Molecular cloning of an alpha amylase gene from Bacillus subtilis RSKK246 and its expression in Escherichia coli and in Bacillus subtilis

Bacillus subtilis RSKK246 was found to produce approximately a 65-kDa $\alpha$-amylase enzyme. A gene was isolated encoding $\alpha$-amylase activity that corresponded to this size and was inserted into pUC18 plasmid which was transferred to Escherichia coli. An approximately 1.7kbp fragment, which...

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Veröffentlicht in:	Turkish journal of veterinary & animal sciences 2001, Vol.25 (2), p.197-201
Hauptverfasser:	ALTINALAN, Adem, ÖZCAN, Numan
Format:	Artikel
Sprache:	eng
Schlagworte:	alfa-amilaz alpha-amylase Bacillus subtilis Biotechnology Biyoteknoloji DNA cloning DNA klonlama Escherichia coli gen ifadesi gene expression Genetics and Molecular Biology of Microorganisms Mikroorganizmaların Genetiği ve Moleküler Biyolojisi
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