Screening of thalassemia in Hong Kong Chinese by HPLC method
The prevalence of $\alpha$-thalassemia and HbH disease are high in Hong Kong Chinese. The Bio-Rad VARIANT, a cation exchange HPLC method for HbA2, HbF and certain haemoglobin variants determination provide a rapid screening for thalassemia. In this study, the reference ranges of HbA2 and HbF in Hong...
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| Veröffentlicht in: | Turkish journal of haematology 2001, Vol.18 (1), p.27-31 |
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| creator | YOW, C. M. N TSE, Y. K SZETO, Y. T |
| description | The prevalence of $\alpha$-thalassemia and HbH disease are high in Hong Kong Chinese. The Bio-Rad VARIANT, a cation exchange HPLC method for HbA2, HbF and certain haemoglobin variants determination provide a rapid screening for thalassemia. In this study, the reference ranges of HbA2 and HbF in Hong Kong Chinese were first established for this analyser. It was 2.2-3.0% and 0-1.0% respectively which are similar to Caucasians. In addition, in agreement with the manufacturer's pilot study, the system was confirmed to be able to detect Hb-H. We have demonstrated a peak at 0.5 min observed in the chromatogram of all 30 HbH disease specimens. To conclude, HPLC method shows a similar reference range of HbA2 and HbF between the Hong Kong Chinese and the Caucasians. It also provides a rapid diagnosis of HbH disease which is common in south-east Asia. Manual determination of HbA2 using Bio-Rad Microcolumn HbA2 kit and Betke's manual method for HbF were comparable with the Bio-Rad VARIANT. |
| format | Article |
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K ; SZETO, Y. T</creatorcontrib><description>The prevalence of $\alpha$-thalassemia and HbH disease are high in Hong Kong Chinese. The Bio-Rad VARIANT, a cation exchange HPLC method for HbA2, HbF and certain haemoglobin variants determination provide a rapid screening for thalassemia. In this study, the reference ranges of HbA2 and HbF in Hong Kong Chinese were first established for this analyser. It was 2.2-3.0% and 0-1.0% respectively which are similar to Caucasians. In addition, in agreement with the manufacturer's pilot study, the system was confirmed to be able to detect Hb-H. We have demonstrated a peak at 0.5 min observed in the chromatogram of all 30 HbH disease specimens. To conclude, HPLC method shows a similar reference range of HbA2 and HbF between the Hong Kong Chinese and the Caucasians. It also provides a rapid diagnosis of HbH disease which is common in south-east Asia. 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M. N</creatorcontrib><creatorcontrib>TSE, Y. K</creatorcontrib><creatorcontrib>SZETO, Y. T</creatorcontrib><title>Screening of thalassemia in Hong Kong Chinese by HPLC method</title><title>Turkish journal of haematology</title><description>The prevalence of $\alpha$-thalassemia and HbH disease are high in Hong Kong Chinese. The Bio-Rad VARIANT, a cation exchange HPLC method for HbA2, HbF and certain haemoglobin variants determination provide a rapid screening for thalassemia. In this study, the reference ranges of HbA2 and HbF in Hong Kong Chinese were first established for this analyser. It was 2.2-3.0% and 0-1.0% respectively which are similar to Caucasians. In addition, in agreement with the manufacturer's pilot study, the system was confirmed to be able to detect Hb-H. We have demonstrated a peak at 0.5 min observed in the chromatogram of all 30 HbH disease specimens. To conclude, HPLC method shows a similar reference range of HbA2 and HbF between the Hong Kong Chinese and the Caucasians. It also provides a rapid diagnosis of HbH disease which is common in south-east Asia. Manual determination of HbA2 using Bio-Rad Microcolumn HbA2 kit and Betke's manual method for HbF were comparable with the Bio-Rad VARIANT.