Effects of hypertension and ovariectomy on rat hepatocytes. Are amlodipine and lacidipine protective? (A stereological and histological study)
Calcium channel blockers are increasingly used for the treatment of hypertension. Menopause and hypertension are both important risk factors for liver damage and several other circulatory abnormalities. The aim of this study was to determine the effects of amlodipine and lacidipine in an ovariectomy...
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| Veröffentlicht in: | The Turkish journal of gastroenterology 2010-12, Vol.21 (4), p.387-395 |
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| container_title | The Turkish journal of gastroenterology |
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| creator | Dursun, Hakan Albayrak, Fatih Uyanik, Abdullah Keleş, Nuri Osman Beyzagül, Polat Bayram, Ednan Halici, Zekai Altunkaynak, Zuhal Berrin Süleyman, Halis Okçu, Nihat Ünal, Bünyamin |
| description | Calcium channel blockers are increasingly used for the treatment of hypertension. Menopause and hypertension are both important risk factors for liver damage and several other circulatory abnormalities. The aim of this study was to determine the effects of amlodipine and lacidipine in an ovariectomy-induced postmenopausal period model and a deoxycorticosterone acetate-salt-induced hypertensive model in rats.
In this study, animals were divided into six groups as follows: control (Group 1), hypertension (Group 2), ovariectomy (Group 3), ovariectomy and hypertension (Group 4), ovariectomy, hypertension and amlodipine-treated (Group 5), and ovariectomy, hypertension and lacidipine-treated (Group 6). At the end of the experiment, the livers were removed and tissue samples were histologically and stereologically examined.
The numerical densities of the hepatocytes according to group were 0.000422, 0.00329, 0.000272, 0.00259, 0.00374 and 0.000346 μm3, respectively. Significant differences were found between values of all groups (p |
| doi_str_mv | 10.4318/tjg.2010.0125 |
| format | Article |
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In this study, animals were divided into six groups as follows: control (Group 1), hypertension (Group 2), ovariectomy (Group 3), ovariectomy and hypertension (Group 4), ovariectomy, hypertension and amlodipine-treated (Group 5), and ovariectomy, hypertension and lacidipine-treated (Group 6). At the end of the experiment, the livers were removed and tissue samples were histologically and stereologically examined.
The numerical densities of the hepatocytes according to group were 0.000422, 0.00329, 0.000272, 0.00259, 0.00374 and 0.000346 μm3, respectively. Significant differences were found between values of all groups (p<0.01, Mann-Whitney U test). According to histopathological investigation, Group 3 and particularly Group 4 showed some microscopic abnormalities such as dilatation in sinusoids central veins and branches of portal vein, irregularities of the hepatocyte columns, significant mononuclear cell infiltrations, and unstained vacuoles in the cytoplasm of the hepatocytes. Histological structure was protected from the destructive effects of ovariectomy and hypertension in Groups 5 and 6.
Our experimental results show that both hypertension and the postmenopausal period have negative effects on the number of hepatocytes and histological structure of the liver. Both amlodipine and lacidipine appear to ameliorate the hypertension and/or postmenopausal period-related decrease in hepatocyte number. We thus suggest that lacidipine and particularly amlodipine have important protective and recovering effects on the liver.</description><identifier>ISSN: 1300-4948</identifier><identifier>EISSN: 2148-5607</identifier><identifier>DOI: 10.4318/tjg.2010.0125</identifier><identifier>PMID: 21331992</identifier><language>eng</language><publisher>Turkey: Türk Gastroenteroloji Vakfı</publisher><subject>Amlodipine - pharmacology ; Animals ; Calcium Channel Blockers - pharmacology ; Cytoprotection - drug effects ; Dihydropyridines - pharmacology ; Female ; Hepatocytes - drug effects ; Hepatocytes - pathology ; Hypertension - drug therapy ; Liver - drug effects ; Liver - pathology ; Ovariectomy ; Postmenopause ; Rats ; Rats, Inbred Strains</subject><ispartof>The Turkish journal of gastroenterology, 2010-12, Vol.21 (4), p.387-395</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c314t-79fe1bc5095f04c3b0a01674f6710e9f1d122268ad1c17f610aef436c0d8e7013</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21331992$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dursun, Hakan</creatorcontrib><creatorcontrib>Albayrak, Fatih</creatorcontrib><creatorcontrib>Uyanik, Abdullah</creatorcontrib><creatorcontrib>Keleş, Nuri Osman</creatorcontrib><creatorcontrib>Beyzagül, Polat</creatorcontrib><creatorcontrib>Bayram, Ednan</creatorcontrib><creatorcontrib>Halici, Zekai</creatorcontrib><creatorcontrib>Altunkaynak, Zuhal Berrin</creatorcontrib><creatorcontrib>Süleyman, Halis</creatorcontrib><creatorcontrib>Okçu, Nihat</creatorcontrib><creatorcontrib>Ünal, Bünyamin</creatorcontrib><title>Effects of hypertension and ovariectomy on rat hepatocytes. Are amlodipine and lacidipine protective? (A stereological and histological study)</title><title>The Turkish journal of gastroenterology</title><addtitle>Turk J Gastroenterol</addtitle><description>Calcium channel blockers are increasingly used for the treatment of hypertension. Menopause and hypertension are both important risk factors for liver damage and several other circulatory abnormalities. The aim of this study was to determine the effects of amlodipine and lacidipine in an ovariectomy-induced postmenopausal period model and a deoxycorticosterone acetate-salt-induced hypertensive model in rats.
