Molecular characterization of Ornithobacterium rhinotracheale isolated from broiler chicken flocks in Iran
The present study was designed to isolate Ornithobacterium rhinotracheale in broiler flocks in Iran and characterize it using biochemical, polymerase chain reaction assays, and phylogenetic analyses. A total of 150 tracheal swabs were collected from broiler chickens in slaughterhouses. Additionally,...
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| Veröffentlicht in: | Turkish journal of veterinary & animal sciences 2010-01, Vol.34 (4), p.373-378 |
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| container_title | Turkish journal of veterinary & animal sciences |
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| creator | HASSANZADEH, MOHAMMAD KARRIMI, VAHID FALLAH, NEDA ASHRAFI, IRADJ |
| description | The present study was designed to isolate Ornithobacterium rhinotracheale in broiler flocks in Iran and
characterize it using biochemical, polymerase chain reaction assays, and phylogenetic analyses. A total of 150 tracheal
swabs were collected from broiler chickens in slaughterhouses. Additionally, 150 lung and 150 tracheal tissue samples were
taken from dead birds of broiler flocks that were developing symptoms of respiratory diseases. For PCR assay DNA was
extracted from ORT isolates and also from tissue samples. ORT was isolated from 1 out of 150 swabs samples and 3 out
of 300 total tissue samples. Four ORT isolates together with 7 DNA extractions from tissue samples generated
amplification products of 784 bp after the PCR with specific primers. Finally, it was confirmed that the sequence analysis
of 16S rRNA and ORT isolates is close to the isolates from GenBank with identity ranging from 98% to 100%. |
| doi_str_mv | 10.3906/vet-0810-19 |
| format | Article |
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characterize it using biochemical, polymerase chain reaction assays, and phylogenetic analyses. A total of 150 tracheal
swabs were collected from broiler chickens in slaughterhouses. Additionally, 150 lung and 150 tracheal tissue samples were
taken from dead birds of broiler flocks that were developing symptoms of respiratory diseases. For PCR assay DNA was
extracted from ORT isolates and also from tissue samples. ORT was isolated from 1 out of 150 swabs samples and 3 out
of 300 total tissue samples. Four ORT isolates together with 7 DNA extractions from tissue samples generated
amplification products of 784 bp after the PCR with specific primers. Finally, it was confirmed that the sequence analysis
of 16S rRNA and ORT isolates is close to the isolates from GenBank with identity ranging from 98% to 100%.</description><identifier>ISSN: 1303-6181</identifier><identifier>ISSN: 1300-0128</identifier><identifier>EISSN: 1303-6181</identifier><identifier>DOI: 10.3906/vet-0810-19</identifier><language>eng</language><publisher>TÜBİTAK</publisher><subject>bacterial diseases ; bakteriyel hastalıklar ; broilers ; DNA ; Et Veren Hayvanlar ; etlik piliç ; filogenetik ; filogeni ; Genetics and Molecular Biology of Microorganisms ; Hayvanların Prion, Viral, Bakteriyel ve Fungal Patojenleri ; İran ; kümes hayvanları ; Meat Producing Animals ; Mikroorganizmaların Genetiği ve Moleküler Biyolojisi ; molecular epidemiology ; moleküler epidemiyoloji ; nucleotide sequences ; nükleotit dizileri ; Ornithobacterium rhinotracheale ; phylogenetics ; phylogeny ; polimeraz zincir reaksiyonu ; polymerase chain reaction ; poultry ; Prion, Viral, Bacterial and Fungal Pathogens of Animals ; ribosomal RNA ; ribozomal RNA ; Taksonomi ve Evrim ; Taxonomy and Evolution</subject><ispartof>Turkish journal of veterinary & animal sciences, 2010-01, Vol.34 (4), p.373-378</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c292t-7b6f43cca7891d400f7f576486461a448a218712cf8596963339d4842ed03c9f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids></links><search><creatorcontrib>HASSANZADEH, MOHAMMAD</creatorcontrib><creatorcontrib>KARRIMI, VAHID</creatorcontrib><creatorcontrib>FALLAH, NEDA</creatorcontrib><creatorcontrib>ASHRAFI, IRADJ</creatorcontrib><title>Molecular characterization of Ornithobacterium rhinotracheale isolated from broiler chicken flocks in Iran</title><title>Turkish journal of veterinary & animal sciences</title><description>The present study was designed to isolate Ornithobacterium rhinotracheale in broiler flocks in Iran and