</description><subject>alfa-Talasemi</subject><subject>alpha-Thalassemia</subject><subject>Amino acids, peptides and proteins</subject><subject>Aminoasitler, peptidler ve proteinler</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Fetal hemoglobin</subject><subject>Hemic and lymphatic diseases</subject><subject>Hemik ve lenfatik hastalıklar</subject><subject>Hemoglobin A2</subject><subject>Hemoglobin H</subject><subject>Kromatografi, yüksek basınçlı sıvı</subject><subject>Referans değerleri</subject><subject>Reference Values</subject><issn>1300-7777</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNotjL1qwzAURjUkEDfNG3TQCxiuJEu2IEswbV1iaKHZw5V1XSvxT7DSIW_flPYbzoEzfAuWCAWQ5vet2EOMJwBZGAkJ2342M9EYxi8-tfzaYY8x0hCQh5FX0z3vf1F2YaRI3N149VGXfKBrN_lHtmyxj7T595odXp4PZZXW769v5a5OY2FFShmQy3JLuVGgtTVGK2uczq1sGmy99NTKzDvyDjSizRSQ11oJdM4IJdSaPf3dfvd4dmE4XuYw4Hw7CmOkUD8OcD9K</recordid><startdate>2001</startdate><enddate>2001</enddate><creator>YOW, C. M. N</creator><creator>TSE, Y. K</creator><creator>SZETO, Y. T</creator><general>Türk Hematoloji Derneği</general><scope/></search><sort><creationdate>2001</creationdate><title>Screening of thalassemia in Hong Kong Chinese by HPLC method</title><author>YOW, C. M. N ; TSE, Y. K ; SZETO, Y. T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-s891-e40eb479e7630559665396b5792ccafd2def24dbedb05aa9430ed5531abb61313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>alfa-Talasemi</topic><topic>alpha-Thalassemia</topic><topic>Amino acids, peptides and proteins</topic><topic>Aminoasitler, peptidler ve proteinler</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Fetal hemoglobin</topic><topic>Hemic and lymphatic diseases</topic><topic>Hemik ve lenfatik hastalıklar</topic><topic>Hemoglobin A2</topic><topic>Hemoglobin H</topic><topic>Kromatografi, yüksek basınçlı sıvı</topic><topic>Referans değerleri</topic><topic>Reference Values</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>YOW, C. M. N</creatorcontrib><creatorcontrib>TSE, Y. K</creatorcontrib><creatorcontrib>SZETO, Y. T</creatorcontrib><jtitle>Turkish journal of haematology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>YOW, C. M. N</au><au>TSE, Y. K</au><au>SZETO, Y. T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Screening of thalassemia in Hong Kong Chinese by HPLC method</atitle><jtitle>Turkish journal of haematology</jtitle><date>2001</date><risdate>2001</risdate><volume>18</volume><issue>1</issue><spage>27</spage><epage>31</epage><pages>27-31</pages><issn>1300-7777</issn><abstract>The prevalence of $\alpha$-thalassemia and HbH disease are high in Hong Kong Chinese. The Bio-Rad VARIANT, a cation exchange HPLC method for HbA2, HbF and certain haemoglobin variants determination provide a rapid screening for thalassemia. In this study, the reference ranges of HbA2 and HbF in Hong Kong Chinese were first established for this analyser. It was 2.2-3.0% and 0-1.0% respectively which are similar to Caucasians. In addition, in agreement with the manufacturer's pilot study, the system was confirmed to be able to detect Hb-H. We have demonstrated a peak at 0.5 min observed in the chromatogram of all 30 HbH disease specimens. To conclude, HPLC method shows a similar reference range of HbA2 and HbF between the Hong Kong Chinese and the Caucasians. It also provides a rapid diagnosis of HbH disease which is common in south-east Asia. Manual determination of HbA2 using Bio-Rad Microcolumn HbA2 kit and Betke's manual method for HbF were comparable with the Bio-Rad VARIANT.</abstract><pub>Türk Hematoloji Derneği</pub><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1300-7777 |
| ispartof | Turkish journal of haematology, 2001, Vol.18 (1), p.27-31 |
| issn | 1300-7777 |
| language | eng |
| recordid | cdi_ulakbim_primary_16621 |
| source | DOAJ Directory of Open Access Journals |
| subjects | alfa-Talasemi alpha-Thalassemia Amino acids, peptides and proteins Aminoasitler, peptidler ve proteinler Chromatography, High Pressure Liquid Fetal hemoglobin Hemic and lymphatic diseases Hemik ve lenfatik hastalıklar Hemoglobin A2 Hemoglobin H Kromatografi, yüksek basınçlı sıvı Referans değerleri Reference Values |
| title | Screening of thalassemia in Hong Kong Chinese by HPLC method |
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