The effects of different anaesthetic agents on electrodiagnostic parameters in rats

The purpose of this study was to determine the possible effects of different anaesthetic agents on electrodiagnostic parameters in rectus abdominis, diaphragm and intercostal muscles which represent the muscles of trunk and respiratory, respectively in rat. For this purpose, ten adult, male, Sprague-Dawley rats were anaesthetized with ether, and rectus abdominis, intercostal and diaphragm muscle electromyographic (EMG) activities also with latency of n. phrenicus and n. ischiadicus were recorded in sequence by using concentric needle electrodes and surface stimulator. The same procedure was applied with xylazine/ketamine and fentanyl citrate/ketamine combinations at one week interval. Results of electrodiagnostic findings related to different anaesthetics in the same animal were compared and it was seen that parameters were different among the groups. Nerve conduction studies showed that ether and xylazine/ketamine caused significantly (P< 0.01) prolongation of motor nerve action potential latency (MNAPL) of nervus ischiadicus. Although the prolongation of MNAPL of n. phrenicus was observed in xylazine/ketamine group, it was not significantly different between groups. There was no significant difference on the EMG activity of rectus abdominis, intercostal and diaphragm muscles following the administration of ether, xylazine/ketamine and fentanyl/ketamine. This study showed some evidence about the effects of different anaesthetics on some electrodiagnostic parameters in rats. In electrophysiological studies using anaesthetics to keep the animals immobile, it will be kept in mind that the different anaesthetics may have influence on the nerve conduction latency. Bu çalışmanın amacı, ratlarda göğüs ve solunum kasları olan rectus abdominis, diyafram ve interkostal kasların elektrodiyagnostik parametreleri üzerine farklı anestezik maddelerin olası etkilerini belirlemektir. Bu amaçla 10 adet yetişkin, erkek Sprague-Dawley rat, eter ile anesteziye alındı, konsantrik iğne elektrodlar ve yüzeysel uyarıcılar kullanılarak, rectus abdominis, interkostal ve diyafram kaslarının elektromyografik (EMG) aktiviteleri ile birlikte n. phrenicus ve n. ischiadicus'un latansları kaydedildi. Aynı uygulama, birer hafta arayla ksilazin/ketamin ve fentanil/ketamin kombinasyonları ile tekrarlandı. Aynı hayvanlar üzerinde farklı anesteziklerle ilgili elektrodiyagnostik bulgular karşılaştırıldı ve gruplar arasında farklılıkların olduğu görüldü. Sinir iletim çalışmaları, eter ve ksilazi