characterize it using biochemical, polymerase chain reaction assays, and phylogenetic analyses. A total of 150 tracheal
swabs were collected from broiler chickens in slaughterhouses. Additionally, 150 lung and 150 tracheal tissue samples were
taken from dead birds of broiler flocks that were developing symptoms of respiratory diseases. For PCR assay DNA was
extracted from ORT isolates and also from tissue samples. ORT was isolated from 1 out of 150 swabs samples and 3 out
of 300 total tissue samples. Four ORT isolates together with 7 DNA extractions from tissue samples generated
amplification products of 784 bp after the PCR with specific primers. Finally, it was confirmed that the sequence analysis
of 16S rRNA and ORT isolates is close to the isolates from GenBank with identity ranging from 98% to 100%.</description><subject>bacterial diseases</subject><subject>bakteriyel hastalıklar</subject><subject>broilers</subject><subject>DNA</subject><subject>Et Veren Hayvanlar</subject><subject>etlik piliç</subject><subject>filogenetik</subject><subject>filogeni</subject><subject>Genetics and Molecular Biology of Microorganisms</subject><subject>Hayvanların Prion, Viral, Bakteriyel ve Fungal Patojenleri</subject><subject>İran</subject><subject>kümes hayvanları</subject><subject>Meat Producing Animals</subject><subject>Mikroorganizmaların Genetiği ve Moleküler Biyolojisi</subject><subject>molecular epidemiology</subject><subject>moleküler epidemiyoloji</subject><subject>nucleotide sequences</subject><subject>nükleotit dizileri</subject><subject>Ornithobacterium rhinotracheale</subject><subject>phylogenetics</subject><subject>phylogeny</subject><subject>polimeraz zincir reaksiyonu</subject><subject>polymerase chain reaction</subject><subject>poultry</subject><subject>Prion, Viral, Bacterial and Fungal Pathogens of Animals</subject><subject>ribosomal RNA</subject><subject>ribozomal RNA</subject><subject>Taksonomi ve Evrim</subject><subject>Taxonomy and Evolution</subject><issn>1303-6181</issn><issn>1300-0128</issn><issn>1303-6181</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNpNkM1LxDAUxIMouKx78izkLtW85qPJURY_Flb2oueSpgnNbttIkhX0r7dLBT3NY2YYeD-EroHcUUXE_afNBZFAClBnaAGU0EKAhPN_9yVapbQnhIBSnHO1QPvX0Ftz7HXEptNRm2yj_9bZhxEHh3dx9LkLzewfBxw7P4Y89Tqre4t9Cr3OtsUuhgE3Mfjenpa8OdgRuz6YQ8J-xJuoxyt04XSf7OpXl-j96fFt_VJsd8-b9cO2MKUqc1E1wjFqjK6kgpYR4irHK8GkYAI0Y1KXICsojZNcCSUopaplkpW2JdQoR5foZt6dvjo0fqg_oh90_KoBOJF8ym_n3MSQUrTur0DqE8l6IlmfSNag6A-8lGdJ</recordid><startdate>20100101</startdate><enddate>20100101</enddate><creator>HASSANZADEH, MOHAMMAD</creator><creator>KARRIMI, VAHID</creator><creator>FALLAH, NEDA</creator><creator>ASHRAFI, IRADJ</creator><general>TÜBİTAK</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20100101</creationdate><title>Molecular characterization of Ornithobacterium rhinotracheale isolated from broiler chicken flocks in Iran</title><author>HASSANZADEH, MOHAMMAD ; KARRIMI, VAHID ; FALLAH, NEDA ; ASHRAFI, IRADJ</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c292t-7b6f43cca7891d400f7f576486461a448a218712cf8596963339d4842ed03c9f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>bacterial diseases</topic><topic>bakteriyel hastalıklar</topic><topic>broilers</topic><topic>DNA</topic><topic>Et Veren Hayvanlar</topic><topic>etlik