject>Plak kuramı</subject><subject>Plate theory</subject><subject>purification</subject><subject>Saflaştırma</subject><subject>separation</subject><subject>Taq I enzimi</subject><subject>Taq I enzyme</subject><subject>Tasarım</subject><issn>1300-0527</issn><issn>1303-6130</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNotjMFKAzEURYNYsLb-gYv8QOAlmcwkSxnUFkZ0UdflJXmR6HSmTjqL_r1Fhcs5Z3Wv2FJq0KK-8Pq3QYBRzQ27LeUTQLrLlky9jJH6PHzwMfEdfm85DXEc5tATFuLHecopBzzlceD-zDdvXbtmi4R9obt_r9j70-Ou3Yju9XnbPnSiyKY6iWiMU9YlkEG62lP0gAgklY5VYzFVibxDq0O0KVhFJijAoImiQ21k0Ct2__c79_jl82F_nPIBp_NeK2dr_QNxeD9M</recordid><startdate>2002</startdate><enddate>2002</enddate><creator>ÜLGEN, Kutlu Ö</creator><creator>DÜZENLİ, Mehmet</creator><general>TÜBİTAK</general><scope/></search><sort><creationdate>2002</creationdate><title>Modeling of TaqI endonuclease purification by HPLC</title><author>ÜLGEN, Kutlu Ö ; DÜZENLİ, Mehmet</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-s174t-d559289f01c196bedb0aa0e123d478af4feb9a83cd8fc82e5c20ac3eed9a351c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Analitik Kimya, Ayırma Yöntemleri</topic><topic>Analytical Chemistry, Separation Methods</topic><topic>Ayırma</topic><topic>Biochemistry</topic><topic>Biyokimya</topic><topic>chromatography</topic><topic>Denge</topic><topic>Denge-dağıtıcı kuramı</topic><topic>design</topic><topic>endonuclease</topic><topic>Endonükleaz</topic><topic>equilibrium</topic><topic>Equilibrium-dispersive theory</topic><topic>Kromatografi</topic><topic>modeling</topic><topic>Modelleme</topic><topic>Plak kuramı</topic><topic>Plate theory</topic><topic>purification</topic><topic>Saflaştırma</topic><topic>separation</topic><topic>Taq I enzimi</topic><topic>Taq I enzyme</topic><topic>Tasarım</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ÜLGEN, Kutlu Ö</creatorcontrib><creatorcontrib>DÜZENLİ, Mehmet</creatorcontrib><jtitle>Turkish journal of chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ÜLGEN, Kutlu Ö</au><au>DÜZENLİ, Mehmet</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modeling of TaqI endonuclease purification by HPLC</atitle><jtitle>Turkish journal of chemistry</jtitle><date>2002</date><risdate>2002</risdate><volume>26</volume><issue>6</issue><spage>877</spage><epage>887</epage><pages>877-887</pages><issn>1300-0527</issn><eissn>1303-6130</eissn><abstract>The present work is based on the previously reported purification study of TaqI $endonuclease^5$ and aimed to simulate the experimentally obtained chromatograms in order to reduce the time and cost in the design, optimization and scaling up of purification processes. Three different models depending on equilibrium, rate and plate theories are used in the mathematical description of experimental elution profiles and the solutions of the model equations are compared. The equilibrium-dispersive theory provides excellent results in predicting the experimental chromatograms.It is thus recognized that band dispersion takes place in the column through axial dispersion and nonequilibrium effects. The user-friendly software written in Visual Basic can be used in the design of purification processes as well as in the determination of optimal separation conditions for many other enzymes.</abstract><pub>TÜBİTAK</pub><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1300-0527 |
| ispartof | Turkish journal of chemistry, 2002, Vol.26 (6), p.877-887 |
| issn | 1300-0527 1303-6130 |
| language | eng |
| recordid | cdi_ulakbim_primary_32986 |
| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; TÜBİTAK Scientific Journals |
| subjects | Analitik Kimya, Ayırma Yöntemleri Analytical Chemistry, Separation Methods Ayırma Biochemistry Biyokimya chromatography Denge Denge-dağıtıcı kuramı design endonuclease Endonükleaz equilibrium Equilibrium-dispersive theory Kromatografi modeling Modelleme Plak kuramı Plate theory purification Saflaştırma separation Taq I enzimi Taq I enzyme Tasarım |
| title | Modeling of TaqI endonuclease purification by HPLC |
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