In this study, animals were divided into six groups as follows: control (Group 1), hypertension (Group 2), ovariectomy (Group 3), ovariectomy and hypertension (Group 4), ovariectomy, hypertension and amlodipine-treated (Group 5), and ovariectomy, hypertension and lacidipine-treated (Group 6). At the end of the experiment, the livers were removed and tissue samples were histologically and stereologically examined.
The numerical densities of the hepatocytes according to group were 0.000422, 0.00329, 0.000272, 0.00259, 0.00374 and 0.000346 μm3, respectively. Significant differences were found between values of all groups (p<0.01, Mann-Whitney U test). According to histopathological investigation, Group 3 and particularly Group 4 showed some microscopic abnormalities such as dilatation in sinusoids central veins and branches of portal vein, irregularities of the hepatocyte columns, significant mononuclear cell infiltrations, and unstained vacuoles in the cytoplasm of the hepatocytes. Histological structure was protected from the destructive effects of ovariectomy and hypertension in Groups 5 and 6.
Our experimental results show that both hypertension and the postmenopausal period have negative effects on the number of hepatocytes and histological structure of the liver. Both amlodipine and lacidipine appear to ameliorate the hypertension and/or postmenopausal period-related decrease in hepatocyte number. We thus suggest that lacidipine and particularly amlodipine have important protective and recovering effects on the liver.</description><subject>Amlodipine - pharmacology</subject><subject>Animals</subject><subject>Calcium Channel Blockers - pharmacology</subject><subject>Cytoprotection - drug effects</subject><subject>Dihydropyridines - pharmacology</subject><subject>Female</subject><subject>Hepatocytes - drug effects</subject><subject>Hepatocytes - pathology</subject><subject>Hypertension - drug therapy</subject><subject>Liver - drug effects</subject><subject>Liver - pathology</subject><subject>Ovariectomy</subject><subject>Postmenopause</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><issn>1300-4948</issn><issn>2148-5607</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkU1P3DAQhq2Kqmxpjz2CfKM9ZDtj5_OEVoh-SEi9tOfI64xZQxIH20HKn-hvrpdd6Gk0877zSjMPY58Q1rnE-mu8v1sLSB2gKN6wlcC8zooSqhO2QgmQ5U1en7L3IdwDyBpL8Y6dCpQSm0as2N8bY0jHwJ3hu2UiH2kM1o1cjR13T8rbpLph4WnkVeQ7mlR0eokU1nzjiauhd52d7EjPK73S9thO3sW0bJ_oin_e8BDJk-vdndWqf_bubIivgxDnbvnygb01qg_08VjP2J9vN7-vf2S3v77_vN7cZlpiHrOqMYRbXUBTGMi13IICLKvclBUCNQY7FEKUtepQY2VKBEUml6WGrqYKUJ6x80Pu3KuHrR3aydtB-aVFIYUskn550NMRjzOF2A42aOp7NZKbQ1sXokk_bKrkzA5O7V0Insz_KGj3hNpEqN0TaveEkv_imDxvB-pe3S9I5D-csI29</recordid><startdate>20101201</startdate><enddate>20101201</enddate><creator>Dursun, Hakan</creator><creator>Albayrak, Fatih</creator><creator>Uyanik, Abdullah</creator><creator>Keleş, Nuri Osman</creator><creator>Beyzagül, Polat</creator><creator>Bayram, Ednan</creator><creator>Halici, Zekai</creator><creator>Altunkaynak, Zuhal Berrin</creator><creator>Süleyman, Halis</creator><creator>Okçu, Nihat</creator><creator>Ünal, Bünyamin</creator><general>Türk Gastroenteroloji Vakfı</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>GIY</scope><scope>GIZ</scope><scope>GJA</scope><scope>GJB</scope></search><sort><creationdate>20101201</creationdate><title>Effects of hypertension and ovariectomy on rat hepatocytes. Are amlodipine and lacidipine protective? (A stereological and histological study)</title><author>Dursun, Hakan ; Albayrak, Fatih ; Uyanik, Abdullah ; Keleş, Nuri Osman ; Beyzagül, Polat ; Bayram, Ednan ; Halici, Zekai ; Altunkaynak, Zuhal Berrin ; Süleyman, Halis ; Okçu, Nihat ; Ünal, Bünyamin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c314t-79fe1bc5095f04c3b0a01674f6710e9f1d122268ad1c17f610aef436c0d8e7013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Amlodipine - pharmacology</topic><topic>Animals</topic><topic>Calcium Channel Blockers - pharmacology</topic><topic>Cytoprotection - drug effects</topic><topic>Dihydropyridines - pharmacology</topic><topic>Female</topic><topic>Hepatocytes - drug effects</topic><topic>Hepatocytes - pathology</topic><topic>Hypertension - drug therapy</topic><topic>Liver - drug effects</topic><topic>Liver - pathology</topic><topic>Ovariectomy</topic><topic>Postmenopause</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dursun, Hakan</creatorcontrib><creatorcontrib>Albayrak, Fatih</creatorcontrib><creatorcontrib>Uyanik, Abdullah</creatorcontrib><creatorcontrib>Keleş, Nuri Osman</creatorcontrib><creatorcontrib>Beyzagül, Polat</creatorcontrib><creatorcontrib>Bayram, Ednan</creatorcontrib><creatorcontrib>Halici, Zekai</creatorcontrib><creatorcontrib>Altunkaynak, Zuhal Berrin</creatorcontrib><creatorcontrib>Süleyman, Halis</creatorcontrib><creatorcontrib>Okçu, Nihat</creatorcontrib><creatorcontrib>Ünal, Bünyamin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>ULAKBIM - Mühendislik ve Temel Bilimler Veri Tabani</collection><collection>ULAKBIM - Yaşam Bilimleri Veri Tabani</collection><collection>ULAKBIM - Turk Sosyal Bilimler Veri Tabani</collection><collection>ULAKBIM - Türk Tıp Veri Tabani</collection><jtitle>The Turkish journal of gastroenterology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dursun, Hakan</au><au>Albayrak, Fatih</au><au>Uyanik, Abdullah</au><au>Keleş, Nuri Osman</au><au>Beyzagül, Polat</au><au>Bayram, Ednan</au><au>Halici, Zekai</au><au>Altunkaynak, Zuhal Berrin</au><au>Süleyman, Halis</au><au>Okçu, Nihat</au><au>Ünal, Bünyamin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of hypertension and ovariectomy on rat hepatocytes. Are amlodipine and lacidipine protective? (A stereological and histological study)</atitle><jtitle>The Turkish journal of gastroenterology</jtitle><addtitle>Turk J Gastroenterol</addtitle><date>2010-12-01</date><risdate>2010</risdate><volume>21</volume><issue>4</issue><spage>387</spage><epage>395</epage><pages>387-395</pages><issn>1300-4948</issn><eissn>2148-5607</eissn><abstract>Calcium channel blockers are increasingly used for the treatment of hypertension. Menopause and hypertension are both important risk factors for liver damage and several other circulatory abnormalities. The aim of this study was to determine the effects of amlodipine and lacidipine in an ovariectomy-induced postmenopausal period model and a deoxycorticosterone acetate-salt-induced hypertensive model in rats.
In this study, animals were divided into six groups as follows: control (Group 1), hypertension (Group 2), ovariectomy (Group 3), ovariectomy and hypertension (Group 4), ovariectomy, hypertension and amlodipine-treated (Group 5), and ovariectomy, hypertension and lacidipine-treated (Group 6). At the end of the experiment, the livers were removed and tissue samples were histologically and stereologically examined.
The numerical densities of the hepatocytes according to group were 0.000422, 0.00329, 0.000272, 0.00259, 0.00374 and 0.000346 μm3, respectively. Significant differences were found between values of all groups (p<0.01, Mann-Whitney U test). According to histopathological investigation, Group 3 and particularly Group 4 showed some microscopic abnormalities such as dilatation in sinusoids central veins and branches of portal vein, irregularities of the hepatocyte columns, significant mononuclear cell infiltrations, and unstained vacuoles in the cytoplasm of the hepatocytes. Histological structure was protected from the destructive effects of ovariectomy and hypertension in Groups 5 and 6.
Our experimental results show that both hypertension and the postmenopausal period have negative effects on the number of hepatocytes and histological structure of the liver. Both amlodipine and lacidipine appear to ameliorate the hypertension and/or postmenopausal period-related decrease in hepatocyte number. We thus suggest that lacidipine and particularly amlodipine have important protective and recovering effects on the liver.</abstract><cop>Turkey</cop><pub>Türk Gastroenteroloji Vakfı</pub><pmid>21331992</pmid><doi>10.4318/tjg.2010.0125</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amlodipine - pharmacology Animals Calcium Channel Blockers - pharmacology Cytoprotection - drug effects Dihydropyridines - pharmacology Female Hepatocytes - drug effects Hepatocytes - pathology Hypertension - drug therapy Liver - drug effects Liver - pathology Ovariectomy Postmenopause Rats Rats, Inbred Strains |
| title | Effects of hypertension and ovariectomy on rat hepatocytes. Are amlodipine and lacidipine protective? (A stereological and histological study) |
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