piliç</topic><topic>filogenetik</topic><topic>filogeni</topic><topic>Genetics and Molecular Biology of Microorganisms</topic><topic>Hayvanların Prion, Viral, Bakteriyel ve Fungal Patojenleri</topic><topic>İran</topic><topic>kümes hayvanları</topic><topic>Meat Producing Animals</topic><topic>Mikroorganizmaların Genetiği ve Moleküler Biyolojisi</topic><topic>molecular epidemiology</topic><topic>moleküler epidemiyoloji</topic><topic>nucleotide sequences</topic><topic>nükleotit dizileri</topic><topic>Ornithobacterium rhinotracheale</topic><topic>phylogenetics</topic><topic>phylogeny</topic><topic>polimeraz zincir reaksiyonu</topic><topic>polymerase chain reaction</topic><topic>poultry</topic><topic>Prion, Viral, Bacterial and Fungal Pathogens of Animals</topic><topic>ribosomal RNA</topic><topic>ribozomal RNA</topic><topic>Taksonomi ve Evrim</topic><topic>Taxonomy and Evolution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HASSANZADEH, MOHAMMAD</creatorcontrib><creatorcontrib>KARRIMI, VAHID</creatorcontrib><creatorcontrib>FALLAH, NEDA</creatorcontrib><creatorcontrib>ASHRAFI, IRADJ</creatorcontrib><collection>CrossRef</collection><jtitle>Turkish journal of veterinary & animal sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HASSANZADEH, MOHAMMAD</au><au>KARRIMI, VAHID</au><au>FALLAH, NEDA</au><au>ASHRAFI, IRADJ</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterization of Ornithobacterium rhinotracheale isolated from broiler chicken flocks in Iran</atitle><jtitle>Turkish journal of veterinary & animal sciences</jtitle><date>2010-01-01</date><risdate>2010</risdate><volume>34</volume><issue>4</issue><spage>373</spage><epage>378</epage><pages>373-378</pages><issn>1303-6181</issn><issn>1300-0128</issn><eissn>1303-6181</eissn><abstract>The present study was designed to isolate Ornithobacterium rhinotracheale in broiler flocks in Iran and
characterize it using biochemical, polymerase chain reaction assays, and phylogenetic analyses. A total of 150 tracheal
swabs were collected from broiler chickens in slaughterhouses. Additionally, 150 lung and 150 tracheal tissue samples were
taken from dead birds of broiler flocks that were developing symptoms of respiratory diseases. For PCR assay DNA was
extracted from ORT isolates and also from tissue samples. ORT was isolated from 1 out of 150 swabs samples and 3 out
of 300 total tissue samples. Four ORT isolates together with 7 DNA extractions from tissue samples generated
amplification products of 784 bp after the PCR with specific primers. Finally, it was confirmed that the sequence analysis
of 16S rRNA and ORT isolates is close to the isolates from GenBank with identity ranging from 98% to 100%.</abstract><pub>TÜBİTAK</pub><doi>10.3906/vet-0810-19</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Turkish journal of veterinary & animal sciences, 2010-01, Vol.34 (4), p.373-378 |
| issn | 1303-6181 1300-0128 1303-6181 |
| language | eng |
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| source | EZB-FREE-00999 freely available EZB journals; TÜBİTAK Scientific Journals |
| subjects | bacterial diseases bakteriyel hastalıklar broilers DNA Et Veren Hayvanlar etlik piliç filogenetik filogeni Genetics and Molecular Biology of Microorganisms Hayvanların Prion, Viral, Bakteriyel ve Fungal Patojenleri İran kümes hayvanları Meat Producing Animals Mikroorganizmaların Genetiği ve Moleküler Biyolojisi molecular epidemiology moleküler epidemiyoloji nucleotide sequences nükleotit dizileri Ornithobacterium rhinotracheale phylogenetics phylogeny polimeraz zincir reaksiyonu polymerase chain reaction poultry Prion, Viral, Bacterial and Fungal Pathogens of Animals ribosomal RNA ribozomal RNA Taksonomi ve Evrim Taxonomy and Evolution |
| title | Molecular characterization of Ornithobacterium rhinotracheale isolated from broiler chicken flocks in Iran |
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